Pituitary Tumors Associated with Acromegaly1

Young, Donald G.; Bahn, Robert C.; Randall, Raymond V.

doi:10.1210/jcem-25-2-249

Cited by 42 publications

(6 citation statements)

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“…The high secretion rate of HGH required to maintain such a high plasma level was accompanied by a relatively low storage capacity within the tumour. The contrast between chromophobe tumours of this type and the purely eosinophilic tumours rich in HGH lends support to the suggestion of Young et al (1965) that there may be two fundamentally different types of pituitary tumour causing acromegaly. …”

supporting

confidence: 60%

“…and the non-functioning chromophobe tumours (0-012/tg./mg.). The tumours causing acromegaly did not include a purely eosinophilic tumour, which may be very rich in HGH (Young et al 1965 ;Lloyd & Meares, 1965). The non-functioning chromophobe tumours, containing minute concentrations of HGH, showed occasional cells with light eosinophilic granulation.…”

mentioning

confidence: 93%

“…and the non-functioning chromophobe tumours (0-012/tg./mg.). The tumours causing acromegaly did not include a purely eosinophilic tumour, which may be very rich in HGH (Young et al 1965 ;Lloyd & Meares, 1965 extremely high plasma HGH levels and a relatively low tumour concentration. The tumour was not very large and the acromegaly was moderately severe, progressive and of about 3 yr. duration.…”

mentioning

confidence: 99%

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Growth Hormone Concentration in Human Pituitary Tumours

Lloyd¹,

Donald²,

Catt³

et al. 1969

Journal of Endocrinology

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inspection of starch-gel electrophoresis patterns. Histological examination of the tumour tissue was performed on paraffin sections of formalin-fixed specimens after staining with haematoxylin and eosin, eosin and methyl blue, and PAS-orange G. Extracts of pituitary tissue were prepared by homogenization in tris-EDTA-boric acid buffer at pH 8\m=.\9.The clear supernatant obtained after centrifugation was subjected to radioimmunoassay and to starch-gel electrophoresis. Radioimmunoassay was performed by the solid-phase method (Catt, Niall & Tregear, 1967) using Wilhelmi HGH 705 A as the assay standard. Extracts were diluted to within the range of the assay (0-1-5 ng. HGH) and estimations based on the mean values obtained from two dilutions parallel to the standard curve. Electrophoresis was performed in a vertical tray holding five 0-3 ml. samples of extract in buried-origin slots, each equivalent to 50 mg. of the original fresh tissue. After electrophoresis at 280 for 5 hr., the gels were stained with amidoblack and nigrosine. The HGH content of each extract was assessed visually on a scale of 0 to 5 +, 5 + being equivalent to the main band in the normal pituitary pattern. This method is based on the demonstra¬ tion that HGH forms a major component of the electrophoretic pattern of human pituitary extracts (Ferguson & Wallace, 1963).The results (Table 1) show a general agreement between visual estimation and radioimmunoassay and a marked variation between the mean HGH concentrations in the normal anterior pituitary glands (23/ig./mg.), the tumours causing acromegaly (4-5/tg./mg.) and the non-functioning chromophobe tumours (0-012/tg./mg.). The tumours causing acromegaly did not include a purely eosinophilic tumour, which may be very rich in HGH (Young et al. 1965 ;Lloyd & Meares, 1965). The non-functioning chromophobe tumours, containing minute concentrations of HGH, showed occasional cells with light eosinophilic granulation. The 37-yr.-old acromegalie patient had

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supporting

confidence: 60%

mentioning

confidence: 93%

“…and the non-functioning chromophobe tumours (0-012/tg./mg.). The tumours causing acromegaly did not include a purely eosinophilic tumour, which may be very rich in HGH (Young et al 1965 ;Lloyd & Meares, 1965 extremely high plasma HGH levels and a relatively low tumour concentration. The tumour was not very large and the acromegaly was moderately severe, progressive and of about 3 yr. duration.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Growth Hormone Concentration in Human Pituitary Tumours

Lloyd¹,

Donald²,

Catt³

et al. 1969

Journal of Endocrinology

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“…Our much smaller series of 18 cases did not show this correlation, possibly because it included five patients with pituitary fossa area within normal limits and only one patient with mean plasma GH greater than 100 ng/ml. The very wide range of concentration of GH in pituitary tumour tissue in acromegaly (Young, Bahn, and Randall, 1965;Lloyd et al, 1969) comprises part of the evidence put forward by Young et al (1965) that qualitatively different types of tumour may cause this disease. A given tumour may be heterogeneous in cell type and function but the existence of strongly eosinophilic tumours rich in GH and chromophobe tumours with very low GH concentrations, both causing acromegaly, is reasonably well established.…”

Section: Discussionmentioning

confidence: 99%

Radioimmunoassay of human growth hormone: technique and application to plasma, cerebrospinal fluid, and pituitary extracts

Thomas¹,

Lloyd²,

Thomas³

1972

J Clin Pathol

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“…As in cases reported by others (14,16), the microscopic appearance of the present tumor was benign despite the invasive potential of the tumor. Histology indicated a chromophobe adenoma even though clinical acromegaly with an elevated growth hormone level was present (32). The growth hormone level was very strikingly elevated in this case, and the tumor grew exceptionally vigorously in tissue culture with continued production of growth hormone.…”

Section: Discussionmentioning

confidence: 68%