Pitfalls in assay and interpretation of serum gammaglutamyl transpeptidase activity

Martin, Jennifer V.; Gray, Peter B.; Goldberg, David M.

doi:10.1016/0009-8981(75)90402-7

Cited by 11 publications

(1 citation statement)

References 7 publications

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“…Aspirates were diluted with an equal volume of glycerol, stored at -20 "C for up to 6 months, and the following enzyme determinations were performed: trypsin and Chymotrypsin by pH-stat techniques [14]; GGT by a continuous spectrophotometric method [15]; ALP [16], and 5NT [17] by colorimetric tech niques. The first two enzymes were assayed as milli gram by comparison with appropriate standards, and the last three, as units of activity (U = 1 pmole X M X min-1 at 37 °C).…”

Section: Introductionmentioning

confidence: 99%

Activities of y-G lutarmi Transferase, 5'-Nucleotidase and Alkaline Phosphatase in Human Duodenal Aspirate

Jm¹,

Dm²

1986

Enzyme

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Duodenal aspirates were obtained before, during, and after stimulation with secretin-cholecystokinin in 26 patients whose pancreatic function was classified as normal, moderately reduced, or severely reduced. The activities of γ-glutamyl transferase (GGT), alkaline phosphatase (ALP), and 5'-nucleotidase (5NT) in the aggregated duodenal aspirate collected 10—40 min after stimulation showed marked overlap between the functional groups and lacked diagnostic value. For all three enzymes, the peak response occurred later in the severely impaired group than in those with normal pancreatic function. The three enzymes showed significant positive correlations with each other, and were negatively correlated with the output of trypsin and Chymotrypsin and, in contrast with these proteolytic enzymes which were reduced in pancreatic disease, GGT, ALP, and 5NT all tended to increase with pancreatic disease. Contrary to a previous report, GGT did not serve as a useful index of pancreatic cancer in this study.

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Section: Introductionmentioning

confidence: 99%