Plant Latex: A Rich Source of Haemostatic Proteases

Singh, Maheshwari Kumari; Sukumaran, Bindhu Omana

doi:10.1007/978-981-13-7248-3_10

Cited by 3 publications

(2 citation statements)

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“…Various enzymes have been widely investigated as antibiofilm agents, able to destroy the matrix and thereby increase the efficiency of antimicrobials against biofilm-embedded cells [33,[39][40][41][42][43]. Among proteases, plant latex proteases represent a promising class of prospective wound healing agents and enhancers of antibiofilm treatment [44,45]. Biofilm destruction properties in vitro have been reported for Ficin, a nonspecific sulfhydryl protease from Fig tree latex, Papain, a protease Papain from Papaya, and Bromelain from the pineapple against staphylococcal biofilms [26,28,31,46].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens

Baidamshina,

Trizna,

Goncharova

et al. 2023

IJMS

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In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19–32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by Streptococcus salivarius, Streptococcus gordonii, Streptococcus mutans, and Candida albicans, while no apparent effect on Staphylococcus aureus was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by Streptococcus sobrinus and S. gordonii. By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers S. gordonii and S. mutans. In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral Streptococci biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization.

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Section: Discussionmentioning

confidence: 99%

The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens

Baidamshina,

Trizna,

Goncharova

et al. 2023

IJMS

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“…It has been interpreted that the plant latex proteases are selectively interfering with blood coagulation factors and fibrinolytic cascade. These actions of plant proteases resulted in induction and dissolution of the final fibrin clot (Esimone et al, 2008;Dussourd and Eisner, 1987;Singh and Bindhu;Venkatesh et al, 2016). The complete mechanism of wound healing at its different stages by the plant latex proteases has been diagrammatically represented in the Figure 2 (Singh and Bindhu).…”

Section: Therapeutic Uses Of Plant Latexmentioning

confidence: 99%

Chemical bioactivity of plant latex as an herbal panacea

Das

Divya

Dubey

et al. 2022

ijhs

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Traditionally, latex containing plant existence have been viewed as the economical and health promotional entity from natural origin. Preliminary chemical investigations are based on protein or peptide content; along with many other bioactive smaller molecules targeting the health promotion by the management of enlisted disorders in this manuscript. It has also been observed different important metabolites such as, terpenoids, cardenolides, alkaloids having prominent antibacterial, antifungal, anthelmintic, insect repellent and cytotoxic activities. Latexes include distinct phytocomponents that are dissimilar to those involved in their bearing plants. Surveying the viability of plant latex from kind of bioactive chemical resource, this review article covers the chemical depiction of plant latexes for the health benefits of mankind. Subsequently, the recounted persona of various scientific research on latex from different plant origins encouraged the review to explore more about the plant latexes by highlighting their pharmacological benefits for health management.

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