Pinus residue/pectin-based composite hydrogels for the immobilization of β-D-galactosidase

Cargnin, Mariana Aguiar; Souza, Alana G.; Lima, Giovanni F. de; Gasparin, Bruna Carla; Rosa, Derval dos Santos; Paulino, Alexandre Tadeu

doi:10.1016/j.ijbiomac.2020.01.280

Cited by 27 publications

(11 citation statements)

References 57 publications

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“…β S e (11) in which q ms (mg g −1 ) is the maximum Sips adsorption capacity, K s (L mg −1 ) is the Sips equilibrium constant, and β s is the Sips exponent employed to explain the homogeneity/heterogeneity of the adsorption system [29].…”

Section: Sips Isothermmentioning

confidence: 99%

“…Hydrogel porous chemical structures might be responsive to, for instance, ionic strength, pH and temperature, by swelling and thus facilitating the release processes of encapsulated drugs [9,10]. Natural polysaccharides are potential matrices for hydrogel synthesis as they are biocompatible, biodegradable, non-toxic, eco-friendly and inexpensive compounds [11]. Chitosan is one of the most applied polysaccharides in hydrogel synthesis due to its antimicrobial activity, biodegradability and biocompatibility for living beings [12].…”

Section: Introductionmentioning

confidence: 99%

“…Pectin is a widely produced natural polysaccharide from primary cell walls of citrus peels, which are considered agro-industrial residues. Hydrogels can be prepared by the coacervation technique using mixtures of polysaccharides such as pectin and chitosan [11] and mixtures of polysaccharides and deoxyribonucleic acid (DNA). DNA is a biopolymer containing deoxyribose sugar capable of carrying genetic information, which is useful in hydrogel synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Methylene Blue Release from Chitosan/Pectin and Chitosan/DNA Blend Hydrogels

2021

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Chitosan/DNA blend hydrogel (CDB) and chitosan/pectin blend hydrogel (CPB) were synthesized using an emulsion (oil-in-water) technique for the release of methylene blue (model molecule). Both hydrogels were characterized by swelling assays, Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM), before and after the methylene blue (MB) loading. Higher swelling degrees were determined for both hydrogels in simulated gastric fluid. FT-IR spectra inferred absorption peak changes and shifts after MB loading. The TGA results confirmed changes in the polymer network degradation. The SEM images indicated low porosities on the hydrogel surfaces, with deformed structure of the CPB. Smoother and more uniform surfaces were noticed on the CDB chain after MB loading. Higher MB adsorption capacities were determined at lower initial hydrogel masses and higher initial dye concentrations. The MB adsorption mechanisms on the hydrogel networks were described by the monolayer and multilayer formation. The MB release from hydrogels was studied in simulated gastric and intestinal fluids, at 25 °C and 37 °C, with each process taking place at roughly 6 h. Higher release rates were determined in simulated gastric fluid at 25 °C. The release kinetics of MB in chitosan/DNA and chitosan/pectin matrices follows a pseudo-second-order kinetic mechanism.

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Section: Sips Isothermmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Methylene Blue Release from Chitosan/Pectin and Chitosan/DNA Blend Hydrogels

2021

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“…Such hydrogels have also been used to immobilize urease [ 26 , 27 , 28 ]. Cargnin et al [ 29 ] explored the potential of pinus residue/pectin-based bio-composite hydrogel matrices to immobilize β- d -galactosidase. The developed hydrogels comprising 0%, 5% and 10% of pinus residue showed enzyme immobilization abilities of 242.08, 181.27 and 182.71 mg enzyme/g dried hydrogel.…”

Section: Hydrogels For Biocatalysismentioning

confidence: 99%

Expanding the Biocatalytic Scope of Enzyme-Loaded Polymeric Hydrogels

Tan

Bilal

Raza

et al. 2021

Gels

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In recent years, polymeric hydrogels have appeared promising matrices for enzyme immobilization to design, signify and expand bio-catalysis engineering. Therefore, the development and deployment of polymeric supports in the form of hydrogels and other robust geometries are continuously growing to green the twenty-first-century bio-catalysis. Furthermore, adequately fabricated polymeric hydrogel materials offer numerous advantages that shield pristine enzymes from denaturation under harsh reaction environments. For instance, cross-linking modulation of hydrogels, distinct rheological behavior, tunable surface entities along with elasticity and mesh size, larger surface-volume area, and hydrogels’ mechanical cushioning attributes are of supreme interest makes them the ideal candidate for enzyme immobilization. Furthermore, suitable coordination of polymeric hydrogels with requisite enzyme fraction enables pronounced loading, elevated biocatalytic activity, and exceptional stability. Additionally, the unique catalytic harmony of enzyme-loaded polymeric hydrogels offers numerous applications, such as hydrogels as immobilization matrix, bio-catalysis, sensing, detection and monitoring, tissue engineering, wound healing, and drug delivery applications. In this review, we spotlight the applied perspective of enzyme-loaded polymeric hydrogels with recent and relevant examples. The work also signifies the combined use of multienzyme systems and the future directions that should be attempted in this field.

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“…Immobilization of enzymes is an easy procedure with several benefits, including enzyme reusability, persistent process, increased stability under operation and storage state. 15 - 18 …”

Section: Enzymesmentioning

confidence: 99%

Recent Advances of Macromolecular Hydrogels for Enzyme Immobilization in the Food Products

et al. 2021

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Enzymes are one of the main biocatalysts with various applications in the food industry. Stabilization of enzymes on insoluble carriers is important due to the low reuse, low operational stability, and high cost in applications. The immobility and the type of carrier affect the activity of the immobile enzyme. Hydrogels are three-dimensionally cross-linked macromolecular network structures designed from various polymers. Hydrogels can provide a matrix for an immobile enzyme due to their extraordinary properties such as high water absorbing capacity, carrier of bioactive substances and enzymes, biocompatibility, safety, and biodegradability. Therefore, this study mainly focuses on some enzymes (Lactase, Lipases, Amylases, Pectinase, Protease, Glucose oxidase) that are of special importance in the food industry. These enzymes could be immobilized in the hydrogels constructed of macromolecules such as kappa-carrageenan, chitosan, arabic gum, pectin, alginate, and cellulose. At last, in the preparation of these hydrogels, different enzyme immobilization methods in macromolecular hydrogels, and effect of hydrogels on enzyme activity were discussed.

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Pinus residue/pectin-based composite hydrogels for the immobilization of β-D-galactosidase

Cited by 27 publications

References 57 publications

Methylene Blue Release from Chitosan/Pectin and Chitosan/DNA Blend Hydrogels

Methylene Blue Release from Chitosan/Pectin and Chitosan/DNA Blend Hydrogels

Expanding the Biocatalytic Scope of Enzyme-Loaded Polymeric Hydrogels

Recent Advances of Macromolecular Hydrogels for Enzyme Immobilization in the Food Products

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