Plant growth promoting Pseudomonas aeruginosa from Valeriana wallichii displays antagonistic potential against three phytopathogenic fungi

Chandra, Harish; Kumari, Pragati; Bisht, Ravindra Singh; Prasad, Rajendra; Yadav, Saurabh

doi:10.1007/s11033-020-05676-0

Cited by 48 publications

(33 citation statements)

References 77 publications

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“…Several studies have demonstrated that P. aeruginosa causes rot and wilt (Clara 1930; Elrod and Braun 1942; Cother et al 1976; El-Said et al 1982; Bradbury 1986; Gupta et al 1986; Gao et al 2014; Tiwari and Singh 2017). On the other hand, plant-beneficial properties of agricultural P. aeruginosa, including a reduction in plant disease incidence have been reported in multiple investigations (Ali Siddiqui and Ehteshamul-Haque 2001; Adesemoye and Ugoji 2009; Yasmin et al 2014; Radhapriya et al 2015; Arif et al 2016; Durairaj et al 2017; Gupta and Buch 2019; Chandra et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The role of P. aeruginosa in the agricultural ecosystem is controversial. Several studies showed that P. aeruginosa supports plant growth and disease control through multiple mechanisms (Ali Siddiqui and Ehteshamul-Haque 2001; Adesemoye and Ugoji 2009; Yasmin et al 2014; Radhapriya et al 2015; Arif et al 2016; Durairaj et al 2017; Gupta and Buch 2019; Chandra et al 2020). P. aeruginosa helps in the solubilization of complex soil minerals (tri-calcium phosphate, potassium aluminum silicate, and zinc oxide), ammonification, and nitrification (Obaton et al 1968; Illmer and Schinner 1992; Fasim et al 2002; Jha et al 2009; Gupta and Buch 2019).…”

Section: Introductionmentioning

confidence: 99%

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Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Ambreetha

Marimuthu

Mathee

et al. 2021

Preprint

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Pseudomonas aeruginosa, a leading opportunistic pathogen causing hospital-acquired infections is predominantly present in agricultural settings. There are minimal attempts to examine the molecular and functional attributes shared by agricultural and clinical strains of P. aeruginosa. This study aims to investigate the presence of P. aeruginosa in edible vegetable plants (including salad vegetables) and analyze the evolutionary and metabolic relatedness of the agricultural and clinical strains. Eighteen rhizospheric and endophytic P. aeruginosa strains were isolated from cucumber, tomato, eggplant, and chili directly from the farms. The identity of these strains was confirmed using biochemical, and molecular markers and their genetic and metabolic traits were compared with clinical isolates. DNA fingerprinting analyses and 16S rDNA-based phylogenetic tree revealed that the plant- and human-associated strains are evolutionarily related. Both agricultural and clinical isolates possessed plant-beneficial properties, including mineral solubilization (phosphorous, potassium, and zinc), ammonification, and the ability to release extracellular siderophore and indole-3 acetic acid. These findings suggest that rhizospheric and endophytic P. aeruginosa strains are genetically and functionally analogous to the clinical isolates. This study highlights the edible plants as a potential source for human and animal transmission of P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Ambreetha

Marimuthu

Mathee

et al. 2021

Preprint

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“…Both P. aeruginosa and S. marcescens can be found in many natural environments, including the atmosphere, the soil, plants, water, and hospitals ( Besler and Little, 2017 ; Haddoudi et al, 2017 ; Chegini et al, 2020 ; Tan et al, 2020 ). Different strains of these species show diverse characteristics, with various strains having been proposed as pathogen/insect biocontrol agents, plant growth promotion bacteria (PGPBs) ( Singh and Jha, 2016 ; Haddoudi et al, 2017 ; Kumawat et al, 2019 ; Al-Ghafri et al, 2020 ; Chandra et al, 2020 ; Turhan et al, 2020 ), and opportunistic human pathogens ( Besler and Little, 2017 ; Tan et al, 2020 ). In particular, some P. aeruginosa strains show high intrinsic resistances to a range of antibiotics, resulting in significant morbidity and mortality rates ( Chegini et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Isolates of Pseudomonas aeruginosa and Serratia marcescens Associated With Post-harvest Fuzi (Aconitum carmichaelii) Rot and Their Novel Loop-Mediated Isothermal Amplification Detection Methods

et al. 2021

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Fuzi (the lateral root of Aconitum carmichaelii Debx.) is a traditional Chinese medicine that is cultivated in more than eight provinces in China. However, it can be easily devastated by post-harvest rot, causing huge losses. Therefore, it is extremely important that the primary causal pathogens of post-harvest Fuzi rot are identified and appropriate detection methods for them are developed to prevent and control losses. In this study, two bacterial strains (X1 and X2) were isolated from rotten post-harvest Fuzi. Based on their morphological, physiological, and biochemical characteristics, housekeeping gene homologies, and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS) results, these isolates were identified as Pseudomonas aeruginosa and Serratia marcescens. The pathogenicities of these isolates were confirmed by fulfilling Koch’s postulates demonstrating that they were post-harvest Fuzi rot pathogens. Two loop-mediated isothermal amplification (LAMP) methods targeting the gyrase B subunit (gyrB) gene of P. aeruginosa and the phosphatidylinositol glycan C (pigC) gene of S. marcescens were successfully developed, and it was found that the target genes were highly specific to the two pathogens. These LAMP methods were used to detect P. aeruginosa and S. marcescens in 46 naturally occurring Fuzi and their associated rhizosphere soil samples of unknown etiology. The two bacterial assays were positive in some healthy and rotten samples and could be accomplished within 1 h at 65°C without the need for complicated, expensive instruments. To our knowledge, this is the first report of P. aeruginosa and S. marcescens causing post-harvest Fuzi rot. The newly developed methods are expected to have applications in point-of-care testing for the two pathogens under different Fuzi planting procedures and will significantly contribute to the control and prevention of Fuzi rot.

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“…The use of microbial antagonists generally does not have a negative impact on the environment compared to synthetic fungicides ( Wartono et al., 2012 ). This microbe acting as a biocontrol is not only more economical than synthetic fungicides; it is also eco-friendly ( Chandra et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn

Mirsam

Kalqutny

Suriani

et al. 2021

Heliyon

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Indigenous fungi can suppress infection by pathogens and produce secondary metabolites that directly or indirectly affect plant growth. This study aimed to test indigenous fungi collected from corn plants as biological control agents and their effects on the viability and vigor of corn seeds. Purposive sampling method was used for sampling where soil samples taken from the rhizosphere zone, corn stem and leaf tissue from three locations namely Maros-South Sulawesi, Bone-South Sulawesi, Sigi-Central Sulawesi, Indonesia. Rhizospheric fungi were isolated from soil collected at the rhizosphere and rhizoplane using a serial dilution technique, while the endophytic fungi isolated from the leaves and stem tissues using surface sterilization method. The isolated fungi were cultured on a potato dextrose agar (PDA) medium. An antagonism test was performed using the dual culture method on PDA media with F. verticillioides as target pathogen. Pathogenicity test and the effect of fungi on corn seed germination was carried out using the blotter test method. Parameters observed were; necrotic symptoms on seedlings, growth potential, germination, growth rate, growth simultaneity, vigor index, germination rate, and time needed for 50% of the total germination. The effect of the isolated indigenous fungi on corn growth was carried out in-planta using seedling trays. The results of the blotter test and in-planta test were further confirmed by a physiological characteristic test. And assessing the fungi's ability to dissolve potassium, phosphate, and produce protease enzymes. A total of 89 fungal isolates were isolated and collected from various parts of the corn plant. Nineteen of the 89 fungal isolates showed inhibitory activity against F. verticillioides by ! 50% inhibition. The fungal isolates JRP 5 MRS, JRP 9 MRS, JRP 10 MRS, JRP 7 MRS, and JEDF 1B BN were selected based on the tests and showed a consistently positive effect on seed viability and vigor with a value of !90%. The isolates did not cause necrosis in corn, and had the ability to suppress the growth of pathogenic F. verticillioides by ! 50%.

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Plant growth promoting Pseudomonas aeruginosa from Valeriana wallichii displays antagonistic potential against three phytopathogenic fungi

Cited by 48 publications

References 77 publications

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Characteristics of Isolates of Pseudomonas aeruginosa and Serratia marcescens Associated With Post-harvest Fuzi (Aconitum carmichaelii) Rot and Their Novel Loop-Mediated Isothermal Amplification Detection Methods

Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn

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