Evidence of new calcium-dependent cysteine protease of Melia dubia latex with fibrinolytic potential

Background: Mimusops elengi, popularly known as Bakula in Ayurvedic Medicine, is a member of the Sapotaceae family. Concerning the traditional and Ayurvedic medicinal use of Mimusops elengi leaves, especially in wound healing and oral care, the plausible presence of proteolytic activity in an aqueous Mimusops elengi leaf extract was investigated in our study. Method: Mimusops elengi Gelatinolytic Protease was named after fractioning the extract. The Zymogram assay validated the gelatin specificity. The effect of MEGP on the wound healing process was investigated using a different assay. Result: The presence of protease with gelatinolytic & caseinolytic activity at 62.53±1.43 U/h and 15.31±0.64 U/h, respectively. The aqueous enzyme fraction was named Mimusops elengi Gelatinolytic Protease (MEGP). The specificity of gelatin was confirmed by zymogram. Gelatinolytic activity of MEGP was also higher than that of trypsin at 27.96±0.38 U/h. MEGP was 49.14±1.61 % inhibited by PMSF, indicating the predominant presence of serine proteases. The optimal pH and temperature for MEGP were found to be 8 and 37°C, implying that it is an alkaline protease. MEGP dissolved the blood clot at a rate of 8.41±1.04 U/h, which is higher than the trypsin clot-dissolving rate of 1.027±0.04 U/h. The plasma clot hydrolytic capacity is confirmed when MEGP hydrolyzes alpha-polymer subunits in a dose-dependent manner. Conclusion: This investigation shows that MEGP has a plasmin-like activity that possesses a significant role in clot disintegration and other wound ailments, warranting its use in traditional Indian medicine.

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Mimusops elengi (Bakula) Gelatinolytic Protease and its Plasmin-like Action on the Blood Clot

Achar

Nirmala

Rani

et al. 2022

CPPS

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Multifunctional and Smart Wound Dressings—A Review on Recent Research Advancements in Skin Regenerative Medicine

et al. 2022

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The healing of wounds is a dynamic function that necessitates coordination among multiple cell types and an optimal extracellular milieu. Much of the research focused on finding new techniques to improve and manage dermal injuries, chronic injuries, burn injuries, and sepsis, which are frequent medical concerns. A new research strategy involves developing multifunctional dressings to aid innate healing and combat numerous issues that trouble incompletely healed injuries, such as extreme inflammation, ischemic damage, scarring, and wound infection. Natural origin-based compounds offer distinct characteristics, such as excellent biocompatibility, cost-effectiveness, and low toxicity. Researchers have developed biopolymer-based wound dressings with drugs, biomacromolecules, and cells that are cytocompatible, hemostatic, initiate skin rejuvenation and rapid healing, and possess anti-inflammatory and antimicrobial activity. The main goal would be to mimic characteristics of fetal tissue regeneration in the adult healing phase, including complete hair and glandular restoration without delay or scarring. Emerging treatments based on biomaterials, nanoparticles, and biomimetic proteases have the keys to improving wound care and will be a vital addition to the therapeutic toolkit for slow-healing wounds. This study focuses on recent discoveries of several dressings that have undergone extensive pre-clinical development or are now undergoing fundamental research.

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Evidence of new calcium-dependent cysteine protease of Melia dubia latex with fibrinolytic potential

Cited by 2 publications

References 22 publications

Mimusops elengi (Bakula) Gelatinolytic Protease and its Plasmin-like Action on the Blood Clot

Mimusops elengi (Bakula) Gelatinolytic Protease and its Plasmin-like Action on the Blood Clot

Multifunctional and Smart Wound Dressings—A Review on Recent Research Advancements in Skin Regenerative Medicine

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