Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Signalling Enhances Osteogenesis in UMR-106 Cell Line

Juhász, Tamás; Matta, Csaba; Katona, Éva; Somogyi, Csilla; Takács, Roland; Hajdú, Tibor; Helgadóttir, Sólveig; Fodor, János; Csernoch, László; Tóth, Gábor; Bakó, Éva; Reglődi, Dóra; Tamás, Andrea; Zákány, Róza

doi:10.1007/s12031-014-0389-1

Cited by 32 publications

(53 citation statements)

References 87 publications

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“…Additionally, intracellular Ca 2+ concentration can be elevated by PACAP [ 100 ] or VIP [ 101 ], resulting in an activation of classical PKCs and ERK both infl uencing osteoblast differentiation [ 102 ]. Nonetheless, in UMR-106 cell line we were not able to detect signifi cant Ca 2+ concentration change and no signifi cant alteration was detected in activation of classical PKCs such as PKCα [ 14 ] (Fig. 20.2 ).…”

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 84%

“…Besides this dynamic alteration of intracellular Ca-homeostasis, PACAP also exerts effects on matrix mineralization. We have reported that the inorganic matrix components of UMR-106 cell line can be elevated by PACAP addition [ 14 ]. Moreover, an altered mineralization was detected during tooth formation of PACAP defi cient mice [ 10 ], suggesting a yet unknown connection between PACAP and mineralized tissue formation.…”

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 92%

“…20.2 ). Interestingly, the phosphorylation level of CREB transcription factors was not signifi cantly increased [ 14 ], raising the possibility of the participation of MAPK signalling cascade as an alternative signalling pathway for the Runx2 regulation [ 93 ].…”

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 94%

“…Indeed, the expression of BMP6 and 7 elevated in the presence of PACAP in UMR-106 cells indicating a strong positive effect of the neuropeptide in bone differentiation. Moreover, as a sign of BMPR activation, a pronounced elevation of the nuclear presence of Smad1 transcription factor was detected under the effect of PACAP administration [ 14 ]. TGFβ/BMP signalling pathways are involved in the regulation and activation of VIP signalling cascades.…”

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 97%

“…TGFβ/BMP signalling pathways are involved in the regulation and activation of VIP signalling cascades. Exogenous VIP may also result in the activation of Smads [ 95 ] suggesting a complex signalling with numerous alternative routes during bone development [ 14 ].…”

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 99%

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Role of PACAP and VIP Signalling in Regulation of Chondrogenesis and Osteogenesis

Juhász

Tamás

Zákány

et al. 2016

Current Topics in Neurotoxicity

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Pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) are multifunctional proteins that can regulate diverse physiological processes. These are also regarded as neurotrophic and antiinfl ammatory substances in the CNS, and PACAP is reported to prevent harmful effects of oxidative stress. In the last decade more and more data accumulated on the similar function of PACAP in various tissues, but its cartilage-and bone-related presence and functions have not been widely investigated yet. In this summary we plan to verify the presence and function of PACAP and VIP signalling tool kit during cartilage differentiation and bone formation. We give evidence about the protective function of PACAP in cartilage regeneration with oxidative or mechanically stress and also with the modulation of PACAP signalling in vitro in osteogenic cells. Our observations imply the therapeutic perspective that PACAP might be applicable as a natural agent exerting protecting effect during joint infl ammation and/or may promote cartilage regeneration during degenerative diseases of articular cartilage.

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Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 84%

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 92%

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 94%

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 97%

Section: Bone Formation Under the Control Of Vip And Pacap Signallingmentioning

confidence: 99%

See 3 more Smart Citations

Role of PACAP and VIP Signalling in Regulation of Chondrogenesis and Osteogenesis

Juhász

Tamás

Zákány

et al. 2016

Current Topics in Neurotoxicity

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Peripheral Neuronal Control of Bone Remodeling

Motyl

Barbe

2018

Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism

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Short Anabolic Peptides for Bone Growth

Amso

Cornish²,

Brimble

2016

Medicinal Research Reviews

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Loss of bone occurs in the age-related skeletal disorder, osteoporosis, leading to bone fragility and increased incidence of fractures, which are associated with enormous costs and substantial morbidity and mortality. Recent data indicate that osteoporotic fractures are more common than other diseases, which usually attract public attention (e.g., heart attack and breast cancer). The prevention and treatment of this skeletal disorder are therefore of paramount importance. Majority of osteoporosis medications restore skeletal balance by reducing osteoclastic activity, thereby reducing bone resorption. These agents, however, do not regenerate damaged bone tissue, leaving limited options for patients once bone loss has occurred. Recently, attention has turned to bone-anabolic agents. Such agents have the ability to increase bone mass and strength, potentially reversing structural damage. To date, only one bone-anabolic drug is available in the market. The discovery of more novel, cost-effective bone anabolic agents is therefore a priority to treat those suffering from this disabling condition. Short peptides offer an important alternative for the development of novel bone-anabolic agents given their high target binding specificity, which translates into potent activity with limited side effects. This review summarizes attempts in the identification of bone-anabolic peptides, and their development for promoting bone growth.

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Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Signalling Enhances Osteogenesis in UMR-106 Cell Line

Cited by 32 publications

References 87 publications

Role of PACAP and VIP Signalling in Regulation of Chondrogenesis and Osteogenesis

Role of PACAP and VIP Signalling in Regulation of Chondrogenesis and Osteogenesis

Peripheral Neuronal Control of Bone Remodeling

Short Anabolic Peptides for Bone Growth

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