Production of putrescine-capped stable silver nanoparticle: its characterization and antibacterial activity against multidrug-resistant bacterial strains

Abstract

Integration of biology with nanotechnology is now becoming attention-grabbing area of research. The antimicrobial potency of silver has been eminent from antiquity. Due to the recent desire for the enhancement of antibacterial efficacy of silver, various synthesis methods of silver in their nano dimensions are being practiced using a range of capping material. The present work highlights a facile biomimetic approach for production of silver nanoparticle being capped and stabilized by putrescine, possessing a diameter of 10-25 ± 1.5 nm. The synthesized nanoparticles have been analyzed spectrally and analytically. Morphological studies are carried out by high-resolution transmission electron microscopy and crystallinity by selected area electron diffraction patterns. Moreover, the elemental composition of the capped nanoparticles was confirmed by energy-dispersive X-ray spectroscopy analysis. A comparative study (zone of inhibition and minimum inhibitory concentration) regarding the interactions and antibacterial potentiality of the capped silver nanoparticles with respect to the bare ones reveal the efficiency of the capped one over the bare one. The bacterial kinetic study was executed to monitor the interference of nanoparticles with bacterial growth rate. The results also highlight the efficacy of putrescine-capped silver nanoparticles as effective growth inhibitors against multi-drug resistant human pathogenic bacterial strains, which may, thus, potentially be applicable as an effective antibacterial control system to fight diseases.

Augmented antifungal potential of benzothiazol-2-ylcarbamodithioates as hybrid-silver aqua nanoformulations

“…The AgNps also affect the bioefficacy by acting as carrier, taking the active molecules into the interior of the diseased tissues with favourable effects (Sevilla et al 2009). Our results were on the same line as the previous reported literature which indicated the effect of nanoconjugation on the bioefficacy non nanoformulations of bioactive compounds (Saha et al 2016;Vyas et al 2015;Kumar and Poornachandra 2015).…”

“…There was multifold enhancement in the fungitoxicity potential of AgNps when capped with BCDTC revealing favourable effect of conjugation. The results are in consonance with the earlier reports where the AgNP conjugates with most active compounds gave augmented biopotential than the bare ones (Saha et al 2016;Vyas et al 2015;Kumar and Poornachandra 2015), even against resistant strains of micro-organisms (Gajbhiye et al 2009;Tiwari et al 2014).…”

“…The silver nanoparticles were synthesized by chemical reduction method in all the samples. During addition of capping agent they were sonicated resulting in their further dispersal to some extent and capping molecules do not allow their aggregation leading to their reduced size (Saha et al 2016).…”

“…Nanotechnical methodologies of application of bioactive materials as their conjugates with metal nanocarriers (Kora and Rastogi 2013) or their loading on metal nanoparticles is a matter of intense interest (Duncan 2003;Ferrari 2005) and most pertinently emerging field. Nanoconjugates showed significant enhancement in biological activity in comparison to free drug molecules because of dispersion of organic molecules on the metal nanoparticle surface (Bordes et al 2009;Gajbhiye et al 2009;Saha et al 2016). This is also attributable to advanced morpho-topological properties as well as inherent biological properties of metal nanoparticles (Kanhed et al 2014;Liversidge et al 2003).…”

Inhibition of polyamine biosynthesis for toxicity control in Serratia marcescens strain WW4 by targeting ornithine decarboxylase: a structure-based virtual screening study

“…On the other hand being regulators for cell growth and death, it is likely that excess production of polyamines affects the severity and process of diseases. Putrescine, Spermidine and spermine are polyamines mainly found in microorganisms (Ahou et al, 2014;Miller-Fleming et al, 2015;Saha et al, 2016). Putrescine is deduced to Spermidine (Kraus et al, 2014) and finally down to spermine.…”