Plant genetic diversity by DNA barcoding to investigate propolis origin

Sartori, Alan Giovanini de Oliveira; Cesar, Aline Silva Mello; Woitowicz, Franciélli Cristiane Gruchowski; Saliba, Ana Sofia Martelli Chaib; Ikegaki, Masaharu; Rosalen, Pedro Luiz; Alencar, Severino Matias de

doi:10.1016/j.phytochem.2022.113226

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Kopsia species typically contain potent and diversely bioactive indole alkaloid compounds. 3,11 The species Euchresta horsfieldii (Lesch.) Benn belongs to the genus Euchresta (family Leguminosae).…”

Section: Discussionmentioning

confidence: 99%

Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

Ariati¹,

Sasadara

Wirawan

et al. 2022

Bali Med J.

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Background: Purnajiwa is one of the medicinal plants in Bali. Species information regarding this plant is still unconfirmed. Currently, this plant is considered rare and endangered. Molecular identification supports authentication at the species level, which will also support conservation efforts. Method: Molecular identification using DNA Barcoding was carried out on purnajiwa from three sampling locations in Bali (Jimbaran, Mambal, and Bedugul) using rbcL primers (rbcLaF and rbcLaR). Data analysis was performed using BLAST with species on GenBank. Pairwise and similarity values were used to measure the three samples' proximity to the Gen Bank species. Result: The phylogenetic tree was constructed with the Maximum Likelihood and Tamura-3-parameter model. The analysis results show a close relationship between the purnajiwa from Jimbaran and Mambal with the species Kopsia arborea (KP095079). Meanwhile, the Purnajiwa from Bedugul showed a difference from the other two purnajiwa and was closely related to Euchresta horsfieldii (U74225). Conclusion: It can be concluded that the Purnajiwa from Jimbaran and Mambal is a species of Kopsia arborea while the Purnajiwa from Bedugul is a species of Euchresta horsfieldii.

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Section: Discussionmentioning

confidence: 99%

Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

Ariati¹,

Sasadara

Wirawan

et al. 2022

Bali Med J.

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“…There are several DNA barcode regions that can be used to authenticate medicinal plants. These include internal transcribed spacer (ITS), the intergenic spacer region (psbA-trnH), ribulose-1,5-bisphosphate carboxylase subunit (rbcL), and maturase K (matK) [11,12]. The chloroplast marker is considered as one of the core barcoding markers in plants [13].…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding application for potential medicinal plants on Ziziphus species from Aketajawe-Lolobata National Park, North Moluccas

Arifiani,

Sulistyaningsih,

Martiansyah

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Aketajawe-Lolobata National Park is a protected national park located in the province of North Moluccas, Indonesia. The national park has been known to be rich in biodiversity with the existence of many potential medicinal plants such as Ziziphus. DNA barcoding can be a useful tool for identifying and characterizing these plants for potential medical applications. Research and exploration have been conducted in Aketajawe-Lolobata National Park in November and December 2022. This study aimed to analyze how the DNA barcoding regions aid identification for potential medicinal plants. The genomic DNA were amplified by matK primers, and the sequences showed high similarity rate with their homologs, then the phylogenetic tree was constructed using the Neighbor-Joining method to assess their cross-species relationship. The phylogenetic study showed that the species that have been identified based on morphological characters, i.e., Ziziphus angustifolia and Z. mauritiana were clustered with Z. angustifolia and Z. mauritiana retrieved from GenBank. However, the unidentified species are closely related with Z. calophylla obtained from GenBank but with low bootstrap support. All the results indicate that DNA barcoding approach could be successfully used for reliable identification of medicinal plants and matK gene is a good candidate for this approach.

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Application of DNA barcoding to the entire traditional Chinese medicine industrial chain: A case study of Rhei Radix et Rhizoma

Xin

Lou

et al. 2022

Phytomedicine

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Plant genetic diversity by DNA barcoding to investigate propolis origin

Cited by 6 publications

References 41 publications

Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

Application of DNA Barcoding for authentication of Balinese traditional medicinal plant Purnajiwa (Kopsia arborea Blume. and Euchresta horsfieldii) (Lesch.) Benn

DNA barcoding application for potential medicinal plants on Ziziphus species from Aketajawe-Lolobata National Park, North Moluccas

Application of DNA barcoding to the entire traditional Chinese medicine industrial chain: A case study of Rhei Radix et Rhizoma

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