Plant as a plenteous reserve of lectin

Ingale, Arun G.; Hivrale, A U

doi:10.4161/psb.26595

Cited by 40 publications

(18 citation statements)

References 97 publications

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“…Addition of metal chelating agent like EDTA to E. Lectin and tested for antiviral activity on HSV-I, revealed that some or all antiviral activity of E. Lectin was diminished (disappeared after addition of EDTA) indicating that the lectin was a metaloprotein that needed both Ca +2 and Mn +2 ions for its activity. Similar results ( Figure 6) were obtained by Konozy et al [39]. Also addition of EDTA diminished antiviral activity of E. Lectin when it is applied to vero cells infected with HSV-1.…”

Section: Effect Of Temperature Ph and Denaturantssupporting

confidence: 90%

Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line

Torky¹

2016

J Antivir Antiretrovir

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Lectin is a protein that specifically bound and cross-linked with carbohydrates and is involved in the activation of the lectin pathway which is considered a part of both innate and adaptive immunity. Lectin also has a role in plant defense system. In this study, lectin was isolated from Euphorbia and purified through aqueous extraction, ammonium sulfate precipitation, dialysis, filtration on superdex 75, ion exchange chromatography on SP-sepharose gel. The study showed that lectin exhibits hemagglutination activity towards rabbit erythrocytes on concentration 50 µg/ml. This hemagglutination activity was retained by lectin till 60°C. Effect of pH, and denaturalizing agent on lectin was also studied, and found to have a low pH range (6-8). Molecular weight was determined for lectin using SDS gel-electrophoresis and found to be 30 KD, and determined by gel filtration to be 60 KD, indicating that lectin is homodimer in the plant. Cytotoxicity assay of Euphorbia on the vero cell lines using MTT, was also studied and revealed that using a concentration till 100 µg/ml of Euphorbia was within the safe limit. Also the determination of antiviral activity of the Euphorbia lectin against HSV1 on vero cell lines was investigated using plaque reduction assay and MTT, showing 76% of virus inhibition. Mechanism of action of the effect of lectin on HSV1 was also investigated using MTT, and RT-PCR. The study also showed that lectin affected the virus entry and attachment phase. This inhibition of the virus was totally diminished when the lectin was combined with EDTA before infection with HSV1. Antiproliferative activity of Euphorbia was also studied against human cancer cell line by MTT assay. The gene(s) encoding the lectin was isolated by PCR, which revealed that lectin is actually encoded by multi gene family.

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Section: Effect Of Temperature Ph and Denaturantssupporting

confidence: 90%

Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line

Torky¹

2016

J Antivir Antiretrovir

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“…Also, the observed haemagglutination activity of these mucoadhesives could be due to the presence of lectins which is reported to be abundant in plants and its products. These ubiquitous highly specific sugar-binding proteins also have mitogenic, antibacterial, antifungal, antiviral as well as cytotoxic activities (Ingale and Hivrale 2013). …”

Section: Discussionmentioning

confidence: 99%

Ex-vivo evaluation of the mucoadhesive properties of Cedrela odorata and Khaya senegalensis gums with possible applications for veterinary vaccine delivery

et al. 2016

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BackgroundFew studies have investigated the interaction of bioadhesives with biologic tissues for veterinary application. Hence, this study evaluates the mucoadhesive property and vaccine delivery properties of polymers from phytogenic origin. Gums from Cedrela odorata and Khaya senegalensis were harvested, purified, dried and compressed into 500 mg tablets individually and in combined ratios. The time taken for these tablets, placed on freshly excised (5 × 5 cm) trachea and duodenal tissues of cattle, chicken, pig, sheep and goat and fastened to the basket end of a tablet dissolution machine probe set at 50 rev/min in a phosphate buffer 6.8 pH at 37 °C, to fall off the tissue was the peak adhesion time (PAT). Gum with best PAT was combined with Newcastle disease vaccine and the procedure repeated. Haemagglutination assay (HA) was conducted on the gum polymer-vaccine mix with gum and vaccine individually as controls.ResultsOn intestinal and trachea tissues, Cedrela gum polymer averagely had prolonged PAT (≈1 h 30 min and 1 h respectively) while average PAT values of Khaya gums followed the same trend but too transient PAT (≈6 and 0.3 min respectively). However on combination, Cedrela–Khaya polymer mix (1:1) was best on chicken, cattle and sheep trachea and intestinal tissues (PAT of 1 h 30 min and 2 h 24 min respectively). On combination with vaccine, the PAT of the gums reduced slightly on cattle and sheep tissues while other animal tissue showed varied results. The HA results showed the gum polymer boosted the HA property of the vaccine (Log 105), when compared to vaccine alone (Log 104).ConclusionHence, mucoadhesives from phytogenic sources have potential for non-invasive vaccine application.

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“…Lectins are widely distributed in nature and found in all kinds of organisms such as plants, fungi, animals, bacteria, and viruses. [ 1 ] The amount of lectin varies in different organisms. In the plant kingdom, they are mainly seen in the seeds of leguminous plants.…”

Section: Introductionmentioning

confidence: 99%

Use of lectins in immunohematology

Gorakshakar

Ghosh

2016

Asian J Transfus Sci

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Lectins are carbohydrate binding proteins present in seeds of many plants, especially corals and beans, in fungi and bacteria, and in animals. Apart from their hemagglutinating property, a wide range of functions have been attributed to them. Their importance in the area of immunohematology is immense. They are used to detect specific red cell antigens, to activate different types of lymphocytes, in order to resolve problems related to polyagglutination and so on. The introduction of advanced biotechnological tools generates new opportunities to exploit the properties of lectins, which were not used earlier. Stem cell research is a very important area in transplant medicine. Certain lectins detect surface markers of stem cell. Hence, they are used to understand the developmental biology of stem cells. The role of various lectins in the areas of transfusion and transplant medicine is discussed in detail in this review.

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Plant as a plenteous reserve of lectin

Cited by 40 publications

References 97 publications

Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line

Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line

Ex-vivo evaluation of the mucoadhesive properties of Cedrela odorata and Khaya senegalensis gums with possible applications for veterinary vaccine delivery

Use of lectins in immunohematology

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