Plant-based porcine reproductive and respiratory syndrome virus VLPs induce an immune response in mice

Uribe-Campero, Laura; García, Alberto Monroy; Duran-Meza, Ana Luisa; Villagrana-Escareño, María V.; Ruíz-García, Jaime; Hernández, Jesús; Núñez-Palenius, Héctor G.; Gómez-Lim, Miguel Á.

doi:10.1016/j.rvsc.2015.07.012

Cited by 12 publications

(9 citation statements)

References 60 publications

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“…Moreover, VLPs are scalable under good manufacturing practice (GMP) conditions using varying strategies, including yeast, mammalian, and insect cell systems ( Table 1 ). Several studies have been conducted regarding the development of VLPs against different animal diseases with different outcomes, including PRRSV [ 53 , 54 ], swine influenza A virus [ 55 ], Newcastle virus [ 56 ], different fish viral diseases [ 57 ], and FMDV in cattle [ 58 ] and pigs [ 59 ].…”

Section: Nanovaccines: the New Era Of Vaccinationmentioning

confidence: 99%

Exosome-Based Vaccines: Pros and Cons in the World of Animal Health

Montaner-Tarbes

Fraile

Montoya

et al. 2021

Viruses

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Due to the emergence of antibiotic resistance and new and more complex diseases that affect livestock animal health and food security, the control of epidemics has become a top priority worldwide. Vaccination represents the most important and cost-effective measure to control infectious diseases in animal health, but it represents only 23% of the total global animal health market, highlighting the need to develop new vaccines. A recent strategy in animal health vaccination is the use of extracellular vesicles (EVs), lipid bilayer nanovesicles produced by almost all living cells, including both prokaryotes and eukaryotes. EVs have been evaluated as a prominent source of viral antigens to elicit specific immune responses and to develop new vaccination platforms as viruses and EVs share biogenesis pathways. Preliminary trials with lymphocytic choriomeningitis virus infection (LCMV), porcine reproductive and respiratory syndrome virus (PRRSV), and Marek’s disease virus (MDV) have demonstrated that EVs have a role in the activation of cellular and antibody immune responses. Moreover, in parasitic diseases such as Eimeria (chickens) and Plasmodium yoelii (mice) protection has been achieved. Research into EVs is therefore opening an opportunity for new strategies to overcome old problems affecting food security, animal health, and emerging diseases. Here, we review different conventional approaches for vaccine design and compare them with examples of EV-based vaccines that have already been tested in relation to animal health.

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Section: Nanovaccines: the New Era Of Vaccinationmentioning

confidence: 99%

Exosome-Based Vaccines: Pros and Cons in the World of Animal Health

Montaner-Tarbes

Fraile

Montoya

et al. 2021

Viruses

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“…According to the Centre for Food Security and Public Health of Iowa State University, only “BIOSUIS PRRS Inact EU+Am” is approved to be used in the US. However, new strategies are being evaluated to overcome these problems (115), including nanoparticle entrapped antigens (116–119), plant based approaches (120) or vectored vaccines (121).…”

Section: Immunology Of Prrsv and Mechanisms Involved In Immune Evasionmentioning

confidence: 99%

Key Gaps in the Knowledge of the Porcine Respiratory Reproductive Syndrome Virus (PRRSV)

et al. 2019

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The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important swine diseases in the world. It is causing an enormous economic burden due to reproductive failure in sows and a complex respiratory syndrome in pigs of all ages, with mortality varying from 2 to 100% in the most extreme cases of emergent highly pathogenic strains. PRRSV displays complex interactions with the immune system and a high mutation rate, making the development, and implementation of control strategies a major challenge. In this review, the biology of the virus will be addressed focusing on newly discovered functions of non-structural proteins and novel dissemination mechanisms. Secondly, the role of different cell types and viral proteins will be reviewed in natural and vaccine-induced immune response together with the role of different immune evasion mechanisms focusing on those gaps of knowledge that are critical to generate more efficacious vaccines. Finally, novel strategies for antigen discovery and vaccine development will be discussed, in particular the use of exosomes (extracellular vesicles of endocytic origin). As nanocarriers of lipids, proteins and nucleic acids, exosomes have potential effects on cell activation, modulation of immune responses and antigen presentation. Thus, representing a novel vaccination approach against this devastating disease.

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“…It clearly demonstrates that CSFV-MIP sensors bind CSFV with selectivity factors of 2 over PRRSV and 62 over PRV. Obviously, not only particle diameters determine selectivity: the average PRRSV particle diameter is d ¼ 70 nm (Uribe-Campero et al, 2015), which is somewhat larger than that of CSFV (d ¼ 50-60 nm) (Moennig, 2000). Given the size distribution, one can expect that some PRRSV virions fit into cavities on the CSFV-MIP, which leads to minor frequency responses to PRRSV.…”

Section: Selectivity and Reproducibilitymentioning

confidence: 99%

Sensing the classical swine fever virus with molecularly imprinted polymer on quartz crystal microbalance

Klangprapan

Choke-arpornchai

Lieberzeit

et al. 2020

Heliyon

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Classical swine fever (CSF) is a highly contagious and fatal viral disease in pigs caused by the virus of the same name (classical swine fever virus -CSFV). Economical reasons dictate the need for rapid early detection of this pathogen. Herein we report on a sensor for CSFV detection based on a quartz crystal microbalance (QCM) making use of molecularly imprinted polymer (MIP) as the receptor. It relies on a copolymer comprising acrylamide (AAM), methacrylic acid (MAA), methyl methacrylate (MMA), and n-vinylpyrrolidone (VP). SEM images of CSFV MIP reveal cavities on the polymer surface with an average diameter of d ¼ 59 nm, which correlates well with the dimensions of CSFV particles. QCM sensor measurements yield concentration-dependent CSFV sensor responses resulting in LOD ¼ 1.7 μg/mL, LOQ ¼ 5.1 μg/mL and R 2 ¼ 0.9963. Furthermore, CSFV-MIP sensors selectively bind CSFV with selectivity factors of 2 over porcine respiratory and reproductive virus (PRRSV) and 62 over pseudorabies virus (PRV), respectively. Finally, sensor responses turned out fully reversible.

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Plant-based porcine reproductive and respiratory syndrome virus VLPs induce an immune response in mice

Cited by 12 publications

References 60 publications

Exosome-Based Vaccines: Pros and Cons in the World of Animal Health

Exosome-Based Vaccines: Pros and Cons in the World of Animal Health

Key Gaps in the Knowledge of the Porcine Respiratory Reproductive Syndrome Virus (PRRSV)

Sensing the classical swine fever virus with molecularly imprinted polymer on quartz crystal microbalance

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