Plant genotype and growth regulators interaction affecting in vitro morphogenesis of blackberry and raspberry

Mezzetti, Bruno; Savini, G.; Carnevali, F.; Mott, D.

doi:10.1023/a:1000381612029

Cited by 24 publications

(8 citation statements)

References 25 publications

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“…ananassa that have been tested for in vitro regeneration and showing a different behaviour 19 . Several blackberry and raspberry lines have been tested for in vitro regeneration and showed different morphogenic responses to plant growth regulator combinations 20 . Our results showed that adventitious shoot regeneration of diploid strawberry F .…”

Section: Resultsmentioning

confidence: 99%

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Wang

Yang

et al. 2019

Sci Rep

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Diploid strawberry (Fragaria vesca ‘Baiguo’) is a model plant for studying functional genomics in Rosaceae. Adventitious shoot regeneration is essential for functional genomics by Agrobacterium tumefaciens-mediated transformation. An efficient shoot regeneration method using diploid strawberry leaf explants was conducted on 1/2MS + 1/2B5 medium that contained 2.0 mg L−1 TDZ over 14 days of dark culture; this induced the maximum percentage of shoot regeneration (96.44 ± 1.60%) and the highest number of shoots per explant (23.46 ± 2.14) after 11 weeks of culture. The explants considerably enlarged after 12 days; then, turned greenish brown after 30 days, yellowish brown after 36 days, and completely brown and necrotic after 48 days. Large numbers of adventitious shoots were produced from 48 to 66 days, and the shoots elongated from 66 to 78 days; this represents a critical period of reinvigoration, which included 30 days for leaf explant chlorosis, 36 days for adventitious shoot appearance, and 48 days for generation of numerous shoots. During the reinvigoration process, higher expressions of the hormone synthesis-related genes Ciszog1, CKX2, CKX3, CKX7, YUC2, YUC6, YUC10, YUC9, and GA2ox were detected from 30 to 48 days. Our results indicate that these genes may regulate reinvigoration of shoot regeneration.

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Section: Resultsmentioning

confidence: 99%

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Wang

Yang

et al. 2019

Sci Rep

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“…Protocols for in vitro plant regeneration from a number of Rubus genotypes, including blackberries, raspberries (R. idaeus L.), and their hybrids, are available (Cousineau and Donelly, 1991;Fiola and Swartz, 1986;Graham and McNicol, 1990;Mathews et al, 1995;Mezzetti et al, 1997;McNicol and Graham, 1990;Swartz et al, 1990;Tsao, 1999;Turk et HORTSCIENCE 39(2):316-320. 2004.…”

Section: Methodsmentioning

confidence: 99%

Improving In Vitro Plant Regeneration from Leaf and Petiole Explants of `Marion' Blackberry

Meng¹,

Chen²,

Finn³

et al. 2004

HortSci

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Experiments focusing on plant growth regulators' concentrations and combinations, mineral salt formulations, and TDZ pretreatment formations were conducted to optimize in vitro shoot regeneration from leaf and petiole explants of `Marion' blackberry. Optimum shoot formation was obtained when stock plants were incubated in TDZ pretreatment medium for 3 weeks before culturing leaf explants on regeneration medium (Woody Plant Medium with 5 μm BA and 0.5 μm IBA) in darkness for 1 week before transfer to light photoperiod (16-hour photoperiod at photosynthetic photon flux of ≈50 μmol·m-2·s-1) at 23 °C ± 2 °C for 4 weeks. Under these conditions, ≈70% of leaf explants formed ≈40 shoots per petri dish that could be harvested and rooted to form plantlets. Chemical names used: N6-benzyladenine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); gibberellic acid (GA3); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA); N-phenyl-N'-1,2,3-thidiazol-5-ylurea [thidiazuron (TDZ)].

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“…On the other hand, Cruz et al ( 2009 ) produced plant callus from leaves of Rubus adenotrichos Schltdl., using as growth promoters BA, IBA, NAA and kinetin in concentrations ranging between 0.5 and 2.0 mg/l, incubating the explants at 22 ± 2 °C with a 12 h light and 12 h dark photoperiod, where the best results were obtained using NAA and kinetin growth promoters. In the research performed by Mezzetti et al ( 1997 ), the effect of the growth regulators was evaluated in two species Rubus sp. From the regulators applied, the medium containing 5 µM of 2,4-D or 10 µM IBA, were the ones which, in all cases, promoted a higher callus production from leave explants.…”

Section: Discussionmentioning

confidence: 99%

Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis

et al. 2016

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Blackberries are fruits produced worldwide, with 25 % of their production centered in Mexico, Central and South America. Tropical highland blackberry is a fruit that can potentially enhance human health, due to their high content in phenolic compounds, which include anthocyanins, phenolic acids, tannins (gallotannins and elagitannins) and flavonoids. Therefore, the overall aim of this study is the development of a callus induction protocol, the establishment of blackberry cell suspensions (Rubus adenotrichos Schltdl.) and their cell analysis through optical microscopy and TEM, for the potential production of phenolic compounds. In order to produce callogenesis, segments of blackberry leaves were disinfected and placed in different concentrations of 2,4-D and the control media (0; 0.5; 1.0; 1.5; 2.0; 2.5 and 3.0 mg/l of 2,4-D); obtaining the higher size of calli in the medium with 1.5 mg/l of 2,4-D. After this determination, and for this specific treatment, a growth curve was performed through the use of fresh and dry weight parameters, in order to identify each of the growth stages. Furthermore, the calli obtained from the 1.5 mg/l of 2,4-D treatment were placed in two different culture media (MS and MS supplemented with 1.5 mg/l of 2,4-D) in order to establish the cell suspensions and the growth curve. To the best treatment, the total polyphenols were also quantified. It was determined that the MS medium is ideal for the growth and disintegration of the cell suspensions, obtaining 0.0256 mg of gallic acid/g of fresh sample. Finally, a cell callus and cell suspension analysis was performed through OM and TEM, evidencing a higher hystological differentiation in the calli, as well as the observation of antioxidant storage in the plastids.

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Plant genotype and growth regulators interaction affecting in vitro morphogenesis of blackberry and raspberry

Cited by 24 publications

References 25 publications

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Improving In Vitro Plant Regeneration from Leaf and Petiole Explants of `Marion' Blackberry

Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis

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