Planar chromatographic screening and quantification of coumarin in food, confirmed by mass spectrometry

Krüger, Stephanie; Winheim, Luisa; Morlock, Gertrud E.

doi:10.1016/j.foodchem.2017.07.058

Cited by 35 publications

(28 citation statements)

References 15 publications

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“…Chromatography was performed on HPTLC plates, silica gel 60, with a mixture of n -hexane, ethyl acetate, and ammonia (3.8:1.3:0.05, v/v/v) up to a migration distance of 55 mm. 30 The addition of that small volume of ammonia as a modifier resulted in an optimal sharpness of the coumarin band at hR F 50 ± 2. Despite the minimal sample preparation, a baseline separation from matrix components was evident ( Figure S-4 ) and a fast development time of less than 15 min was reached.…”

Section: Resultsmentioning

confidence: 96%

“…The single quadrupole MS determination showed a substantially poorer sensitivity with a LOD of 500 ng band –1 for sample and standard zones, when compared with fluorescence measurement. However, the use of high-resolution MS (HRMS) 30 or MS–MS will improve the LOD.…”

Section: Resultsmentioning

confidence: 99%

“…A currently developed high-performance thin-layer chromatography (HPTLC) method for the determination of coumarin in different food products used postchromatographic derivatization with a methanolic potassium solution. 30 Immersing the HPTLC plate in the alkaline solution led to an opening of the lactone ring of coumarin 31 and resulted in an intensive fluorescence (excitation wavelength at 360 nm), which allowed a sensitive determination and quantification of coumarin in the food samples.…”

Section: Introductionmentioning

confidence: 99%

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Bioprofiling of Cosmetics with Focus on Streamlined Coumarin Analysis

2017

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Facing the widespread use of cosmetic products in daily use and recognizing the very limited information obtained by target analysis, a method suited for comprehensive characterization of cosmetics was aimed at. The biological activity of ingredients of 20 cosmetics taken from 16 different product groups and their coumarin contents were investigated via chromatography linked to bioassays (direct bioautography) and mass spectrometry. It allows for screening a large number of cosmetic products within a short time to generate a more valid database on their coumarin content and their contribution to the overall exposure. Bioactivity profiling of cosmetics with regard to bioactive ingredients opens new avenues for a comprehensive characterization of important substances in products of daily use, helpful for the legally required safety and risk assessment of cosmetic products, especially for multiple product usage. As for coumarin, a ubiquitary fragrance compound of allergenic potential, which is under recurrent discussion due to its hepatoxic properties, it is necessary to be able to estimate the regular intake via cosmetics for a valid risk assessment. This newly developed bioprofiling method allowed a selective determination of coumarin down to 1.3 mg kg–1, even for very matrix-rich cosmetics despite minimalism in sample preparation. The declaration limits according to European Cosmetics Regulation were completely covered. Mean coumarin contents of 20 cosmetic products reached up to 2218 mg kg–1. The repeatabilities (%RSD, n = 3) were between 1.1 and 2.9%, and the mean recoveries (n = 5) were between 96 and 102% for the different cosmetic matrices.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioprofiling of Cosmetics with Focus on Streamlined Coumarin Analysis

2017

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“…Together, Cs, FCs, and PMFs constitute the so‐called OHCs, which are the main components of the non‐volatile fraction of Citrus essential oils. The current regulations focused on Cs in foods only limit the content of Cs, whereas PMFs are known for their lipid‐lowering, hypoglycaemic, anti‐inflammatory, and anticancer activities, and as such their use as nutraceuticals is constantly increasingly.…”

Section: Introductionmentioning

confidence: 99%

“…Many mono‐dimensional and two‐dimensional liquid chromatographic methods coupled to highly sensitive detectors have been developed for the analysis of OHCs, but to date high‐performance liquid chromatography equipped with photodiode array detector (HPLC‐PDA) is the main technique employed for FC determination in routine analysis . As a result of a ring‐test validation approach, the Analytical Working Group of International Fragrance Association (IFRA) have concluded that the quantification of 15 selected FC analytes, based on HPLC‐UV, cannot be achieved below 10 mg L −1 , however …”

Section: Introductionmentioning

confidence: 99%

Oxygen heterocyclic compound screening in Citrus essential oils by linear retention index approach applied to liquid chromatography coupled to photodiode array detector

Arigò

Rigano

Micalizzi

et al. 2019

Flavour & Fragrance J

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To date, liquid chromatography coupled to a photodiode array (PDA) detector is the main technique employed to analyse furocoumarins (FCs) in essential oils. This type of detection may lead to poor performance because of the low sensitivity and selectivity of PDA detection. In this work, the linear retention index (LRI) was used in combination with a UV‐Vis library for the identification of 35 oxygen heterocyclic compounds in Citrus essential oils, making it possible to distinguish compounds with very similar spectra. As opposed to retention time, which can significantly change with the instrument or laboratory used, LRI represents a kind of normalized relative retention time, as independent as possible from operating conditions. From a qualitative point of view, reproducibility results are provided to definitively assess the suitability of such an approach for inter‐laboratory use and for a fully automated process, even when pure and very expensive standard materials are not available, as just the LRI database and the UV‐Vis library are needed. From a quantitative point of view, calibration curves were created in pure solvent and by adding standard compounds to several blank matrices, with the aim of evaluating differences, overcoming matrix effects, achieving the correct limits of quantification, and providing the quantitative profile of real samples. The LRI system is not listed in the Commission Decision 2002/657/EC as criteria for the Full Scan UV‐Vis detection; however, its validity and applicability are here investigated and proposed for future applications, in combination with the UV‐Vis library.

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Analysis of coumarin in food and plant tissue without extraction based on voltammetry of microparticles

Chen

Zhao

et al. 2021

Food Measure

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Planar chromatographic screening and quantification of coumarin in food, confirmed by mass spectrometry

Cited by 35 publications

References 15 publications

Bioprofiling of Cosmetics with Focus on Streamlined Coumarin Analysis

Bioprofiling of Cosmetics with Focus on Streamlined Coumarin Analysis

Oxygen heterocyclic compound screening in Citrus essential oils by linear retention index approach applied to liquid chromatography coupled to photodiode array detector

Analysis of coumarin in food and plant tissue without extraction based on voltammetry of microparticles

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