Pinisolibacter ravus gen. nov., sp. nov., isolated from pine forest soil and allocation of the genera Ancalomicrobium and Pinisolibacter to the family Ancalomicrobiaceae fam. nov., and emendation of the genus Ancalomicrobium Staley 1968

Abstract

A novel bacterium, designated strain E9, was isolated from pine forest soil of Kyonggi University (Suwon, Republic of Korea). Cells were facultatively anaerobic, Gram-staining-negative, catalase-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and straw coloured. Prosthecae were absent. Glucose was fermented. The strain grew in the pH range of 5.0-10.0 (optimum, 6.5-8.5) and at 45 °C (optimum, 28-32 °C). E9 was sensitive to NaCl at low concentration and tolerated only 0.2 % NaCl (w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that E9 formed a lineage within the phylum Proteobacteria that was distinct from various members of the order Rhizobiales, including Ancalomicrobium adetum DSM 4722 (94.76 % sequence similarity), 'Nitratireductor lucknowense' IITR-21 (92.72 %), Prosthecomicrobium hirschii 16 (92.66 %) and Kaistia soli DSM 19436 (92.53 %). The predominant isoprenoid quinone was Q-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl-N-methylethanolamine. Major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), and C16 : 0. The DNA G+C content of the type strain was 68.4 mol%. Polyphasic characterization indicated that strain E9 represented a novel species in a novel genus within a novel family, for which the name Pinisolibacter ravus gen. nov., sp. nov. is proposed. The type strain of Pinisolibacter ravus is E9 (=KEMB 9005-534=KACC 19120=NBRC 112686). A formal allocation of the genus Ancalomicrobium to the family Ancalomicrobiaceae fam. nov. is also proposed.

Chitinophaga fulva sp. nov., isolated from forest soil

“…After 4 weeks, the enriched culture was serially diluted and then 100 µl of each dilution was spread on 1/10 R2A agar and incubated at 28 °C for 4 weeks [8]. The short-term maintenance and long-term preservation were done as described previously [9].…”

Nine novel psychrotolerant species of the genus Pedobacter isolated from Arctic soil with potential antioxidant activities

“…The type strains were deposited in various culture collections and can be obtained for study. Isolation and preservation of strains were done as previously described [11].…”

Characterization of Terrihabitans soli gen. nov., sp. nov., a Novel 0.2 μm-Filterable Soil Bacterium Belonging to a Widely Distributed Lineage of Hyphomicrobiales (Rhizobiales)

“…In the API ZYM, both strains were positive for alkaline phosphatase, esterase, esterase lipase, acid phosphatase, and naphthol-AS-BIphosphohydrolase, and were negative for all tests except for the enzymatic activities, where differences were observed as shown below. Data from two other strains of the Hyphomicrobiales (Rhizobiales) were used for comparison purposes; 3, Pinisolibacter [36]; 4, Methylopila [37].…”