Plant-Associated N2Fixation (C2H2-Reduction) by Five Rice Varieties, and Relationship with Plant Growth Characters as Affected by Straw Incorporation

Ladha, J. K.; Tirol, Agnes C.; Daroy, M. L. G.; Caldo, Gloria; Ventura, W.; Watanabe, Iwao

doi:10.1080/00380768.1986.10557484

Cited by 70 publications

(35 citation statements)

References 15 publications

(16 reference statements)

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“…However, an additional factor causing the carbon limitation on rice-associated BNF in the present study could be that the plants analyzed were in the early stages of growth (20 DAI). In field-grown rice, significant ARA was obtained only after 40 days of transplantation (36). Although IRBG500 did not show ARA in association with rice, it promoted the growth of the inoculated seedlings but led to no enhancement of their N content, suggesting that the growth promotion was probably due to mechanisms other than N 2 fixation.…”

Section: Discussionmentioning

confidence: 92%

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

et al. 2001

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Six closely related N 2 -fixing bacterial strains were isolated from surface-sterilized roots and stems of four different rice varieties. The strains were identified as Serratia marcescens by 16S rRNA gene analysis. One strain, IRBG500, chosen for further analysis showed acetylene reduction activity (ARA) only when inoculated into media containing low levels of fixed nitrogen (yeast extract). Diazotrophy of IRBG500 was confirmed by measurement of 15 N 2 incorporation and by sequence analysis of the PCR-amplified fragment of nifH. To examine its interaction with rice, strain IRBG500 was marked with gusA fused to a constitutive promoter, and the marked strain was inoculated onto rice seedlings under axenic conditions. At 3 days after inoculation, the roots showed blue staining, which was most intense at the points of lateral root emergence and at the root tip. At 6 days, the blue precipitate also appeared in the leaves and stems. More detailed studies using light and transmission electron microscopy combined with immunogold labeling confirmed that IRBG500 was endophytically established within roots, stems, and leaves. Large numbers of bacteria were observed within intercellular spaces, senescing root cortical cells, aerenchyma, and xylem vessels. They were not observed within intact host cells. Inoculation of IRBG500 resulted in a significant increase in root length and root dry weight but not in total N content of rice variety IR72. The inoculated plants showed ARA, but only when external carbon (e.g., malate, succinate, or sucrose) was added to the rooting medium.

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Section: Discussionmentioning

confidence: 92%

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

et al. 2001

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“…IR42 also displayed a high plant-associated acetylene-reducing activity (ARA) expressed per g total plant dry weight (4,10,11). OS4 exhibits a low plant-associated ARA (3, 10) and a low ability to enhance N gains (2).…”

Section: Discussionmentioning

confidence: 99%

“…Varieties or lines used here were selected on the basis of AR assays (4,10,11), N balance studies (2), and dependence on soil N as determined by the 15N uptake in plots with 15N depleted fertilizer (De Datta, IRRI, personal communication).…”

Section: Discussionmentioning

confidence: 99%

“…Rice straw was incorporated in 4 blocks, while the other 4 served as control. This experiment was likewise designed for AR assays and the details of the design and results were already published (10). Out of 5 varieties, grains of IR42 and OS4 were analyzed for 15N abundance.…”

Section: Methodsmentioning

confidence: 99%

“…Many authors have revealed differences in the stimulation of N2 fixation associated with rice plant among Oryza sativa varieties and wild Oryza species (8,(10)(11)(12)14). In all of these experiments the acetylene reduction assay (AR assay) were used.…”

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Effect of inorganic N and organic fertilizers on nitrogen-fixing (acetylene-reducing) activity associated with wetland rice plants

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Plant-Associated N₂Fixation (C₂H₂-Reduction) by Five Rice Varieties, and Relationship with Plant Growth Characters as Affected by Straw Incorporation

Cited by 70 publications

References 15 publications

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

Difference in Natural Abundance of¹⁵N in Several Rice(Oryza sativaL.) Varieties: Application for Evaluating N₂Fixation

Effect of inorganic N and organic fertilizers on nitrogen-fixing (acetylene-reducing) activity associated with wetland rice plants

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Plant-Associated N2Fixation (C2H2-Reduction) by Five Rice Varieties, and Relationship with Plant Growth Characters as Affected by Straw Incorporation

Cited by 70 publications

References 15 publications

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens

Difference in Natural Abundance of15N in Several Rice(Oryza sativaL.) Varieties: Application for Evaluating N2Fixation

Effect of inorganic N and organic fertilizers on nitrogen-fixing (acetylene-reducing) activity associated with wetland rice plants

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Plant-Associated N₂Fixation (C₂H₂-Reduction) by Five Rice Varieties, and Relationship with Plant Growth Characters as Affected by Straw Incorporation

Difference in Natural Abundance of¹⁵N in Several Rice(Oryza sativaL.) Varieties: Application for Evaluating N₂Fixation