Plant ABC proteins – a unified nomenclature and updated inventory

Verrier, P. J.; Bird, David A.; Burla, Bo; Dassa, Elie; Forestier, Cyrille Le; Geisler, Markus; Klein, Markus; Kolukisaoglu, Üner; Lee, Youngsook; Martinoia, Enrico; Murphy, Angus S.; Rea, Philip A.; Samuels, Lacey; Schulz, Burkhard; Spalding, Edgar P.; Yazaki, Kazufumi; Theodoulou, F. L.

doi:10.1016/j.tplants.2008.02.001

Cited by 634 publications

(644 citation statements)

References 65 publications

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“…ABC transporters can function either as importers or exporters 16 . Thus, to further investigate the direction of ABCG14-mediated transport of cytokinins, we incubated detached leaves from 12-DAG transgenic seedlings harbouring 35S::EGFP-AtABCG14 (Supplementary Fig.…”

Section: Disruption Of Atabcg14 Causes Cytokinin-deficient Phenotypesmentioning

confidence: 99%

“…ATP-binding cassette (ABC) transporters comprise one of the largest families of transport proteins, catalysing the export and import of numerous metabolites in organisms ranging from bacteria to humans 16 . The Arabidopsis genome contains more than 120 ABC transporter genes 17 , most of which encode membranebound proteins 17 .…”

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confidence: 99%

“…The Arabidopsis genome contains more than 120 ABC transporter genes 17 , most of which encode membranebound proteins 17 . ABC subfamily G (ABCG) includes 28 members of the white-brown complex half transporters, and 15 pleiotropic drug resistance full transporters 16 . Several members of this subfamily have demonstrated responsibility for exporting wax and cutin used in the formation of cuticle in aerial organs 18 , for conveying abscisic acid across the plasma membrane in leaf guard cells 19 and for exporting auxinic compounds, including indole-3-butyric acid 20 .…”

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Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins

et al. 2014

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Cytokinins are a major group of phytohormones regulating plant growth, development and stress responses. However, in contrast to the well-defined polar transport of auxins, the molecular basis of cytokinin transport is poorly understood. Here we show that an ATP-binding cassette transporter in Arabidopsis, AtABCG14, is essential for the acropetal (root to shoot) translocation of the root-synthesized cytokinins. AtABCG14 is expressed primarily in the pericycle and stelar cells of roots. Knocking out AtABCG14 strongly impairs the translocation of trans-zeatin (tZ)-type cytokinins from roots to shoots, thereby affecting the plant's growth and development. AtABCG14 localizes to the plasma membrane of transformed cells. In planta feeding of C 14 or C 13 -labelled tZ suggests that it acts as an efflux pump and its presence in the cells directly correlates with the transport of the fed cytokinin. Therefore, AtABCG14 is a transporter likely involved in the long-distance translocation of cytokinins in planta.

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Section: Disruption Of Atabcg14 Causes Cytokinin-deficient Phenotypesmentioning

confidence: 99%

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confidence: 99%

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Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins

et al. 2014

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“…2a) to isolate full-size PDR-type transporters (also known as ABC subtype G (ABCG) transporters) of P. hybrida that are abundant in phosphate-starved or mycorrhizal roots. The rationale behind the focus on these transporters, of which there are 15 in Arabidopsis 11 , 23 in Oryza sativa (rice) 11 and 23 putative factors in Solanum lycopersicum (tomato) ( Supplementary Fig. 3a), was that they are plasma membrane proteins often found in roots 12 , they are implicated in below-ground plantmicrobe interactions 13,14 , and they have affinities for compounds that are structurally related to strigolactones 8,9,15 .…”

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A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching

Kretzschmar

Kohlen

Sasse

et al. 2012

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Strigolactones were originally identified as stimulators of the germination of root-parasitic weeds 1 that pose a serious threat to resource-limited agriculture 2 . They are mostly exuded from roots and function as signalling compounds in the initiation of arbuscular mycorrhizae 3 , which are plant-fungus symbionts with a global effect on carbon and phosphate cycling 4 . Recently, strigolactones were established to be phytohormones that regulate plant shoot architecture by inhibiting the outgrowth of axillary buds 5,6 . Despite their importance, it is not known how strigolactones are transported. ATP-binding cassette (ABC) transporters, however, are known to have functions in phytohormone translocation [7][8][9] . Here we show that the Petunia hybrida ABC transporter PDR1 has a key role in regulating the development of arbuscular mycorrhizae and axillary branches, by functioning as a cellular strigolactone exporter. P. hybrida pdr1 mutants are defective in strigolactone exudation from their roots, resulting in reduced symbiotic interactions. Above ground, pdr1 mutants have an enhanced branching phenotype, which is indicative of impaired strigolactone allocation. Overexpression of Petunia axillaris PDR1 in Arabidopsis thaliana results in increased tolerance to high concentrations of a synthetic strigolactone, consistent with increased export of strigolactones from the roots. PDR1 is the first known component in strigolactone transport, providing new opportunities for investigating and manipulating strigolactone-dependent processes.Strigolactones are a new class of carotenoid-derived 10 phytohormone in land plants. In addition to their role in shoot branching, strigolactones are exuded into the rhizosphere under phosphorus-limiting conditions 5 and act as growth stimulants of arbuscular mycorrhizal fungi 3 . To identify efflux carriers of arbuscular-mycorrhiza-promoting factors such as strigolactones, we used a degenerate primer approach ( Supplementary Fig. 2a) to isolate full-size PDR-type transporters (also known as ABC subtype G (ABCG) transporters) of P. hybrida that are abundant in phosphate-starved or mycorrhizal roots. The rationale behind the focus on these transporters, of which there are 15 in Arabidopsis 11 , 23 in Oryza sativa (rice) 11 and 23 putative factors in Solanum lycopersicum (tomato) ( Supplementary Fig. 3a), was that they are plasma membrane proteins often found in roots 12 , they are implicated in below-ground plantmicrobe interactions 13,14 , and they have affinities for compounds that are structurally related to strigolactones 8,9,15 . Of six primary candidates, only P. hybrida PDR1 had increased expression in roots that were subjected to either phosphate starvation (Fig. 1a) or colonization by the arbuscular mycorrhizal fungus Glomus intraradices (Fig. 1b). Furthermore, PDR1 transcript levels increased in response to treatment with the synthetic strigolactone analogue GR24 or the auxin analogue 1-naphthaleneacetic acid (NAA) (Fig. 1c). Auxin has been shown to upregulate strigolactone-bi...

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“…Plant ABC transporters are implicated in tasks as diverse as cellular detoxification of organic toxins and heavy metals, resistance against pathogens, transport of long chain fatty acids and of phytohormones, and regulation of the stomatal aperture 7,8 . In our attempts to elucidate physiological roles of hitherto undescribed ABC transporters, we noted that AtABCB14 9 (also known as AtPGP14 7 or AtMDR12 8 ) was strongly expressed in guard cells. Other ABC transporters, i.e.…”

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The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2

et al. 2008

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Carbon dioxide uptake and water vapour release in plants occur through stomata, which are formed by guard cells. These cells respond to light intensity, CO2 and water availability, and plant hormones. The predicted increase in the atmospheric concentration of CO2 is expected to have a profound effect on our ecosystem. However, many aspects of CO2-dependent stomatal movements are still not understood. Here we show that the ABC transporter AtABCB14 modulates stomatal closure on transition to elevated CO2. Stomatal closure induced by high CO2 levels was accelerated in plants lacking AtABCB14. Apoplastic malate has been suggested to be one of the factors mediating the stomatal response to CO2 (Refs 4,5) and indeed, exogenously applied malate induced a similar AtABCB14-dependent response as high CO2 levels. In isolated epidermal strips that contained only guard cells, malate-dependent stomatal closure was faster in plants lacking the AtABCB14 and slower in AtABCB14-overexpressing plants, than in wild-type plants, indicating that AtABCB14 catalyses the transport of malate from the apoplast into guard cells. Indeed, when AtABCB14 was heterologously expressed in Escherichia coli and HeLa cells, increases in malate transport activity were observed. We therefore suggest that AtABCB14 modulates stomatal movement by transporting malate from the apoplast into guard cells, thereby increasing their osmotic pressure. University of Science and Technology, Pohang, Korea; [10][11][12] and had a strongly reduced sensitivity to glibenclamide, ABA, calcium and auxin, which are well known to control stomatal movement. We therefore were interested whether AtABCB14 also exhibits a regulatory function in guard cell physiology.AtABCB14 expression, as visualized by the activity of an AtABCB14 promoter::GUS fusion construct, is not restricted to guard cells of leaves only, but is also found in guard cells of stems, flowers and siliques ( Fig. 1a-f). In leaves, GUS activity was also detected in epidermal and at very low levels in mesophyll cells (Fig. 1c). These promoter::GUS expressions corresponded to the transcript levels detected in mesophyll and guard cell protoplasts (Fig. 1g). Transient expression of an 35S::AtABCB14:GFP construct in Arabidopsis protoplasts revealed that AtABCB14 is targeted to the plasma membrane (Fig. 1h, i). AtABCB14:sGFP expressed under the control of the AtABCB14 native promoter was targeted to the plasma membrane of guard cells (Fig. 1n). Coexpression of AtABCB14 with AtAHA2:RFP, a fusion protein of a plasma membrane localized proton pump with a red fluorescent protein 13 , resulted in a perfect co-localization ( Fig. 1j-l). Fractionation of microsomes on a sucrose density gradient further confirmed that AtABCB14:HA protein was targeted to the plasma membrane: the distribution pattern of the protein crossreacting with the HA antibody corresponded to that of AtPDR8, a plasma membrane protein 14 and differed from the patterns of the ER (Bip) and vacuolar markers (γ-TIP) (Fig. 1m). These results indica...

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Plant ABC proteins – a unified nomenclature and updated inventory

Cited by 634 publications

References 65 publications

Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins

Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins

A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching

The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2

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