Placenta as a reservoir of stem cells: an underutilized resource?

Pipino, Caterina; Shangaris, Panicos; Resca, Elisa; Zia, Silvia; Deprest, Jan; Sebire, Neil J.; David, Anna L.; Guillot, Pascale V.; Coppi, Paolo De

doi:10.1093/bmb/lds033

Cited by 81 publications

(95 citation statements)

References 121 publications

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“…It contains cells, of fetal and/or amniotic membrane origin, which express stemness markers. Amniotic fluid cells are a heterogeneous population of epithelial origin derived from all three germ layers, its composition depending on the gestational period [1,2].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Phenotypic Characterization of Human Amniotic Fluid-Derived Cells: A Morphological and Proteomic Approach

Pipino

PierdomenicoLaura

TomoPamela

et al. 2015

Stem Cells and Development

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Mesenchymal Stem Cells derived from Amniotic Fluid (AFMSCs) are multipotent cells of great interest for regenerative medicine. Two predominant cell types, that is, Epithelial-like (E-like) and Fibroblast-like (F-like), have been previously detected in the amniotic fluid (AF). In this study, we examined the AF from 12 donors and observed the prevalence of the E-like phenotype in 5, whereas the F-like morphology was predominant in 7 samples. These phenotypes showed slight differences in membrane markers, with higher CD90 and lower Sox2 and SSEA-4 expression in F-like than in E-like cells; whereas CD326 was expressed only in the E-like phenotype. They did not show any significant differences in osteogenic, adipogenic or chondrogenic differentiation. Proteomic analysis revealed that samples with a predominant E-like phenotype (HC1) showed a different profile than those with a predominant F-like phenotype (HC2). Twenty-five and eighteen protein spots were differentially expressed in HC1 and HC2 classes, respectively. Of these, 17 from HC1 and 4 from HC2 were identified by mass spectrometry. Protein-interaction networks for both phenotypes showed strong interactions between specific AFMSC proteins and molecular chaperones, such as preproteasomes and mature proteasomes, both of which are important for cell cycle regulation and apoptosis. Collectively, our results provide evidence that, regardless of differences in protein profiling, the prevalence of E-like or F-like cells in AF does not affect the differentiation capacity of AFMSC preparations. This may be valuable information with a view to the therapeutic use of AFMSCs.

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Section: Introductionmentioning

confidence: 99%

Molecular and Phenotypic Characterization of Human Amniotic Fluid-Derived Cells: A Morphological and Proteomic Approach

Pipino

PierdomenicoLaura

TomoPamela

et al. 2015

Stem Cells and Development

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“…46,47 The human placenta is a complex organ uniquely composed of trophoblast, immune (eg, Hofbauer), mesenchymal (eg, fibroblast), endothelial cells, 48 and hematopoietic stem cells 49 ; each performing different critical functions throughout pregnancy. These include invasion of the decidua, 50 maternal and villous angiogenesis, 51 decidual immunity, 52 maternal and fetal endocrine regulation, 53 and nutrient/ waste transport.…”

Section: Circrnasmentioning

confidence: 99%

Placental transcriptome in development and pathology: expression, function, and methods of analysis

Cox¹,

Leavey²,

Nosi³

et al. 2015

American Journal of Obstetrics and Gynecology

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“…Described bio-objects can be used to produce stem cells [14,20], in an experiment, testing of pharmaceuticals [10], auto-banking [17], for laboratory studies [7,9]. Fetal membranes are used as scaffolds for constructions in tissue engineering [15,16], allografts in reconstructive surgery [11,18] and ophthalmology [13,6].…”

Section: Institute For Problems Of Cryobiology and Cryomedicine Of Thmentioning

confidence: 99%

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Prokopіuk¹,

Prokopyuk²,

Мусатова³

et al. 2015

JCOT

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There have been studied morphological safety and functional state of the explants of human placenta, umbilical cord and fetal membranes by vital staining techniques using the MTT and resazurin reduction tests, level of glucose in incubation medium, activity of lactate dehydrogenase and alkaline phosphatase before and after cryopreservation. It has been found that proposed program of cryopreservation allows keeping a high level of viability of the explants of placenta, umbilical cord and fetal membranes, the most informative methods of assessing the safety of these biological objects before and after cryopreservation are method of vital staining, determination of glucose content in incubation medium, MTT-test and resazurin reduction test.

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Placenta as a reservoir of stem cells: an underutilized resource?

Abstract: Understanding the role of placenta stem cells during pregnancy and their paracrine actions could help in the study of some diseases that affect the placenta during pregnancy.

Cited by 81 publications

References 121 publications

Molecular and Phenotypic Characterization of Human Amniotic Fluid-Derived Cells: A Morphological and Proteomic Approach

Molecular and Phenotypic Characterization of Human Amniotic Fluid-Derived Cells: A Morphological and Proteomic Approach

Placental transcriptome in development and pathology: expression, function, and methods of analysis

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

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