Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

Abstract

Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H(2)O(2))-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H(2)O(2) (0-1000 micromol/L) and it was observed that 180 micromol/L H(2)O(2) reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H(2)O(2)-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 microg/ml) of three different extracts (aqueous, methanol and hexane) of PS. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H(2)O(2)-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H(2)O(2)-induced HUVECs.

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

“…The endothelial cells lining the blood vessels are very sensitive to injury caused by oxidative stress. Damage to endothelial cell structure and function contributes to blood vessel diseases, including atherosclerosis, thrombosis and vasculitis (Hafizah et al, 2010). Additionally, ROS activate transcriptional messengers, such as NF-κB and activator protein-1 (AP-1), both of which upregulate the production of inflammatory cytokines and mediators.…”

“…Therefore, human umbilical vein endothelial cells (HUVEC) are commonly used to study vascular dysfunction (Hafizah et al, 2010). Further, hydrogen peroxide (H2O2) is a principal mediator of ROS-dependent signaling, and exposure of endothelial cells to H2O2 induces the activation of ROS-dependent signaling cascades (Eligini et al, 2009).…”

“…Further, hydrogen peroxide (H2O2) is a principal mediator of ROS-dependent signaling, and exposure of endothelial cells to H2O2 induces the activation of ROS-dependent signaling cascades (Eligini et al, 2009). Incubation of HUVEC with 100 μmol/L H2O2 has been shown to induce oxidative stress, without inducing apoptosis (Lin et al, 2011), by inhibiting endogenous antioxidant activity and activating inflammatory signaling pathways (Hafizah et al, 2010).…”

A review of the literature and latest advances in research ofPiper sarmentosum

“…Aqueous and ethanol extracts of root, stem, leave and fruit have been investigated for antioxidant activity using two in vitro models, β-carotene linoleate model and DPPH model, and these studies have shown that ethanol extracts of leaves and fruit are having good antioxidant activity (Hussain et al, 2009a). Aqueous extracts (10% w/v) of the plant have also exhibited ferric reducing antioxidant power due to their high phenolic contents (Hafizah et al, 2010).…”

“…Ex vivo models: Aqueous, methanol and hexane extracts of leaves in a concentration range 100, 150, 250 and 300 μg/mL have shown preservation of the levels of malondehyde, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidise (GPx) in human umbilical vascular endothelial cells (HUVECs) (cell line), during H 2 O 2 -induced oxidative stress (Hafizah et al, 2010). Ten percent aqueous extract of the plant in a dose of 150 µg/mL has shown marked induction of NO in HUVECs (Ugusman et al, 2010).…”