Plant genetic transformation using carbon nanotubes for DNA delivery

Burlaka, O. M.; Pirko, Ya. V.; Yemets, А. І.; Blume, Ya. B.

doi:10.3103/s009545271506002x

Cited by 68 publications

(31 citation statements)

References 35 publications

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“…Foreign particle uptake in plants can naturally occur either via endocytosis or by direct penetration. In plants, different engineered nanomaterials can be used for nanoparticle-mediated DNA transfer using gene-nanoparticle (NP) anchoring using zinc, calcium phosphate, silica, gold, magnetite, strontium phosphate, magnesium phosphate, and manganese phosphate (Sokolova and Epple, 2008;Mahendra et al, 2012) and carbon-based materials such as starch (Sun et al, 2009) fullerenes, single-walled carbon nanohorns (SWCNHs), singlewalled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs) (Burlaka et al, 2015) and dendrimers. However, it has been reported that nanoparticle uptake by plant cells undergoes faster when positively charged nanoparticles are used rather than negatively charged nanoparticles, perhaps due to the preference of the negatively charged cell wall for cations (Cunningham et al, 2018).…”

Section: Nano-carriers As a Non-viral Vector For Gene Delivery In Plamentioning

confidence: 99%

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Vega-Vásquez

Mosier

Irudayaraj

2020

Front. Bioeng. Biotechnol.

200

107

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The main challenges in drug delivery systems are to protect, transport and release biologically active compounds at the right time in a safe and reproducible manner, usually at a specific target site. In the past, drug nano-carriers have contributed to the development of precision medicine and to a lesser extent have focused on its inroads in agriculture. The concept of engineered nano-carriers may be a promising route to address confounding challenges in agriculture that could perhaps lead to an increase in crop production while reducing the environmental impact associated with crop protection and food production. The main objective of this review is to contrast the advantages and disadvantages of different types of nanoparticles and nano-carriers currently used in the biomedical field along with their fabrication methods to discuss the potential use of these technologies at a larger scale in agriculture. Here we explain what is the problem that nano-delivery systems intent to solve as a technological platform and describe the benefits this technology has brought to medicine. Also here we highlight the potential drawbacks that this technology may face during its translation to agricultural applications, based on the lessons learned so far from its use for biomedical purposes. We discuss not only the characteristics of an ideal nano-delivery system, but also the potential constraints regarding the fabrication including technical, environmental, and legal aspects. A key motivation is to evaluate the potential use of these systems in agriculture, especially in the area of plant breeding, growth promotion, disease control, and post-harvest quality control. Further, we highlight the importance of a rational design of nano-carriers and identify current research gaps to enable scale-up relevant to applications in the treatment of plant diseases, controlled release of fertilizers, and plant breeding.

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Section: Nano-carriers As a Non-viral Vector For Gene Delivery In Plamentioning

confidence: 99%

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Vega-Vásquez

Mosier

Irudayaraj

2020

Front. Bioeng. Biotechnol.

200

107

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“…The SWCNTs serve as gene-delivery vehicles that can penetrate the membranes of organelles and have many advantages in plant genetic engineering (Kwak et al, 2019), such as biocompatibility, high aspect ratio, high surface area to volume ratio and exceptional tensile strength (Hendler-Neumark and Bisker, 2019;Mohanta et al, 2019). As a result of their high surface area to volume ratio, SWCNTs can be loaded with large quantities of DNA for transfer into plant cells (Burlaka et al, 2015;Kwak et al, 2019;Demirer et al, 2019aDemirer et al, , 2019bDemirer et al, , 2019c 2019c). Finally, the cargo-nanoparticle complexes formed by SWCNTs and their loaded cargoes have strong intrinsic near-infrared (nIR) fluorescence, allowing them to be tracked in real time (Li et al, 2014;Hendler-Neumark and Bisker, 2019).…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Nanoparticle‐mediated gene transformation strategies for plant genetic engineering

Jiang

Chen

et al. 2020

The Plant Journal

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Plant genetic engineering, a recent technological advancement in the field of plant science, is an important tool used to improve crop quality and yield, to enhance secondary metabolite content in medicinal plants or to develop crops for sustainable agriculture. A new approach based on nanoparticle-mediated gene transformation can overcome the obstacle of the plant cell wall and accurately transfer DNA or RNA into plants to produce transient or stable transformation. In this review, several nanoparticle-based approaches are discussed, taking into account recent advances and challenges to hint at potential applications of these approaches in transgenic plant improvement programs. This review also highlights challenges in implementing the nanoparticle-based approaches used in plant genetic engineering. A new technology that improves gene transformation efficiency and overcomes difficulties in plant regeneration has been established and will be used for the de novo production of transgenic plants, and CRISPR/Cas9 genome editing has accelerated crop improvement. Therefore, we outline future perspectives based on combinations of genome editing, nanoparticle-mediated gene transformation and de novo regeneration technologies to accelerate crop improvement. The information provided here will assist an effective exploration of the technological advances in plant genetic engineering to support plant breeding and important crop improvement programs.

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“…Arginine-functionalized SWCNTs have effectively transported GFP encoding pDNA into the tobacco intact root cells. [83] In another effort to transport pDNA, Burlaka et al [12] have shown the internalization of pGreen 0029, a type of pDNA into the plant cell walls of N. tabacum using non-covalent functionalized carbon nanotubes. The engineered callus and leaf explants of N. tabacum achieved significant sprout regeneration with no toxicity at the indicated concentration.…”

Section: Plant Biotechnology and Agriculturementioning

confidence: 99%

“…Second, we will extend the point of discussion of this review towards the genetic engineering of plants using CNTs. [12] The structure of CNTs and their morphology is quite intriguing and worthy of discussion for agriculture and horticulture use. [13] These genetically modified crops barely require any chemicals for their optimum growth thus can easily achieve mass production.…”

Section: Introductionmentioning

confidence: 99%

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Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

et al. 2020

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The concept of gene vectors for therapeutic applications has been known for several years, but it is far from revealing its actual potential. With the advent of hollow cylindrical carbon nanomaterials such as carbon nanotubes (CNTs), researchers have invented several new tools to deliver genes at the required site of action in mammalian and plant cells. The ease of diversified functionalization has allowed CNTs to be by far the most adaptable non‐viral vector for gene therapy. This Minireview addresses the dexterity with which CNTs undergo surface modifications and their applications as a potent vector in gene therapy of humans and plants. Specifically, we will discuss the new tools that scientific communities have invented to achieve gene therapy using plasmid DNA, RNA silencing, suicide gene therapy, and plant genetic engineering. Additionally, we will shed some light on the mechanism of gene transportation using carbon nanotubes in cancer cells and plants.

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Plant genetic transformation using carbon nanotubes for DNA delivery

Cited by 68 publications

References 35 publications

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Nanoscale Drug Delivery Systems: From Medicine to Agriculture

Nanoparticle‐mediated gene transformation strategies for plant genetic engineering

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

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