Plant GSTome: structure and functional role in xenome network and plant stress response

Labrou, Nikolaos E.; Papageorgiou, Anastassios C.; Pavli, Ourania I.; Flemetakis, Emmanouil

doi:10.1016/j.copbio.2014.12.024

Cited by 157 publications

(120 citation statements)

References 65 publications

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“…However, their abundance was not induced by heat stress in this study, as shown in previous heat stress studies (Shin et al, 2005; Lee et al, 2007; Wang et al, 2011, 2015). By contrast, GSTs and Trxs that were also involved in maintaining redox homeostasis in plants (Dixon et al, 2002; Park et al, 2009; Zhang et al, 2011; Zagorchev et al, 2013; Labrou et al, 2015), played a prominent role in redox regulation in our analysis. Accumulation of GST was thought to contribute to thermotolerance by reducing ROS production (Xu and Huang, 2008).…”

Section: Discussionmentioning

confidence: 62%

Comparative Proteomic Analysis of Flag Leaves Reveals New Insight into Wheat Heat Adaptation

Wang

et al. 2017

Front. Plant Sci.

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Hexaploid wheat (Triticum aestivum L.) is an important food crop but it is vulnerable to heat. The heat-responsive proteome of wheat remains to be fully elucidated because of previous technical and genomic limitations, and this has hindered our understanding of the mechanisms of wheat heat adaptation and advances in improving thermotolerance. Here, flag leaves of wheat during grain filling stage were subjected to high daytime temperature stress, and 258 heat-responsive proteins (HRPs) were identified with iTRAQ analysis. Enrichment analysis revealed that chlorophyll synthesis, carbon fixation, protein turnover, and redox regulation were the most remarkable heat-responsive processes. The HRPs involved in chlorophyll synthesis and carbon fixation were significantly decreased, together with severe membrane damage, demonstrating the specific effects of heat on photosynthesis of wheat leaves. In addition, the decrease in chlorophyll content may result from the decrease in HRPs involved in chlorophyll precursor synthesis. Further analysis showed that the accumulated effect of heat stress played a critical role in photosynthesis reduction, suggested that improvement in heat tolerance of photosynthesis, and extending heat tolerant period would be major research targets. The significantly accumulation of GSTs and Trxs in response to heat suggested their important roles in redox regulation, and they could be the promising candidates for improving wheat thermotolerance. In summary, our results provide new insight into wheat heat adaption and provide new perspectives on thermotolerance improvement.

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Section: Discussionmentioning

confidence: 62%

Comparative Proteomic Analysis of Flag Leaves Reveals New Insight into Wheat Heat Adaptation

Wang

et al. 2017

Front. Plant Sci.

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“…A lower K M value for GSH than the K M for CDNB was obtained by the kinetic analysis. The affinity for GSH and CDNB of other GSTs from different sources could vary even for different GST isoforms in the same species, because of gene duplication and divergent evolution [35, 41]. …”

Section: Resultsmentioning

confidence: 99%

Insights into ligand binding to a glutathione S-transferase from mango: Structure, thermodynamics and kinetics

et al. 2017

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We studied a mango glutathione S-transferase (GST) (Mangifera indica) bound to glutathione (GSH) and S-hexyl glutathione (GSX). This GST Tau class (MiGSTU) had a molecular mass of 25.5 kDa. MiGSTU Michaelis-Menten kinetic constants were determined for their substrates obtaining a Km, Vmax and kcat for CDNB of 0.792 mM, 80.58 mM·min−1 and 68.49 s−1 respectively and 0.693 mM, 105.32 mM·min−1 and 89.57 s−1, for reduced GSH respectively. MiGSTU had a micromolar affinity towards GSH (5.2 μM) or GSX (7.8 μM). The crystal structure of the MiGSTU in apo or bound to GSH or GSX generated a model that explains the thermodynamic signatures of binding and showed the importance of enthalpic-entropic compensation in ligand binding to Tau-class GST enzymes.

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“…A significant increase in GST activity and in the total antioxidant capacity (FRAP) in the blood plasma of goats (data not shown) was also observed when the animals consumed the SFO diet. GST is involved in the antioxidant defence system because it is responsible for the high‐capacity metabolic inactivation of electrophilic compounds including lipid hydroperoxides and toxic substrates (Board and Menon, ; Labrou et al., ).…”

Section: Resultsmentioning

confidence: 99%

Effect of soya bean and fish oil inclusion in diets on milk and plasma enzymes from sheep and goat related to oxidation

Tsiplakou

Chatzikonstantinou

Mitsiopoulou

et al. 2016

Animal Physiology Nutrition

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This study investigated the effects of dietary inclusion of soya bean oil combined with fish oil (SFO) on the activities of a) superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT) and glutathione transferase (GST) in blood plasma and b) SOD, GR, CAT and lactoperoxidase (LPO) in the milk of sheep and goats. Furthermore, the oxidative stress indicators for measuring total antioxidant activity and free radical scavenging activity [ferric reducing ability of plasma (FRAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays] and oxidative stress biomarkers [malondialdehyde (MDA) and protein carbonyl (PC)] were also determined in the blood plasma and milk of the animals. For this purpose, twelve dairy sheep and twelve dairy goats were assigned each to two homogenous subgroups. Treatments in both animal species involved a control diet without added oil and a diet supplemented with 5% soya bean oil and 1% fish oil. The results showed that the inclusion of SFO in the diets of sheep and goats increased significantly the activities of CAT and GR in their blood plasma. The same effect was observed for the activities of GST and FRAP in the blood plasma of goats. Moreover, the fact that the goats had significantly higher average daily PUFA intake (3.62 g/kg BW ) compared to sheep (2.51 g/kg BW ) resulted in an enhancement in the MDA content in their plasma. A significant increase in CAT activity in the milk in both animal species fed with SFO diets was also found. Finally, due to the higher apparent transfer rate of n-3 FA from the diet to the milk in sheep, the PC concentrations were found to be enhanced in their plasma and milk. In conclusion, the impact of dietary SFO supplementation on the oxidative status of body and/or on the milk of small ruminants depends not only on the daily PUFA intake, but also on the amount of n-3 FA that reach their milk.

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Plant GSTome: structure and functional role in xenome network and plant stress response

Cited by 157 publications

References 65 publications

Comparative Proteomic Analysis of Flag Leaves Reveals New Insight into Wheat Heat Adaptation

Comparative Proteomic Analysis of Flag Leaves Reveals New Insight into Wheat Heat Adaptation

Insights into ligand binding to a glutathione S-transferase from mango: Structure, thermodynamics and kinetics

Effect of soya bean and fish oil inclusion in diets on milk and plasma enzymes from sheep and goat related to oxidation

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