Pivotal roles of Tomato photoreceptor SlUVR8 in seedling development and UV-B stress tolerance

Liu, Xiaorui; Zhang, Qianwen; Yang, Guoqian; Zhang, Chunli; Dong, Huaxi; Liu, Yang; Yin, Ruohe; Li, Lin

doi:10.1016/j.bbrc.2019.11.073

Cited by 39 publications

(19 citation statements)

References 34 publications

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“…UVR8 is widely conserved across the green lineage, and several studies have confirmed its role in mediating UV-B responses in a diverse range of species (Allorent et al, 2016;Tilbrook et al, 2016;Clayton et al, 2018;Soriano et al, 2018;Han et al, 2019;Kondou et al, 2019;Tokutsu et al, 2019;Liu et al, 2020). G101 is universally conserved in UVR8 orthologs, as are residues involved in photoreception and dimer stability (Rizzini et al, 2011;Fernandez et al, 2016;Han et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

Podolec

Lau

Wagnon

et al. 2020

Preprint

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The plant UV-B photoreceptor UVR8 plays an important role in UV-B acclimation and survival. UV-B absorption by homodimeric UVR8 induces its monomerization and interaction with the E3 ubiquitin ligase COP1, leading ultimately to gene expression changes. UVR8 is inactivated through redimerization, facilitated by RUP1 and RUP2. Here, we describe a novel semi-dominant, hyperactive allele, namely uvr8-17D, that harbors a glycine-101 to serine mutation. UVR8G101S-overexpression led to weak constitutive photomorphogenesis and extreme UV-B responsiveness. UVR8G101S was observed to be predominantly monomeric in vivo and, once activated by UV-B, was not efficiently inactivated. Analysis of a UVR8G101S crystal structure revealed the distortion of a loop region normally involved in stabilization of the UVR8 homodimer. Plants expressing a UVR8 variant combining G101S with the previously described W285A mutation exhibited robust constitutive photomorphogenesis. This work provides further insight into UVR8 activation and inactivation mechanisms, and describes a genetic tool for the manipulation of photomorphogenic responses.

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Section: Discussionmentioning

confidence: 99%

A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

Podolec

Lau

Wagnon

et al. 2020

Preprint

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“…Plates were then stratified at 4°C in darkness for 2 d before being moved into a standard growth chamber under constant white light. UV‐B light treatments were conducted with conditions modified from (Liu et al 2019). Narrow band UV‐B lamps (Philips TL20W/01RS; 1.5 μmol/m 2 /s) were used to activate UVR8‐mediated physiological responses.…”

Section: Methodsmentioning

confidence: 99%

“…Plants sense and respond to light signals via several types of photoreceptors. The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically regulates UV‐B photomorphogenesis, including seedling de‐etiolation, leaf development, stomatal movements, stress tolerance, floral transition, phototropism and lateral root development (Wargent et al 2009; Demkura and Ballare 2012; Li et al 2013; Tossi et al 2014; Vandenbussche et al 2014; Jenkins 2017; Yin and Ulm 2017; Arongaus et al 2018; Liang et al 2019; Liu et al 2019; Vanhaelewyn et al 2019). In addition, UVR8 mediates acclimation to low‐fluence UV‐B light and promotes tolerance to high‐fluence UV‐B stress (Kliebenstein et al 2002; Brown et al 2005; Favory et al 2009).…”

Section: Introductionmentioning

confidence: 99%

The C‐terminal 17 amino acids of the photoreceptor UVR8 is involved in the fine‐tuning of UV‐B signaling

Dong

Yang

et al. 2020

JIPB

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Plant UV‐B responses are mediated by the photoreceptor UV RESISTANCE LOCUS 8 (UVR8). In response to UV‐B irradiation, UVR8 homodimers dissociate into monomers that bind to the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). The interaction of the C27 domain in the C‐terminal tail of UVR8 with the WD40 domain of COP1 is critical for UV‐B signaling. However, the function of the last 17 amino acids (C17) of the C‐terminus of UVR8, which are adjacent to C27, is unknown, although they are largely conserved in land plants. In this study, we established that Arabidopsis thaliana UVR8 C17 binds to full‐length UVR8, but not to COP1, and reduces COP1 binding to the remaining portion of UVR8, including C27. We hypothesized that overexpression of C17 in a wild‐type background would have a dominant negative effect on UVR8 activity; however, C17 overexpression caused strong silencing of endogenous UVR8, precluding a detailed analysis. We therefore generated YFP‐UVR8N423 transgenic lines, in which C17 was deleted, to examine C17 function indirectly. YFP‐UVR8N423 was more active than YFP‐UVR8, suggesting that C17 inhibits UV‐B signaling by attenuating binding between C27 and COP1. Our study reveals an inhibitory role for UVR8 C17 in fine‐tuning UVR8–COP1 interactions during UV‐B signaling.

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“…Therefore, a possible interaction between phyB and a specific UV photoreceptor could be suggested; for example, UVR8 interacted with the central regulator of light signalling COP1, since both proteins accumulate in the nucleus under UV exposure in Arabidopsis (Rizzini et al, 2011), which resulted in a reduction in hypocotyl length (Favory et al, 2009). Moreover, UVR8 activity in the promotion of UV tolerance was largely reported in different species, mainly through induction of flavonoids and anthocyanin synthesis in Arabidopsis (Kliebenstein, Lim, Landry, & Last, 2002; Favory et al, 2009) and tomato (Liu et al, 2020). In addition, both UVR8 and phyB regulate COP1 activity.…”

Section: High Light Stressmentioning

confidence: 99%

Phytochrome type B family: The abiotic stress responses signaller in plants

Silva

D’Amico-Damião

Carvalho

2020

Annals of Applied Biology

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Photoreceptors are primarily known as key photomorphogenic modulators of various physiological events during plant development. Although there are different groups of photoreceptors, the phytochrome B (phyB) family mediates developmental responses in a wide range of plant species, from seed germination to flowering. In addition, these molecules also regulate abiotic stress acclimation responses, such as salinity, drought, low/high temperature, high light and heavy metals. The signalling pathways mediated by phyB could enhance plant resistance to environmental stresses, as phyB photoreceptors reduce leaf transpiration, increase the antioxidant system, enhance protective pigments and increase the expression of genes related to plant stress acclimation. Thus, the elucidation of positive or negative roles for phyB in these stress tolerance characteristics would provide essential knowledge for genetic engineering, improving plant growth and development in critical environments. In this review, we cover the main findings on how the phyB family works to modulate abiotic stress by discussing biochemical and molecular aspects of the underlying mechanisms operated by these photoreceptors.

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Pivotal roles of Tomato photoreceptor SlUVR8 in seedling development and UV-B stress tolerance

Cited by 39 publications

References 34 publications

A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

A constitutively monomeric UVR8 photoreceptor allele confers enhanced UV-B photomorphogenesis

The C‐terminal 17 amino acids of the photoreceptor UVR8 is involved in the fine‐tuning of UV‐B signaling

Phytochrome type B family: The abiotic stress responses signaller in plants

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