Plant-mediated RNAi of a gap gene-enhanced tobacco tolerance against the Myzus persicae

Mao, Jianjun; Zeng, Fanrong

doi:10.1007/s11248-013-9739-y

Cited by 96 publications

(77 citation statements)

References 43 publications

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“…Recently, Mao and Zhang [75] targeted the gap gene hunchback in M. persicae, which had previously been shown to be an effective target in A. pisum when fed in artificial diet. Feeding of transgenic tobacco expressing dsRNA targeting hunchback of M. persicae, however, elicited different results.…”

Section: Nilaparvata Lugensmentioning

confidence: 99%

RNA interference: Applications and advances in insect toxicology and insect pest management

Kim

Issa

Cooper

et al. 2015

Pesticide Biochemistry and Physiology

134

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Since its discovery, RNA interference (RNAi) has revolutionized functional genomic studies due to its sequence-specific nature of post-transcriptional gene silencing. In this paper, we provide a comprehensive review of the recent literature and summarize the current knowledge and advances in the applications of RNAi technologies in the field of insect toxicology and insect pest management. Many recent studies have focused on identification and validation of the genes encoding insecticide target proteins, such as acetylcholinesterases, ion channels, Bacillus thuringiensis receptors, and other receptors in the nervous system. RNAi technologies have also been widely applied to reveal the role of genes encoding cytochrome P450 monooxygenases, carboxylesterases, and glutathione S-transferases in insecticide detoxification and resistance. More recently, studies have focused on understanding the mechanism of insecticide-mediated up-regulation of detoxification genes in insects. As RNAi has already shown great potentials for insect pest management, many recent studies have also focused on host-induced gene silencing, in which several RNAi-based transgenic plants have been developed and tested as proof of concept for insect pest management. These studies indicate that RNAi is a valuable tool to address various fundamental questions in insect toxicology and may soon become an effective strategy for insect pest management.

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Section: Nilaparvata Lugensmentioning

confidence: 99%

RNA interference: Applications and advances in insect toxicology and insect pest management

Kim

Issa

Cooper

et al. 2015

Pesticide Biochemistry and Physiology

134

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“…The gene of different insects has been knock downed via orally fed dsRNA including insects from Hymenoptera (Lynch and Desplan 2006), Coleoptera (Tomoyasu et al 2008), Diptera (Dzitoyeva et al 2001), Lepidoptera (Terenius et al 2011). However, results from reports of Mao et al (2011), Zhu et al (2012 and Mao and Zeng (2014) are more encouraging who knock downed Fig. 2 The efficacy of insecticidal genes in transgenic cotton evaluated by leaf biotoxicity and artificial insect infestation of plants.…”

Section: Insect-resistant Cottonmentioning

confidence: 99%

An insight into cotton genetic engineering (Gossypium hirsutum L.): current endeavors and prospects

Bakhsh

Anayol²,

Özcan³

et al. 2015

Acta Physiol Plant

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Cotton (Gossypium hirsutum L.) is the most significant cash crop and backbone of global textile industry. The importance of cotton can hardly be over emphasized in the economy of cotton-growing countries as cotton and cotton products contribute significantly to the foreign exchange earnings. Cotton breeders have continuously sought to improve cotton's quality through conventional breeding in the past centuries; however, due to limited availability of germplasm with resistant to particular insects, pests and diseases, further advancements in cotton breeding have been challenging. The progress in transformation systems in cotton paved the way for the genetic improvement by enabling the researchers to transfer specific genes among the species and to incorporate them in cotton genome. With the development of first genetically engineered cotton plant in 1987, several characteristics such as biotic (insects, viruses, bacteria and fungi) resistance, abiotic (drought, chilling, heat, salt), herbicide tolerance, manipulation of oil and fiber traits have been reported to date. Genetic engineering has emerged as a necessary tool in cotton breeding programs, strengthening classical strategies to improve yield and yield contributing factors. The current review highlights the advances and endeavors in cotton genetic engineering achieved by researchers worldwide utilizing modern biotechnological approaches. Future prospects of the transgenic cotton are also discussed.

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“…dsRNA or mature siRNA can activate RNA degradation in insect cells, and different methods to deliver these molecules into whole aphids have been employed [12,18]. Aphids can be fed on dsRNA or siRNA sources, such as (1) in vitro-synthesized dsRNA [19,20,21,22,23,24,25,26]; (2) siRNA synthesized chemically or obtained after enzymatic cleavage of the dsRNA [27,28]; or (3) plants stably or transiently expressing dsRNA [29,30,31,32,33,34]. An alternative method to deliver dsRNA or siRNA into aphids is by microinjection, although this technique is not well adapted to a large scale analysis of genes [19,22,27,28,30,35,36,37].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of RNAi-Based Methods to Down-Regulate Expression of Two Genes Expressed at Different Levels in Myzus persicae

Mulot

Boissinot

Monsion

et al. 2016

Viruses

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With the increasing availability of aphid genomic data, it is necessary to develop robust functional validation methods to evaluate the role of specific aphid genes. This work represents the first study in which five different techniques, all based on RNA interference and on oral acquisition of double-stranded RNA (dsRNA), were developed to silence two genes, ALY and Eph, potentially involved in polerovirus transmission by aphids. Efficient silencing of only Eph transcripts, which are less abundant than those of ALY, could be achieved by feeding aphids on transgenic Arabidopsis thaliana expressing an RNA hairpin targeting Eph, on Nicotiana benthamiana infected with a Tobacco rattle virus (TRV)-Eph recombinant virus, or on in vitro-synthesized Eph-targeting dsRNA. These experiments showed that the silencing efficiency may differ greatly between genes and that aphid gut cells seem to be preferentially affected by the silencing mechanism after oral acquisition of dsRNA. In addition, the use of plants infected with recombinant TRV proved to be a promising technique to silence aphid genes as it does not require plant transformation. This work highlights the need to pursue development of innovative strategies to reproducibly achieve reduction of expression of aphid genes.

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Plant-mediated RNAi of a gap gene-enhanced tobacco tolerance against the Myzus persicae

Cited by 96 publications

References 43 publications

RNA interference: Applications and advances in insect toxicology and insect pest management

RNA interference: Applications and advances in insect toxicology and insect pest management

An insight into cotton genetic engineering (Gossypium hirsutum L.): current endeavors and prospects

Comparative Analysis of RNAi-Based Methods to Down-Regulate Expression of Two Genes Expressed at Different Levels in Myzus persicae

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