Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells

Skowroński, Mariusz T.; Młotkowska, Patrycja; Tanski, Damian; Lepiarczyk, Ewa; Kempisty, Bartosz; Jaśkiewicz, Łukasz; Pareek, Chandra Shekhar; Skowrońska, Agnieszka

doi:10.3390/ijms20194914

Cited by 7 publications

(6 citation statements)

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“…The present study aims to investigate the causes of the decreased oocyte competence during the NBS in buffalo. Many biological processes are required for developmental competence, with the exchange of information between oocyte and follicular environment promoting oocyte maturation 26 . Therefore, the focus of this study was to evaluate differences in the miRNA and transcriptomic profiles of OOs and corresponding FCs from buffalo ovaries collected in the BS and NBS.…”

Section: Discussionmentioning

confidence: 99%

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Capra

Lazzari

Russo

et al. 2020

Sci Rep

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Season clearly influences oocyte competence in buffalo (Bubalus bubalis); however, changes in the oocyte molecular status in relation to season are poorly understood. This study characterizes the microRNA (miRNA) and transcriptomic profiles of oocytes (OOs) and corresponding follicular cells (FCs) from buffalo ovaries collected in the breeding (BS) and non-breeding (NBS) seasons. In the BS, cleavage and blastocyst rates are significantly higher compared to NBS. Thirteen miRNAs and two mRNAs showed differential expression (DE) in FCs between BS and NBS. DE-miRNAs target gene analysis uncovered pathways associated with transforming growth factor β (TGFβ) and circadian clock photoperiod. Oocytes cluster in function of season for their miRNA content, showing 13 DE-miRNAs between BS and NBS. Between the two seasons, 22 differentially expressed genes were also observed. Gene Ontology (GO) analysis of miRNA target genes and differentially expressed genes (DEGs) in OOs highlights pathways related to triglyceride and sterol biosynthesis and storage. Co-expression analysis of miRNAs and mRNAs revealed a positive correlation between miR-296-3p and genes related to metabolism and hormone regulation. In conclusion, season significantly affects female fertility in buffalo and impacts on oocyte transcriptomic of genes related to folliculogenesis and acquisition of oocyte competence. Water buffalo (Bubalus bubalis) is an important livestock resource for both developing and developed countries. The major factor affecting buffalo farming profitability is reproductive seasonality, resulting in cycles of calving and milk production. Buffalo is a short-day breeder, with increased fertility in response to decreasing day length 1,2. This photoperiod-dependent seasonality pattern is more pronounced as distance from the equator, together with variations in the light/dark ratio, increases. In Italy, in order to satisfy market demand, out of breeding mating strategy (OBMS), consisting in interrupting sexual promiscuity or the use of artificial insemination (AI) during the breeding season (BS), is commonly utilized 2. The OBMS improves the distribution of calving throughout the year, but it reduces fertility 3. Longer post-partum anoestrus periods as well as higher incidence of embryonic mortality are observed in months with increasing daylight length and particularly in midwinter , which coincides with the transition to seasonal anoestrus at Italian latitudes 1,4. The embryonic mortality is due to inadequate luteal growth and function, resulting in reduced progesterone secretion 5. This has a negative impact on embryo growth, associated with alterations in transcriptomic and proteomic profiles of the embryos and chorioamnios/caruncles 6,7 , which ultimately impair embryo attachment to the uterine endometrium.

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Section: Discussionmentioning

confidence: 99%

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Capra

Lazzari

Russo

et al. 2020

Sci Rep

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“…Beside E2 and P4, prolactin (PRL) plays a major role in fluid production in the oviduct [22]. We recently found that PRL increased AQP5 mRNA and protein expression in the granulosa (GC) and theca cells (TC) obtained from medium (MF) and large follicles (LF) and in cocultures of these cells [23]. Prolactin had a stimulatory effect on AQP1 protein expression in the coculture of the abovementioned cells [23].…”

Section: Discussionmentioning

confidence: 99%

“…We recently found that PRL increased AQP5 mRNA and protein expression in the granulosa (GC) and theca cells (TC) obtained from medium (MF) and large follicles (LF) and in cocultures of these cells [23]. Prolactin had a stimulatory effect on AQP1 protein expression in the coculture of the abovementioned cells [23]. Moreover, we indicated the functional AQPs expression in these cells using swelling assay with an AQPs blocker, because a significant increase in the cell volume in hypotonic conditions treated with PRL was demonstrated when compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

“…In most species of mammals, the oocyte matures within the ovarian follicle and during the ovulation period it is mainly in the stage of metaphase II (MII) meiosis. In light of studies on animals, it is assumed that an important role in these mechanisms can be played by connexins (Cxs) [32][33][34] and aquaporins (AQPs) [8,19,23,35]. Gap junctions appearing in oocyte-cumulus oophorus cell complexes are composed of protein Cxs [36].…”

Section: Discussionmentioning

confidence: 99%

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Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

Tanski

Skowrońska

Eliszewski

et al. 2020

IJMS

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Aquaporins (AQPs) are a group of small, integral membrane proteins which play an important role in fluid homeostasis in the reproductive system. In our previous study, we demonstrated AQP1, 5 and 9 protein expression and localization in the porcine oviduct. The presence of these isoforms could suggest their role in the transport of the ovum to the uterus by influencing the epithelial cells’ production of oviductal fluid. The aim of this study was to evaluate the expression of AQP1, AQP5 and AQP9 in the infundibulum, ampulla and isthmus in the porcine oviduct during the estrous cycle (early luteal phase, days 2–4, medium luteal phase, days 10–12, late luteal phase days 14–16, follicular phase days 18–20) and pregnancy (period before implantation, days 14–16 and after the implantation, days 30–32) using the Real-Time PCR technique. As clearly demonstrated for the first time, AQP1, 5, and 9 gene expression is influenced by the estrus cycle and pregnancy. Furthermore, expression of AQPs in the porcine oviduct may provide the physiological medium that sustains and enhances fertilization and early cleavage-stage embryonic development. Overall, our study provides a characterization of oviduct AQPs, increasing our understanding of fluid homeostasis in the porcine oviduct to successfully establish and maintain pregnancy.

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“…The development of mammalian follicles is a key process in the ovary, and the proliferation and growth of GCs directly promotes follicular development [18]. As they proliferate and differentiate during follicular development, GCs can synthesize a variety of hormones and growth factors, and they express their receptors [19,20]. These substances regulate the growth, differentiation and maturation of follicular inner membrane cells and oocytes through gap junctions, and then, they regulate follicular development [21].…”

Section: Introductionmentioning

confidence: 99%

FGF21 Promotes Proliferation and Estradiol Synthesis in Porcine Granulosa Cells

Zhou

Shi

et al. 2021

Preprint

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Background: The proliferation and estradiol synthesis in granulosa cells (GCs) directly promotes follicular development. Previous studies had found that FGF21 regulated the hypothalamic-pituitary-gonad axis in response to the control of fertility. However, the functions and mechanisms of FGF21 in GCs are unclear.Results: Here, we found that the mRNA and protein levels of FGF21 in the ovarian tissue of high-yielding sows (Sus scrofa) was higher than that in low-yielding sows. Moreover, FGF21 was predominantly expressed in porcine GCs. Additionally, ELISA assay showed estradiol was significantly increased when overexpression of FGF21 in porcine GCs. Meanwhile, overexpressed FGF21 up-regulated both the mRNA and protein levels of key estradiol synthesis-related genes in porcine GCs, including StAR, CYP11A1 and CYP19A1. Corresponsingly, knockdown FGF21 inhibited estradiol levels and its synthesis-related genes expression. Besides, overexpression of FGF21 promoted the proliferation of porcine GCs, displayed as increasing the percentage of S-phase cells in cell cycle and EdU positive cells, including cell viability, and upregulated cell cycle genes, including cell cycle protein B (Cyclin B) and protein E (Cyclin E). Corresponsingly, knockdown FGF21 in porcine GCs suppressed the cell cycle and cell viability, as well as EdU positive cell number.Conclusions: These findings highlight that FGF21 is associated with the development of GCs and may be a novel underlying regulator of porcine follicular development.

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Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells

Cited by 7 publications

References 50 publications

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Seasonal effects on miRNA and transcriptomic profile of oocytes and follicular cells in buffalo (Bubalus bubalis)

Changes in Aquaporin 1, 5 and 9 Gene Expression in the Porcine Oviduct According to Estrous Cycle and Early Pregnancy

FGF21 Promotes Proliferation and Estradiol Synthesis in Porcine Granulosa Cells

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