Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide‑induced pulmonary inflammation by inhibiting interleukin‑8 production and NF‑κB activation

Abstract

Pistacia weinmannifolia (PW) has been used in traditional Chinese medicine to treat headaches, dysentery, enteritis and influenza. However, PW has not been known for treating respiratory inflammatory diseases, including chronic obstructive pulmonary disease (COPD). The present in vitro analysis confirmed that PW root extract (PWRE) exerts anti-inflammatory effects in phorbol myristate acetate- or tumor necrosis factor α (TNF-α)-stimulated human lung epithelial NCI-H292 cells by attenuating the expression of interleukin (IL)-8, IL-6 and Mucin A5 (MUC5AC), which are closely associated with the pulmonary inflammatory response in the pathogenesis of COPD. Thus, the aim of the present study was to evaluate the protective effect of PWRE on pulmonary inflammation induced by cigarette smoke (CS) and lipopoly-saccharide (LPS). Treatment with PWRE significantly reduced the quantity of neutrophils and the levels of inflammatory molecules and toxic molecules, including tumor TNF-α, IL-6, IL-8, monocyte chemoattractant protein-1, neutrophil elastase and reactive oxygen species, in the bronchoalveolar lavage fluid of mice with CS- and LPS-induced pulmonary inflammation. PWRE also attenuated the influx of inflammatory cells in the lung tissues. Furthermore, PWRE downregulated the activation of nuclear factor-κB and the expression of phosphodiesterase 4 in the lung tissues. Therefore, these findings suggest that PWRE may be a valuable adjuvant treatment for COPD.

Pistacia weinmannifolia root exerts a protective role in ovalbumin‑induced lung inflammation in a mouse allergic asthma model

“…Pistacia weinmannifolia (PW) is used as a herbal medicine in China (16,17) and its major metabolites possess biological activities, such as inhibitory activities against histamine release (16,18,19). In a previous study, it was confirmed that the anti-inflammatory activities of P. weinmannifolia root extract (PWRE) in PMA/tumour necrosis factor-α-stimulated airway epithelial cells and in pulmonary inflammatory response induced by cigarette smoke and lipopolysaccharide (LPS) (20). Based on these results and those of other studies (16-20), which reflect the anti-inflammatory activities of PWRE on pulmonary inflammation, it was hypothesized that PWRE could exert a protective effect against ovalbumin (OVA)-induced lung inflammation.…”

“…In a previous study, it was confirmed that the anti-inflammatory activities of P. weinmannifolia root extract (PWRE) in PMA/tumour necrosis factor-α-stimulated airway epithelial cells and in pulmonary inflammatory response induced by cigarette smoke and lipopolysaccharide (LPS) (20). Based on these results and those of other studies (16-20), which reflect the anti-inflammatory activities of PWRE on pulmonary inflammation, it was hypothesized that PWRE could exert a protective effect against ovalbumin (OVA)-induced lung inflammation. Therefore, the aim of the present study was to evaluate the regulatory effects of PWRE against eosinophil recruitment and Th2 cytokines, IgE and mucus overproduction, which are the major characteristics of allergic asthma.…”

“…PWRE was prepared as previously described (20). P. weinmannifolia roots (PWRs) were collected from the Yunnan province of China.…”

“…A voucher specimen recorded as D180305001 was deposited at the International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology. The active substance of PWR was extracted by the processing method described in the International Conference on Harmonisation and Ministry of Food and Drug Safety guidelines (20). The collected roots were dried immediately following sampling and then ground to a powder.…”

Effect of Qinbai Qingfei Concentrated Pellets on substance P and neutral endopeptidase of rats with post-infectious cough

“…Traditional Chinese medicine (TCM) has been demonstrated to exert a pivotal role in the treatment of infectious diseases. For instance, Pistacia weinmannifolia J. Poisson ex Franch has been reported to effectively suppress the pulmonary inflammation stimulated by cigarette smoke (CS) and lipopolysaccharide (LPS) [ 7 ]. Schisandra sphenanthera Rehd.…”