Placental telomere length decline with gestational age differs by sex and TERT, DNMT1, and DNMT3A DNA methylation

Wilson, Samantha L.; Liu, Yao; Robinson, Wendy P.

doi:10.1016/j.placenta.2016.10.001

Cited by 31 publications

(28 citation statements)

References 41 publications

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“…TL has been found to be associated with epigenetic alterations across different populations and environmental exposures [22][23][24]. TL was not only associated with the global methylations among young adults [23], but also associated with specific DNA methylation changes in response to gestational age among placental samples and in response to smoking exposure among older adults [22,24], respectively. Given that, we hypothesized that TL might be associated with the MS and hence the MS might be able to reflect the aging process which the TL is involved in.…”

Section: Introductionmentioning

confidence: 93%

Leukocyte telomere length and epigenetic-based mortality risk score: associations with all-cause mortality among older adults

et al. 2018

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Telomere length (TL) has been established as a biomarker of aging and aging-related health outcomes, but showed only a weak or inconsistent association with all-cause mortality in previous epidemiological studies. Recently, an epigenetic 'mortality risk score' (MS) based on whole blood DNA methylation at 10 mortality-related CpG sites has been demonstrated to be strongly related to all-cause mortality at the population level. This study aimed to address the association between TL and this MS, and to assess and compare their associations with all-cause mortality. The MS was derived from the DNA methylation profiles measured by Illumina Human Methylation450K Beadchip and TL was measured by quantitative PCR at baseline among 1517 participants aged 50-75 of the German ESTHER cohort study. In cross-sectional bi- and multivariable analyses, the MS was strongly associated and showed monotonic dose-response relationships with TL (p-values <0.05). However, only the MS but not TL was associated with all-cause mortality during a median follow-up of 12.5 years. After controlling for potential covariates and TL, hazard ratios (95% CI) for all-cause mortality for low, moderate and high levels of the MS defined by 1, 2-5 and >5 CpG sites with aberrant methylation were 2.24 (1.13-4.41), 3.31 (1.76-6.22) and 6.33 (3.22-12.41) compared to a MS of 0, respectively. Our investigation shows that the epigenetic-based MS is strongly associated with TL, a broadly accepted aging biomarker, and at the same time shows much stronger associations with all-cause mortality than the latter.

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Section: Introductionmentioning

confidence: 93%

Leukocyte telomere length and epigenetic-based mortality risk score: associations with all-cause mortality among older adults

et al. 2018

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“…Telomere length analysis based on isolated DNA TRF [29][30][31] STELA [32][33][34] Q-PCR, T/S ratio [29,[35][36][37][38] Telomere length analysis based on examination of interphase nuclei…”

Section: Methods Referencesmentioning

confidence: 99%

“…RT-PCR for hTERC [29] Telomerase activity determination TRAP [33,38,48,52,53] TRAP in situ [52,53] Genome-wide technologies Illumina methylation array [37] Interphase Q-FISH, first described in 1998 by de Pauw and colleagues [63], is applicable on nondividing cells. It compares the fluorescent signals obtained after hybridization with a telomere-specific probe and with a probe targeting a single copy gene.…”

Section: Methods Referencesmentioning

confidence: 99%

“…Wilson et al [37] used array technology (Illumina Infinium Human Methylation 450 BeadChip) to study methylation alterations in genes hTERT, DNMT1, and DNMT3A in human placentas. MALDI-TOF technology was employed to determine the level of DNA methylation of hTERC in placenta using Sequenom EpiTyper platform [29].…”

Section: Methods Examining Epigenetic Changes and Chromatin Structurementioning

confidence: 99%

“…Some authors took into consideration that the position of villous tissue in the placenta may influence the telomere length, and therefore collected and processed samples of the whole placenta from more locations [29,37,84]; nevertheless, no site-specific differences of telomere length were determined except [84] showing that the telomerase activity was detected in term placenta restricted to biopsy sites near umbilical cord only. Moreover, the Southern blot-based TRF assay discovered longer telomere length in placental samples than in cord blood cells [84].…”

Section: Placenta In Normal Pregnancymentioning

confidence: 99%

See 2 more Smart Citations

Telomeres and Telomerase Activity in the Human Placenta

Jirkovská¹,

Korabečná²,

Laššáková³

2020

Telomerase and Non-Telomerase Mechanisms of Telomere Maintenance

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Placenta is a transient organ ensuring the intrauterine development of the individual. To meet fetal requirements, rapid and continuous cell proliferation enlarges the areas of tissues maintaining maternofetal transport. The cell division in placenta is accompanied with shortening of telomeres leading to cell senescence. Telomerase activity, on the other hand, ensures replication of telomeres and allows the organ to serve till the end of pregnancy. This balanced process may be negatively influenced by unfavorable circumstances. Here, we summarize available data on telomere length as well as telomerase activity in placentas from normal and complicated pregnancies; attention is also paid to the comparison of methods used in relevant studies.

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Accessing the human trophoblast stem cell state from pluripotent and somatic cells

Karvas

David

Theunissen

2022

Cell. Mol. Life Sci.

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Trophoblasts are specialized epithelial cells that perform critical functions during blastocyst implantation and mediate maternal–fetal communication during pregnancy. However, our understanding of human trophoblast biology remains limited since access to first-trimester placental tissue is scarce, especially between the first and fourth weeks of development. Moreover, animal models inadequately recapitulate unique aspects of human placental physiology. In the mouse system, the isolation of self-renewing trophoblast stem cells has provided a valuable in vitro model system of placental development, but the derivation of analogous human trophoblast stem cells (hTSCs) has remained elusive until recently. Building on a landmark study reporting the isolation of bona fide hTSCs from blastocysts and first-trimester placental tissues in 2018, several groups have developed methods to derive hTSCs from pluripotent and somatic cell sources. Here we review the biological and molecular properties that define authentic hTSCs, the trophoblast potential of distinct pluripotent states, and methods for inducing hTSCs in somatic cells by direct reprogramming. The generation of hTSCs from pluripotent and somatic cells presents exciting opportunities to elucidate the molecular mechanisms of human placental development and the etiology of pregnancy-related diseases.

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Placental telomere length decline with gestational age differs by sex and TERT, DNMT1, and DNMT3A DNA methylation

Cited by 31 publications

References 41 publications

Leukocyte telomere length and epigenetic-based mortality risk score: associations with all-cause mortality among older adults

Leukocyte telomere length and epigenetic-based mortality risk score: associations with all-cause mortality among older adults

Telomeres and Telomerase Activity in the Human Placenta

Accessing the human trophoblast stem cell state from pluripotent and somatic cells

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