Placental Expression of Cytokeratin 18 and Serum Levels of Tissue Polypeptide Antigen in Women with Pregnancy-Induced Hypertension

Bancher‐Todesca, Dagmar; Hefler, Lukas; Zeisler, Harald; Schatten, Christian; Husslein, Peter; Heinze, Georg; Tempfer, Clemens

doi:10.3109/10641950109152645

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…In chorionic villi of human placentae, E-cadherin was observed not at the apical surface of the STB layer in contact with the intervillous space, which in situ is occupied by maternal blood, but in the basal membrane of the STB in contact with remnants of the CTB layer and the chorionic mesenchyma (Figure S1a). In this and most of the subsequent images, the trophoblast layer was identified with cytokeratin 18, a canonical marker of epithelial cells [28]. The intensity of the fluorescent signal of E-cadherin in the STB layer was not affected in placentae derived from ZIKV-infected women (Figure S1b).…”

Section: Resultsmentioning

confidence: 91%

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability

Miranda

Martín-Tapia

Valdespino-Vázquez

et al. 2019

Cells

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The cytotrophoblast of human placenta transitions into an outer multinucleated syncytiotrophoblast (STB) layer that covers chorionic villi which are in contact with maternal blood in the intervillous space. During pregnancy, the Zika virus (ZIKV) poses a serious prenatal threat. STB cells are resistant to ZIKV infections, yet placental cells within the mesenchyme of chorionic villi are targets of ZIKV infection. We seek to determine whether ZIKV can open the paracellular pathway of STB cells. This route is regulated by tight junctions (TJs) which are present in the uppermost portion of the lateral membranes of STB cells. We analyzed the paracellular permeability and expression of E-cadherin, occludin, JAMs –B and –C, claudins -1, -3, -4, -5 and -7, and ZO-1, and ZO-2 in the STB of placentae from ZIKV-infected and non-infected women. In ZIKV-infected placentae, the pattern of expression of TJ proteins was preserved, but the amount of claudin-4 diminished. Placentae from ZIKV-infected women were permeable to ruthenium red, and had chorionic villi with a higher mean diameter and Hofbauer hyperplasia. Finally, ZIKV added to the basolateral surface of a trophoblast cell line reduced the transepithelial electrical resistance. These results suggest that ZIKV can open the paracellular pathway of STB cells.

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Section: Resultsmentioning

confidence: 91%

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability

Miranda

Martín-Tapia

Valdespino-Vázquez

et al. 2019

Cells

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Ultrastructural and Immunofluorescence Studies of Placental Tissue in Hypertensive Diseases of Pregnancy

Judson¹,

Nadarajah²,

Nalliah³

et al. 2010

Research J. of Biological Sciences

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Placental Expression of Cytokeratin 18 and Serum Levels of Tissue Polypeptide Antigen in Women with Pregnancy-Induced Hypertension

Abstract: Cytokeratin 18 is overexpressed in the syncytiotrophoblast of women with PIH. Serum levels of TPA are elevated among women with PIH and correlate with disease severity and low fetal birth weight.

Cited by 2 publications

References 12 publications

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability

Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability

Ultrastructural and Immunofluorescence Studies of Placental Tissue in Hypertensive Diseases of Pregnancy

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