Pituitary Gonadotropins, Prolactin and Growth Hormone Differentially Regulate AQP1 Expression in the Porcine Ovarian Follicular Cells

Skowroński, Mariusz T.; Młotkowska, Patrycja; Tanski, Damian; Lepiarczyk, Ewa; Oklinski, Michal Krystian Egelund; Nielsén, Sören; Skowrońska, Agnieszka

doi:10.3390/ijms19010005

Cited by 16 publications

(20 citation statements)

References 62 publications

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“…It was widely thought that granulosa cells (GCs) supported the development and maturation of follicles though the complex interactions [ 5 , 6 ], and that the growth and proliferation of GCs play critical roles in the biological processes of recruitment, selection, atresia, ovulation and luteolysis of follicles [ 7 , 8 ]. Previous studies have found that steroids, growth factors, and cytokine factors secreted by GCs are essential for the survival and growth of follicles [ 9 , 10 ], and moreover, the high apoptosis of GCs can impair folliculogenesis and result in the increased follicular atresia [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

C/EBPβ Promotes STAT3 Expression and Affects Cell Apoptosis and Proliferation in Porcine Ovarian Granulosa Cells

Yuan

Zhou

et al. 2018

Genes

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Previous studies suggest that signal transducer and activator of transcription 3 (STAT3) and CCAAT/enhancer binding protein beta (C/EBPβ) play an essential role in ovarian granulosa cells (GCs) for mammalian follicular development. Several C/EBPβ putative binding sites were previously predicted on the STAT3 promoter in mammals. However, the molecular regulation of C/EBPβ on STAT3 and their effects on cell proliferation and apoptosis remain virtually unexplored in GCs. Using porcine GCs as a model, the 5′-deletion, luciferase report assay, mutation, chromatin immunoprecipitation, Annexin-V/PI staining and EdU assays were applied to investigate the molecular mechanism for C/EBPβ regulating the expression of STAT3 and their effects on the cell proliferation and apoptosis ability. We found that over and interfering with the expression of C/EBPβ significantly increased and decreased the messenger RNA (mRNA) and protein levels of STAT3, respectively. The dual luciferase reporter assay showed that C/EBPβ directly bound at −1397/−1387 of STAT3 to positively regulate the mRNA and protein expressions of STAT3. Both C/EBPβ and STAT3 were observed to inhibit cell apoptosis and promote cell proliferation. Furthermore, C/EBPβ might enhance the antiapoptotic and pro-proliferative effects of STAT3. These results would be of great insight in further exploring the molecular mechanism of C/EBPβ and STAT3 on the function of GCs and the development of ovarian follicles in mammals.

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Section: Introductionmentioning

confidence: 99%

C/EBPβ Promotes STAT3 Expression and Affects Cell Apoptosis and Proliferation in Porcine Ovarian Granulosa Cells

Yuan

Zhou

et al. 2018

Genes

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“…In previous research [28] we found a stimulatory effect of FSH and LH on AQP1 mRNA and protein expression in the Gc ant Tc of pig medium and large ovarian follicles and co-cultures of these cells. In the present research, AQP5 was mainly submitted to the regulatory effect of FSH and LH, since these hormones markedly increased AQP5 mRNA and protein expression in Gc and Tc, as well as in co-cultures of the cells.…”

Section: Discussionmentioning

confidence: 76%

“…Inspiration for this study came from the earlier results obtained from the pig [28]. The study found that follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) regulated AQP1 expression in the ovarian follicular cells of pigs.…”

Section: Discussionmentioning

confidence: 99%

“…We recently demonstrated a stimulatory effect of PRL on AQP1 protein expression in the co-cultures of Gc and Ts of medium and large follicles [28]. GH increased the expression of AQP1 protein in Gc isolated from large follicles.…”

Section: Discussionmentioning

confidence: 99%

“…We observed the specific pattern of expression and established an anatomical basis for examined AQPs, which indicates that they play important functions in the regulation of water movement to maintain adequate hydration and the regeneration ability of the reproductive tract. Recently, our study found that the follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) regulated AQP1 expression in the ovarian follicular cells of pigs [28].…”

Section: Introductionmentioning

confidence: 99%

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Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells

Skowroński

Młotkowska

Tanski

et al. 2019

IJMS

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This study aimed to examine the effect of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) on Aquaporin 5 (AQP5) expression in granulosa (Gc) and theca cells (Tc) from medium (MF) and large (LF) ovarian follicles of pigs. The results showed that GH significantly decreased the expression of AQP5 in Gc from MF in relation to the control. In the Gc of large follicles, PRL stimulated the expression of AQP5. However, the increased expression of AQP5 in the Tc of LF was indicated by GH and PRL in relation to the control. A significantly higher expression of the AQP5 protein in the Gc from MF and LF was indicated by FSH and PRL. In co-cultures, an increased expression of AQP5 was observed in the Gc from LF incubated with LH, PRL, and GH. A significantly increased expression of AQP5 was also observed in co-cultures of Tc from all type of follicles incubated with LH, whereas PRL stimulated the expression of AQP5 in Tc from MF. Moreover, AQP5 protein expression increased in the co-culture isolated from MF and LF after treatment with FSH, LH, PRL, and GH. AQP5 immunoreactivity was observed in the cytoplasm, mainly in the perinuclear region and endosomes, as well as in the cell membranes of Gc and Tc from the LF and MF.

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MicroRNA miR‐125b can suppress ovarian granulosa cell functions: Interrelationships with FSH

Fabová

Loncová

Sirotkin

2022

Cell Biochemistry & Function

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This study aimed to evaluate the involvement of miR-125b and its interrelationship with follicle-stimulating hormone (FSH) in the control of basic ovarian granulosa cell functions. The effect of miR-125b mimics on basic functions of porcine ovarian granulosa cells cultured with and without FSH, and the effect of FSH on the expression of endogenous miR-125b was examined. Expression levels of miR-125b, viability, proliferation (accumulation of PCNA and cyclin B1), apoptosis (accumulation of bax and caspase 3), the accumulation of FSH receptors (FSHR), steroid hormones, insulin-like growth factor I (IGF-I), oxytocin, and prostaglandin E2 release were analysed by reverse transcription-quantitative polymerase chain reaction, Trypan blue exclusion test, quantitative immunocytochemistry, and ELISA. Transfection of cells with miR-125b mimics inhibited cell viability, proliferation, apoptosis, the occurrence of FSHR, progesterone, testosterone, estradiol, and oxytocin release but stimulated prostaglandin E2 output. FSH promoted cell viability, proliferation, steroid hormones, IGF-I, oxytocin, and prostaglandin E2 output and reduced the expression of miR-125b and apoptosis. Furthermore, miR-125b mimics supported the effect of FSH on the release of estradiol, IGF-I, and prostaglandin E2, and inverted FSH influence on cell viability, proliferation, apoptosis, progesterone, and testosterone output. FSH supported both inhibitory and stimulatory action of miR-125b on ovarian cell functions. Present observations indicate that: miR-125b can be involved in the control of basic ovarian functions and that miR-125b and FSH are antagonists in their actions on ovarian cell functions. The ability of FSH to reduce miR-125b expression and the ability of miR-125b mimics to decrease the occurrence of FSHR and to modify FSH effects indicate the existence of the self-inhibiting FSH-miR-125b axis and that miR-125b can mediate the actions of FSH on ovarian cells.

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Pituitary Gonadotropins, Prolactin and Growth Hormone Differentially Regulate AQP1 Expression in the Porcine Ovarian Follicular Cells

Cited by 16 publications

References 62 publications

C/EBPβ Promotes STAT3 Expression and Affects Cell Apoptosis and Proliferation in Porcine Ovarian Granulosa Cells

C/EBPβ Promotes STAT3 Expression and Affects Cell Apoptosis and Proliferation in Porcine Ovarian Granulosa Cells

Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells

MicroRNA miR‐125b can suppress ovarian granulosa cell functions: Interrelationships with FSH

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