Placental microRNA expression in pregnancies complicated by preeclampsia

Abstract

Objective-The role of post-transcription regulation in preeclampsia is largely unknown. We investigated preeclampsia related placental microRNA (miRNA) expression using microarray and confirmatory qRT-PCR experiments.Study design-Placental expressions of characterized and novel miRNAs (1,295 probes) were measured in samples collected from 20 preeclampsia cases and 20 controls. Differential expression was evaluated using Students T-test and fold change analyses. In pathway analysis, we examined functions/functional relationships of targets of differentially expressed miRNAs.Results-Eight miRNAs were differentially expressed (1 up-and 7 down-regulated) among preeclampsia cases compared with controls. These included previously identified candidates (miR-210, miR-1 and a miRNA in the 14q32.31 cluster region) and others that are novel (miR-584 and miR-34c-5p). These miRNAs target genes that participate in organ/system development (cardiovascular and reproductive system), immunologic dysfunction, cell adhesion, cell cycle and signaling.Conclusion-Expression of microRNAs that target genes in diverse pathophysiological processes is altered in the setting of preeclampsia.

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

“…Hu et al (2009) performed a highthroughput miRNA microarray study and revealed significantly increased levels of 7 miRNAs (miR-16, miR-29b, miR-195, miR-26b, miR-181a, miR-335, and miR-222) in placentas derived from pregnancies with severe PE, occurring after 34 weeks, compared with those derived from healthy controls. (Zhu et al, 2009;Enquobahrie et al, 2011;Mayor-Lynn et al, 2011;Zhang et al, 2011). Enquobahrie et al (2011) further confirmed the downregulation of previously identified miRNA candidates (miR-1, miR-328, miR-139-5p, miR-500, and miR-1247) and found two novel miRNA candidates to be downregulated in pregnancies complicated by PE (miR-584 and miR-34c-5p).…”

“…(Zhu et al, 2009;Enquobahrie et al, 2011;Mayor-Lynn et al, 2011;Zhang et al, 2011). Enquobahrie et al (2011) further confirmed the downregulation of previously identified miRNA candidates (miR-1, miR-328, miR-139-5p, miR-500, and miR-1247) and found two novel miRNA candidates to be downregulated in pregnancies complicated by PE (miR-584 and miR-34c-5p). Noack et al (2011) identified another six miRNAs to be overexpressed in five cases with severe PE who delivered at gestational age ranging from 29 to 37 weeks (let-7b, miR-302*, miR-104, miR-128a, miR-182*, and miR-133b).…”

Expression and role of microRNA 18b and hypoxia inducible factor‑1α in placental tissues of preeclampsia patients

“…Previous studies suggest that miRNA have important roles in various basic physiological processes, such as cell growth, differentiation, proliferation and apoptosis (5)(6)(7). Furthermore, previous studies have indicated a series of expression imbalances between miRNA and target genes in PE placental tissues, which have resulted in cell dysfunction in placental tissues; thus, abnormal expression levels of miRNA have been demonstrated to be closely related to the occurrence of PE in PE patients (8)(9)(10). miR18b (miR-18b) is involved in the regulation of multiple diseases.…”

Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations

“…Although different hypotheses have been put forth, the underlying etiology of preeclampsia is unknown (32). Analysis of miRNAs from formalin-fixed placental specimens of preeclampsia patients has demonstrated altered miRNA profiles (33)(34)(35). Deep sequencing of miRNAs obtained from placental tissues suggests that, in the setting of preeclampsia, the isomiR profile is altered with increased 3′ end addition of an adenosine (36).…”