Improving In Vitro Plant Regeneration from Leaf and Petiole Explants of `Marion' Blackberry

Meng, Rengong; Chen, Chien-Jen; Finn, Chad E.; Li, Yonghai

doi:10.21273/hortsci.39.2.316

Cited by 33 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest regeneration rate was obtained on medium that, besides BAP, was supplemented with 0.1 mg l -1 IBA, which is in accordance with the results of the above authors. The increase in IBA concentration considerably reduces regeneration rate or, more importantly, inhibits it completely, which is in compliance with results obtained by Meng et al (2004). Adding 0.1 mg l -1 NAA, instead of IBA, into regeneration medium containing BAP considerably decreases regeneration rate.…”

Section: Discussionsupporting

confidence: 89%

“…BAP and TDZ are the most common cytokinins used for regeneration of different Rubus genotypes (Graham et al 1997;Mezzetti et al 1997;Meng et al 2004). The results of this study suggest that TDZ is more effective than BAP for inducing adventitious shoot regeneration from leaves of blackberry Č ačanska Bestrna, though a critical level of TDZ appears to be necessary for achieving high regeneration rate.…”

Section: Discussionmentioning

confidence: 62%

“…Adding 0.1 mg l -1 NAA, instead of IBA, into regeneration medium containing BAP considerably decreases regeneration rate. Also, Meng et al (2004) found that, when combined with 1.1 mg l -1 BAP, IBA at 0.1 mg l -1 was significantly better than similar concentration of IAA, NAA and 2,4-D for shoot regeneration of Marion blackberry.…”

Section: Discussionmentioning

confidence: 90%

“…Some studies on the influence of cytokinins on adventitious organogenesis suggested that 1.1-2.2 mg l -1 BAP alone or combined with 0.1 mg l -1 IBA is effective in improving regeneration from leaf explants in different blackberry cultivars (Mezzetti et al 1997;Graham et al 1997;Meng et al 2004). Nevertheless, our trial has shown that 2.0 mg l -1 BAP alone does not affect induction of adventitious organogenesis in blackberry Č ačanska Bestrna.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that regeneration of adventitious shoots in blackberry has been done from different types of explants, i.e. whole leaves Ambrožić Turk et al 1994;Gupta and Mahalaxmi 2009), leaf sections and petioles (Graham et al 1997;Mezzetti et al 1997;Meng et al 2004), excised cotyledons (Fiola et al 1990). Performed investigations have shown that shoot regeneration capacity varies widely among genotypes, which implies necessity to develop an optimised protocol for individual genotypes.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

et al. 2010

View full text Add to dashboard Cite

Adventitious shoot regeneration from leaves of blackberry cultivar Č ačanska Bestrna was examined using 30 different combinations of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS) medium containing either N 6 -benzylaminopurine (BAP) (2.0 mg l -1 ) or thidiazuron (TDZ) (1.0 and 2.0 mg l -1 ) alone, or either of them combined with indol-3-butyric acid (IBA), a-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l -1 ). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations of plant growth regulators. TDZ at 1.0 mg l -1 , applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in root tip meristems, and flow cytometry analysis indicated that adventitious shoots of Č ačanska Bestrna are tetraploid with 2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated plants). These differences were visible with 3,3 0 ,5,5 0 -tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

et al. 2010

View full text Add to dashboard Cite

show abstract

Blackberry Breeding and Genetics

Clark

Stafne

Hall

et al. 2007

Plant Breeding Reviews

Self Cite

View full text Add to dashboard Cite

Blackberry (Rubus L. subgenus Rubus Watson) improvement has made substantial progress with over 400 cultivars named originating from wild selections to many releases from breeding efforts. Public breeding has been ongoing for over 100 years. The result of these improvements is commercial production for processing and fresh markets in a number of countries. There has been excellent progress made in improving several very important traits. Fruit quality has been and continues to be a high priority in breeding, with selection for improved processing quality along with enhanced postharvest handling potential for fresh market expansion. Fruit size has been advanced and a range of berry sizes from small to very large exist among released cultivars. A number of plant characters have been addressed, with thornlessness becoming more common in recent introductions. Likewise, enhanced yield potential, improved disease resistance, and improved cane management characteristics have been achieved. More recently, primocane fruiting has been incorporated into blackberry, expanding production options. Breeding has been done using classical breeding methodology, crossing complementary parents and selecting improved progeny. Research using molecular methods has been limited in blackberry, and has not contributed substantially to cultivar improvement. Although breeding efforts in blackberry are more limited than the major berry crops, a continuous supply of new cultivars should result from ongoing programs. These, along with previous cultivar developments, will contribute to an ever-increasing number of cultivar options resulting in an increased of production of this increasingly popular berry crop.

show abstract

Berry Crops

Hancock

Song

Sink

2008

Compendium of Transgenic Crop Plants

View full text Add to dashboard Cite

Considerable genetic improvement has been achieved in the berry crops, mostly through conventional breeding. Strawberry breeders have focused on improving local adaptations, fruit quality, productivity, and disease resistance, and many are interested in developing day‐neutral cultivars. Transgenic strawberries have been produced with herbicide and pest resistances. A number of genes from strawberry have also been characterized that are associated with fruit ripening and maturation, and an effective marker‐free transformation process has been developed. The major objectives of blueberry breeders have centered on high plant vigor, improved disease resistance, flavor, longer fruit storage, and expanded harvest dates. Transgenic blueberries have been produced with herbicide resistance. Cranberry breeders have focused on early maturing fruit, uniform large size, intense color, keeping quality, high productivity, disease resistance, and plant vigor. The Bt gene ( Bacillus thuringiensis ) has been incorporated into cranberry through transformation. Raspberry breeders have been most interested in improving fruit quality and increasing the efficiency of fruit production. The development of primocane fruiting types has also been a high priority in many programs. Transformation was used to develop a red raspberry cultivar with resistance to Raspberry bushy dwarf virus. Blackberry breeders have been interested in increasing the climatic zone, improving harvest efficiency, reducing chilling requirements, improving fruit quality, and generating primocane fruiting types. Little transformation work has been done on blackberry.

show abstract

Improving In Vitro Plant Regeneration from Leaf and Petiole Explants of `Marion' Blackberry

Cited by 33 publications

References 22 publications

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

Blackberry Breeding and Genetics

Berry Crops

Contact Info

Product

Resources

About