Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Ambrosini, Adriana; Passaglia, Luciane Maria Pereira

doi:10.1002/pb.20054

Cited by 34 publications

(37 citation statements)

References 18 publications

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“…The PGP traits of K. variicola UC4115 and A. brasilense Sp7 were assessed following the methods described by Guerrieri et al (2020). Briefly, for qualitative estimation of tri-calcium phosphate solubilization, the strains were spot inoculated on GY/Tricalcium phosphate medium (Ambrosini and Passaglia, 2017), and incubated at 30 • C. After 7 days of incubation plates were observed for development of a clear halo zone around the colony, the halo's diameter was evaluated according to Ambrosini and Passaglia (2017). The phytohormone IAA production was estimated using the Salkowski reagent (12 g of FeCl 3 per L in 7.9 M H 2 SO 4 ) (Glickmann and Dessaux, 1995).…”

Section: In Vitro Assessment Of Pgp Traitsmentioning

confidence: 99%

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Guerrieri¹,

Fiorini²,

Fanfoni³

et al. 2021

Front. Plant Sci.

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Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination.

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Section: In Vitro Assessment Of Pgp Traitsmentioning

confidence: 99%

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Guerrieri¹,

Fiorini²,

Fanfoni³

et al. 2021

Front. Plant Sci.

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“…Considering the many activities of PGPR, it has been decided to start the screening analysis of bacteria that may fix atmospheric nitrogen using two different N-free semi-solid media, NFb (New Fábio Pedrosa) and LGI (Liquid Glucose Ivo) [41] and comparing the potential growth with the bacterial strain Azospirillum brasilense Sp7 (DSM 1690), used as positive control [42]. The isolation of the putative diazotrophic bacteria was carried out following the method proposed by Ambrosini and Passaglia [43]. After incubation, each suspension was diluted in a sterile NaCl 0.9% solution until reaching 10−3, in triplicates.…”

Section: Isolation Of Putative Diazotrophic Vacteriamentioning

confidence: 99%

Isolation and Screening of Extracellular PGPR from the Rhizosphere of Tomato Plants after Long-Term Reduced Tillage and Cover Crops

Guerrieri

Fanfoni

Fiorini

et al. 2020

Plants

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Plant growth promoting rhizobacteria provide an innovative solution to address challenges in sustainable agro-ecosystems, improving plant growth as well as acting as agents of biocontrol. In this study autochthonous bacteria were isolated from the rhizosphere of processing tomato plants (Solanum lycopersicum L.) cultivated with conservation agriculture practices (i.e., reduced tillage and cover crops), and evaluated for both growth-promoting activities (PGPAs), and antagonistic potential against the phytopathogenic pest Sclerotinia sclerotiorum. Considering the several activities of PGPR, we decided to structure the screening with a hierarchic approach, starting from testing the capability of fixing nitrogen. The obtained bacteria were processed through the molecular typing technique rep-PCR (Repetitive Extragenic Palindromic) in order to discriminate microbial strains with the same profiles, and identified via 16S rDNA sequencing. Thirty-eight selected isolates were screened in vitro for different activities related to plant nutrition and plant growth regulation as well as for antifungal traits. Isolated bacteria were found to exhibit different efficiencies in indoleacetic acid production and siderophore production, phosphate solubilization and biocontrol activity against the widespread soil-borne plant pathogen S. sclerotiorum. All the 38 bacterial isolates showed at least one property tested. With a view to detect the suitable candidates to be developed as biofertilizers, the selected isolates were ranked by their potential ability to function as PGPR. Thus, consortium of native PGPR bacteria inoculants may represent a suitable solution to address the challenges in sustainable agriculture, to ensure crop yield and quality, lowering the application of chemicals input.

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“…Several PGPB genera show association with different species of agricultural importance, such as Azospirillum, Bacillus and Pseudomonas (Zeffa et al, 2018). These bacteria can stimulate plant growth by a series of mechanisms, including but not restricted, the production of phytohormones, such as salicylic acid, gibberellins, cytokinins and indole-3-acetic acid (IAA) (Cassán and Diaz-Zorita, 2016;Fukami et al, 2017), phosphate solubilization (Ludueña et al, 2018;Qi et al, 2018), nutrient availability increase (Galindo et al, 2018b), production of indolic compounds and siderophores (Ambrosini and Passaglia, 2017), increase on 1−aminocyclopropane−1−carboxylate deaminase activity (Ambrosini and Passaglia, 2017), biological nitrogen fixation (BNF) (Pankievicz et al, 2015), biological control of plants, production of natural antibiotics and protective effect against secondary soil phytopathogens (Zhou et al, 2016;Mishra and Arora, 2018;Shameer and Prasad, 2018).…”

Section: Introductionmentioning

confidence: 99%

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

Pereira

Galindo

Gazola

et al. 2020

Front. Environ. Sci.

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The use of plant growth promoting bacteria (PGPB) that can solubilize phosphorus (P) has shown potential to improve nutrient availability in grass crops such as corn (Zea mays L.) This study was developed to investigate if inoculation with Azospirillum brasilense, Bacillus subtilis or Pseudomonas fluorescens associated with P 2 O 5 rates can improve phosphorus use efficiency (PUE) reflecting on greater corn development and yield. The field trial was set up in a Rhodic Hapludox under no-till system under Savannah conditions, in a completely randomized block design with four replicates. Treatments were tested in a full factorial design and included: (i) five P 2 O 5 rates (0 to 105 kg ha −1) and (ii) four PGPB seed inoculation (Control-without inoculation, A. brasilense, B. subtilis or P. fluorescens). Inoculation was found to increase grain yield by 39.5, 29.1, and 15.9% when B. subtilis was inoculated in the absence of P 2 O 5 rates and associated with 17.5 and 70 kg P 2 O 5 ha −1 and by 34.7% when A. brasilense was inoculated with application of 105 kg P 2 O 5 ha −1. In addition, inoculation with B. subtilis and A. brasilense were found to increase P uptake, benefiting productive components development, leading to an improved PUE, and greater corn grain yield. The results of this study showed positive improvements in P uptake as a result of B. subtilis and A. brasilense inoculation, with an increase of 100.5 and 54.6% on PUE, respectively; while the P. fluorescens inoculation were less evident. Further research should be conducted under biotic or/and abiotic conditions such as attack of pathogens and insects, drought, salinity, water flooding, low and high temperature to better understand the role of PGPB, inoculated alone or in combination as the co-inoculated method.

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Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Cited by 34 publications

References 18 publications

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant

Isolation and Screening of Extracellular PGPR from the Rhizosphere of Tomato Plants after Long-Term Reduced Tillage and Cover Crops

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

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