Planotetraspora thailandica sp. nov., isolated from soil in Thailand

Abstract

A Gram-positive-staining, filamentous bacterial strain that developed cylindrical sporangia containing four oval-to rod-shaped spores at the ends of short sporangiophores on branched aerial mycelium was isolated from tropical rainforest soil near a hot spring. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; the whole-cell hydrolysate contained rhamnose, madurose, glucose, galactose and 3-Omethylmannose as whole-cell sugars. The predominant menaquinone was MK-9(H 4 ). Mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were iso-C 16 : 0 and 10-methylated C 17 : 0 . The G+C content of the DNA was 71

Planomonospora corallina sp. nov., isolated from soil

“…from the organic layer on the soil surface and kept at 4 C. The sample was dried at room temperature for 10 days, then dry-heated at 100 C for 1 h. The dried sample was treated using the flooding method of Suzuki (2001). The organism was isolated on soil extract agar (Suriyachadkun et al, 2009) supplemented with 25 mg nalidixic acid l À1 , 50 mg cycloheximide l À1 and 1 mg terbinafine l À1 , then incubated at 28 C for 30 days. The isolate was purified on soil extract agar.…”

“…The pure culture was preserved in glycerol (10 %, v/v) at À80 C and in a liquid-dried form for longterm storage. Type strains of every species of the genus Planomonospora were used in this study for comparison of physiological, morphological and chemotaxonomic properties and DNA-DNA hybridization.Morphological characteristics of mycelia and sporangia of strain A-T 11038 T were determined by light microscope (model CX 31; Olympus) with a 50Â long working distance objective lens (model SLMPLN50X; Olympus) and scanning electron microscope (model JSM-5410 LV; JEOL) using cultures grown on soil extract agar (Suriyachadkun et al, 2009) at 28 C for 14-21 days. Cultures for scanning electron microscopy were prepared as described by Itoh et al (1989).…”

“…Morphological characteristics of mycelia and sporangia of strain A-T 11038 T were determined by light microscope (model CX 31; Olympus) with a 50Â long working distance objective lens (model SLMPLN50X; Olympus) and scanning electron microscope (model JSM-5410 LV; JEOL) using cultures grown on soil extract agar (Suriyachadkun et al, 2009) at 28 C for 14-21 days. Cultures for scanning electron microscopy were prepared as described by Itoh et al (1989).…”

Asanoa endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Boesenbergia rotunda

“…Morphological characteristics of strain BR3-1 T grown on modified soil extract agar (Suriyachadkun et al, 2009) were observed using light and scanning electron microscopy (model JSM-5410 LV; JEOL). The preparation of scanning electron microscopic samples was as described previously (Thawai et al, 2005).…”

Actinoplanes siamensis sp. nov., isolated from soil

“…Morphological characteristics of strain A-T 6646 T grown on modified soil extract agar (Suriyachadkun et al, 2009) at 28 u C for 7-14 days were observed with a light microscope (model CX 31; Olympus) with a 640 long working distance objective lens (model LUCPLFLN40XRC; Olympus) and a scanning electron microscope (model JSM-5410 LV; JEOL). Samples on modified soil extract agar for scanning electron microscopy were prepared as described by Itoh et al (1989).…”