Plant genetic transformation using carbon nanotubes for DNA delivery

Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

“…Recently several reports have demonstrated carbon nanoparticles as efficient delivery systems of plant biomolecules such as DNA, RNA, and protein and are capable of strong internalization in planta [49]. The carbon nanotubes enable plasmid delivery without transgene integration into crop species [58][59][60], which are expressed across different tissues including leaves, roots protoplast, and immature tissues [59]. Enhanced GFP expression in leaf protoplasts through carbon nanotube delivery has been reported in wheat (Triticum aestivum) and arugula (Eruca sativa) [50].…”

Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

“…Recently several reports have demonstrated carbon nanoparticles as efficient delivery systems of plant biomolecules such as DNA, RNA, and protein and are capable of strong internalization in planta [49]. The carbon nanotubes enable plasmid delivery without transgene integration into crop species [58][59][60], which are expressed across different tissues including leaves, roots protoplast, and immature tissues [59]. Enhanced GFP expression in leaf protoplasts through carbon nanotube delivery has been reported in wheat (Triticum aestivum) and arugula (Eruca sativa) [50].…”

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

“…Arginine‐functionalized SWCNTs have effectively transported GFP encoding pDNA into the tobacco intact root cells . In another effort to transport pDNA, Burlaka et al . have shown the internalization of pGreen 0029, a type of pDNA into the plant cell walls of N. tabacum using non‐covalent functionalized carbon nanotubes.…”

New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation

“…While N. tabacum callus and leaf explants were genetically transformed by the nptII gene contained in the pGreen 0029 construct, regenerated plants were obtained on a selective medium. The investigation of Burlaka et al (2015) demonstrated SWCNTs applicability for the transformation of protoplasts and walled plant cells. At the same time, MWCNTs demonstrated their applicability only for the transformation of protoplasts because of a limiting role of the cellulose wall against their penetration into the cells.…”

Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

“…Recently several reports have demonstrated carbon nanoparticles as efficient delivery systems of plant biomolecules such as DNA, RNA, and protein and are capable of strong internalization in planta [49]. The carbon nanotubes enable plasmid delivery without transgene integration into crop species [58][59][60], which are expressed across different tissues including leaves, roots protoplast, and immature tissues [59]. Enhanced GFP expression in leaf protoplasts through carbon nanotube delivery has been reported in wheat (Triticum aestivum) and arugula (Eruca sativa) [50].…”

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

“…Arginine‐functionalized SWCNTs have effectively transported GFP encoding pDNA into the tobacco intact root cells . In another effort to transport pDNA, Burlaka et al . have shown the internalization of pGreen 0029, a type of pDNA into the plant cell walls of N. tabacum using non‐covalent functionalized carbon nanotubes.…”

New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation

“…While N. tabacum callus and leaf explants were genetically transformed by the nptII gene contained in the pGreen 0029 construct, regenerated plants were obtained on a selective medium. The investigation of Burlaka et al (2015) demonstrated SWCNTs applicability for the transformation of protoplasts and walled plant cells. At the same time, MWCNTs demonstrated their applicability only for the transformation of protoplasts because of a limiting role of the cellulose wall against their penetration into the cells.…”

Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

“…Recently several reports have demonstrated carbon nanoparticles as efficient delivery systems of plant biomolecules such as DNA, RNA, and protein and are capable of strong internalization in planta [49]. The carbon nanotubes enable plasmid delivery without transgene integration into crop species [58][59][60], which are expressed across different tissues including leaves, roots protoplast, and immature tissues [59]. Enhanced GFP expression in leaf protoplasts through carbon nanotube delivery has been reported in wheat (Triticum aestivum) and arugula (Eruca sativa) [50].…”

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

“…Arginine‐functionalized SWCNTs have effectively transported GFP encoding pDNA into the tobacco intact root cells . In another effort to transport pDNA, Burlaka et al . have shown the internalization of pGreen 0029, a type of pDNA into the plant cell walls of N. tabacum using non‐covalent functionalized carbon nanotubes.…”

New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation

“…While N. tabacum callus and leaf explants were genetically transformed by the nptII gene contained in the pGreen 0029 construct, regenerated plants were obtained on a selective medium. The investigation of Burlaka et al (2015) demonstrated SWCNTs applicability for the transformation of protoplasts and walled plant cells. At the same time, MWCNTs demonstrated their applicability only for the transformation of protoplasts because of a limiting role of the cellulose wall against their penetration into the cells.…”