Pinus massoniana bark extract inhibits migration of the lung cancer A549 cell line

Mao, Ping; Zhang, Ershao; Chen, Yang; Liu, Likun; Da-qing, Rong; Li, Qingfeng; Li, Weiling

doi:10.3892/ol.2016.5509

Cited by 12 publications

(7 citation statements)

References 28 publications

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“…For that three different cell lines, one normal mouse cell line (L929) and two human cell lines (normal-HEK293T-and derived from cancer tissue-A549) were used. Several authors have used similar cell lines to evaluate the potential toxicity of other plant extracts and isolated products [56,57]. Different concentrations of active extracts were placed in contact with the cells and their ability to metabolize resazurin into resorufin in the presence of PBE was used as a measurement of cells metabolic activity.…”

Section: Cell Viabilitymentioning

confidence: 99%

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Unravelling the Biological Potential of Pinus pinaster Bark Extracts

et al. 2020

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Natural compounds from agro-food by-products have fostered interest in food industries. The aim of this study was to unravel potential uses for Pinus pinaster bark extracts (PBE). As functional features of this type of extracts are usually attributed to phenolic compounds, the extraction process was studied. Different PBEs were achieved, with high content in phenolic compounds, using different water/ethanol combinations as a solvent. These PBEs were chemically characterized, and their bioactivity and in vitro cell viability were evaluated. Extracts obtained with hydroethanolic solvents had higher content in phenolic and flavonoid compounds. All the PBEs presented high antioxidant, antibacterial and antihyperglycemic activities. Moreover, PBEs have low cytotoxicity and a selective activity against cancer cells as these were negatively affected. These features may allow the extracts to be used in food formulation and processing (as preservatives, antioxidants or bioactive ingredients), but they showed also potential for the pharmaceutical or nutraceutical sectors.

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Section: Cell Viabilitymentioning

confidence: 99%

“…Touriño and collaborators [60] reported that P. pinaster bark extracts have a high antioxidant activity and can control proliferation in a human melanoma cell line. Additionally, Mao et al [57] demonstrated that extracts from the Pinus massoniana bark inhibit migration of the lung cancer A549 cell line.…”

Section: Cell Viabilitymentioning

confidence: 99%

Unravelling the Biological Potential of Pinus pinaster Bark Extracts

et al. 2020

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“…The samples were analyzed using a FACScan flow cytometer. On the basis of DNA content, the percentage of cells in each stage in the cell cycle (G 1 , S, and G 2 /M) was calculated using ModFit Lt. 3.0 s [ 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

The anticancer activities of Vernonia amygdalina Delile. Leaves on 4T1 breast cancer cells through phosphoinositide 3-kinase (PI3K) pathway

Hasibuan

Harahap

Sitorus

et al. 2020

Heliyon

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Vernonia amygdalina Delile ( Asteraceae ) is used in traditional medicine to treat diabetes mellitus, and some research provides its activity to treat breast cancer. The aim of this study is to assess the anticancer activity of Vernonia amygdalina Delile leaves fractions on 4T1 breast cancer cells. Analysis of phytochemical compounds were carried out with LC-MS/MS. Cytotoxic activity was determined using the MTT method in the 4T1 cell line. Apoptosis, the cell cycle, and PI3K and mTOR profiles were analyzed with flow cytometry. The phytochemicals found were diterpene (ingenol-3-angelate) and some phenolics (chlorogenic acid and 4-methoxycinnamic acid), flavonoids (apigetrin, apigenin, luteolin, diosmetin, baicalin, rhoifolin, and scutellarin), and coumarines (7-hydroxycoumarine, 4-methylumbelliferone, and 4-methylumbelliferyl glucuronide). The results of the MTT assay showed that the IC 50 values n -hexane fraction, ethylacetate fraction (EAF), and ethanol fractions were 1,860.54 ± 93.11, 25.04 ± 0.36, and 1,940.84 ± 96.37 μg/mL, respectively. EAF induced early and late apoptosis, inhibited cell cycle progression on the G 2 /M phase, and inhibited PI3K and mTOR expression. The EAF of Vernonia amygdalina Delile leaves showed anticancer activity on 4T1 breast cancer cells through induction of apoptosis, enhanced cell accumulation on G 2 /M phases in the cell cycle, and inhibited expression of PI3K and mTOR.

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“…PMBE was demonstrated to exhibit various bioactivities such as antiviral, anti-allergic, antioxidant and anticancer [2,7,8] . It has been proven that PMBE mainly induces cancer cells cycle arrest at S and G2/M phase [9] , further induces apoptosis of cancer cells via mitochondrial pathway and / or death receptor pathway [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Effects of Pinus massoniana bark extract on the size of HeLa cells via Nesprin-2 pathway

Zhang

et al. 2020

Curr Cancer Rep

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Since the 2nd half of the 16th century, over more than 300 hundred years of exploration and accumulation, research has entered the stage of revealing the nature of objects from observing the surface phenomena. Meanwhile, hypothesis-driven research (HDR) has gradually become the main form of research in many fields of modern science. HDR begins with familiarizing the background knowledge, according to the direction of the problem, using rational thinking methods to generalize the known relevant scientific phenomena and laws and build scientific hypotheses, then carrying out theoretical deduction and prediction, and then designing corresponding research programs including scientific observation, scientific experiment or scientific investigation, so as to test hypotheses and further obtain the answers. Usually, the formation of scientific hypothesis includes two stages: the initial hypothesis and the complete hypothesis. The former is only based on a small number of factual materials and existing relevant theories, and puts forward a tentative hypothesis for the answer of questions through thinking processing, which has not yet constituted a systematic discussion. Further, the initial hypothesis mainly includes the following four basic links: accumulating facts, mastering knowledge; analyzing facts, sorting out data; guessing, drawing conclusions; constructing concepts, expressing hypotheses. After putting forward the initial hypothesis, researchers should take this as the center, use various existing scientific theories and as many relevant background knowledge or conditions as possible to carry out extensive argumentation, explain the known facts, predict the unknown facts and potential rules or laws, and strive to theorize and systematize them, so as to expand the initial hypothesis into a relatively complete and stable theoretical system. In brief, the formation of scientific hypothesis is a process of describing facts, explaining facts, and finding causal links and objective laws. Therefore, it requires objective analysis of problems, comprehensive collection of materials and in-depth processing, and on this basis, a comprehensive grasp of the links between things. Considering the fact that the ability to propose hypotheses is an important feature of innovative talents, budding researchers should be trained from endowing them with capabilities for putting forward hypothesis. Just in this sense, CCR plans to select some hypothesis papers for publication to encourage more graduates or even undergraduates to express their special observation perspectives and innovative thinking on carcinogenesis, cancer prevention and treatment, further help them develop into mature researchers as soon as possible. The subsequent hypothesis is a good case in point.

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Pinus massoniana bark extract inhibits migration of the lung cancer A549 cell line

Cited by 12 publications

References 28 publications

Unravelling the Biological Potential of Pinus pinaster Bark Extracts

Unravelling the Biological Potential of Pinus pinaster Bark Extracts

The anticancer activities of Vernonia amygdalina Delile. Leaves on 4T1 breast cancer cells through phosphoinositide 3-kinase (PI3K) pathway

Effects of Pinus massoniana bark extract on the size of HeLa cells via Nesprin-2 pathway

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