Piperlongumine attenuates IL-1β-induced inflammatory response in chondrocytes

Abstract

Piperlongumine (PLM) is a natural product from the pods of a plant call Piper longum and was reported to possess antibacterial, antiangiogenic, neuroprotective, anti-inflammatory and anti-tumor activities. However, the role of PLM in inflammatory responses in human Osteoarthritis (OA) chondrocytes has not been yet explored. Thus, in this study, we investigated the anti-inflammatory action of PLM in human OA chondrocytes. Our results demonstrated that PLM treatment effectively reversed IL-1β-inhibited cell viability in a dose-dependent manner. In addition, PLM significantly inhibited the production of NO and PGE2, iNOS and COX-2 expression, as well as suppressed the production of MMP-3 and MMP-13 in IL-1β-stimulated human OA chondrocytes. Furthermore, PLM significantly prevented IL-1β-induced NF-κB activation in human OA chondrocytes. In conclusion, these findings demonstrated that PLM attenuated inflammatory responses in human OA chondrocytes stimulated by IL-1β, possibly through the NF-κB signaling pathway. Thus, PLM may serve as a potential anti-inflammatory agent in the treatment of OA.

RNA-Seq Reveals Protective Mechanisms of Mongolian Medicine Molor-Dabos-4 on Acute Indomethacin-Induced Gastric Ulcers in Rats

“…Other natural compounds include ZOR (ginger) extracts, whose active constituents have been shown to possess antioxidant, anti-inflammatory and antitumor properties in in vitro and in vivo studies [ 40 , 41 ]. In addition, a previous study reported the antibacterial and antioxidant effects of TCR [ 42 ] while Hu et al discovered that PLL could not only exert anti-inflammatory effects against human osteoarthritis chondrocytes but also reverse the inhibitory effects of IL-1β on cell viability [ 43 ]. Although only 0.72% inclusion of 6-shagaol was found in MD-4, it has been reported that 6-shagaol is the main active compound of ginger and exerted significant anti-inflammatory and antioxidant actions that promoted mucosal repair of ulcerated rats [ 44 ].…”

Chondroprotection and Molecular Mechanism of Action of Phytonutraceuticals on Osteoarthritis

“…Piperlongumine (PLM) contained in the Piper longum pods effectively modifies the viability of cells inhibited by IL-1β in a dose-dependent manner [ 110 ]. PLM significantly suppresses the NO and PGE2 production, iNOS and COX-2 expression, as well as the MMP-3 and MMP-13 production, and the NF-κB activation stimulated by IL-1β in human OA chondrocytes.…”

“…PLM significantly suppresses the NO and PGE2 production, iNOS and COX-2 expression, as well as the MMP-3 and MMP-13 production, and the NF-κB activation stimulated by IL-1β in human OA chondrocytes. Moreover, piperlongumine attenuates the inflammatory responses in human OA chondrocytes stimulated by IL-1β, possibly through the NF-κB signaling pathway and, for this reason, can serve as a potential anti-inflammatory agent in the OA treatment [ 110 ].…”