Nine novel psychrotolerant species of the genus Pedobacter isolated from Arctic soil with potential antioxidant activities

Dahal, Ram Hari; Chaudhary, Dhiraj Kumar; Kim, Donguk; Kim, Jaisoo

doi:10.1099/ijsem.0.004071

Cited by 42 publications

(12 citation statements)

References 53 publications

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“…Interestingly, differences were found between the fatty acid profiles of P. frigidisoli CCM 9055 T obtained in this study and the original description of this species. The main differences are connected to the fatty acid C 18 : 1 ω7c 11-methyl, which was not reported in the original description for the type strain of P. frigidisoli [4], and summed feature 8 (C 18 : 1 ω7c/C 18 : 1 ω6c), which was only found in a trace amount (<1 %), while it was 10.8 % in this analysis. These differences might be related to the different cultivation temperature used for the cultivation of P. frigidisoli RP-3-11 T in the original description compared to this analysis, which is known to significantly affect the cell membrane fatty acids composition, as does media composition [56,57].…”

Section: Physiology and Chemotaxonomycontrasting

confidence: 73%

“…The solvent system used for the first dimension of TLC was chloroform-methanol-water (65 : 25 : 4, v/v/v) and for the second dimension of TLC chloroform-acetic acid-methanol-water (80 : 15 : 12 : 4, v/v/v/v). Total lipids were detected by spraying the TLC plate with molybdatophosphoric acid reagent, phospholipids *The opposite results were reported for P. frigidisoli [4] and P. terrae [61].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 94%

“…Similarly, the polar lipid profile of the phylogenetically closest P. frigidisoli RP-3-11 T [4] contained phosphatidylethanolamine as the major lipid and the presence of aminolipids, aminoglycolipids, and glycolipids was observed, as with strain P8930 T . However, both strains did not exhibit the presence of sphingolipids.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…The number of new Pedobacter species with validly published names has increased considerably in recent years [3], with many of them isolated from polar habitats covering both the Arctic and Antarctic regions. For example, of the 12 species names validated from January to September 2020, nine species are from the Arctic [4] and two from the Antarctic [5, 6] environment.…”

Section: Full-textmentioning

confidence: 99%

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Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

Švec

Králová

Staňková

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA–DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).

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Section: Physiology and Chemotaxonomycontrasting

confidence: 73%

Section: Physiology and Chemotaxonomymentioning

confidence: 94%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Full-textmentioning

confidence: 99%

See 2 more Smart Citations

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

Švec

Králová

Staňková

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…At the time of writing, this genus has been amended eight times and comprises 87 validly published species (https://lpsn.dsmz.de/genus/pedobacter). Members of the genus Pedobacter are widely distributed in various environments, such as soil, drinking water, deep-sea sediment and plant roots [2–7]. Some strains of the genus Pedobacter can even exist under extreme environmental conditions, such as alpine glacier cryoconite [8].…”

Section: Full-textmentioning

confidence: 99%

Pedobacter endophyticus sp. nov., an endophytic bacterium isolated from Carex pumila

Peng

Jiang

Seo

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, Gram-stain-negative, weak-motile, short-rod-shaped bacterial strain, designated JBR3-12T, was isolated from halophyte Carex pumila plants, and its taxonomic position was investigated by using a polyphasic taxonomic approach. The strain produced a pink pigment on tryptic soy agar and grew optimally at 25 °C, pH 8 and in the presence of 3 % (w/v) NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain JBR3-12T formed a lineage within the genus Pedobacter and was most closely related to Pedobacter sandarakinus DS-27T (98.0 %) and Pedobacter agri PB92T (97.6 %). The DNA G+C content of the genome was 41.3 mol%; the whole genome length was 5 426 070 bp. The major fatty acids of JBR3-12T were iso-C15 : 0, summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c) and iso-C17 : 0 3-OH. The predominant polar lipid was phosphatidylethanolamine. The predominant quinone was menaquinone-7. Based on its phenotypic, phylogenetic and genotypic features, strain JBR3-12T is proposed to represent a novel species of the genus Pedobacter , for which the name is Pedobacter endophyticus sp. nov. The type strain is JBR3-12T (=KCTC 82363T=NBRC 114901T).

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Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties

Choi

Kim

Song

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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Psychrophilic bacteria, living at low and mild temperatures, can contribute significantly to our understanding of microbial responses to temperature, markedly occurring in the bacterial membrane. Here, a newly isolated strain, Pseudomonas sp. B14-6, was found to dynamically change its unsaturated fatty acid and cyclic fatty acid content depending on temperature which was revealed by phospholipid fatty acid (PLFA) analysis. Genome sequencing yielded the sequences of the genes Δ-9-fatty acid desaturase (desA) and cyclopropane-fatty acid-acyl-phospholipid synthase (cfa). Overexpression of desA in Escherichia coli led to an increase in the levels of unsaturated fatty acids, resulting in decreased membrane hydrophobicity and increased fluidity. Cfa proteins from different species were all found to promote bacterial growth, despite their sequence diversity. In conclusion, PLFA analysis and genome sequencing unraveled the temperature-related behavior of Pseudomonas sp. B14-6 and the functions of two membrane-related enzymes. Our results shed new light on temperature-dependent microbial behaviors and might allow to predict the consequences of global warming on microbial communities.

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Nine novel psychrotolerant species of the genus Pedobacter isolated from Arctic soil with potential antioxidant activities

Abstract: Seven supplementary figures and three supplementary tables are available with the online verson of this article.

Cited by 42 publications

References 53 publications

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

Pedobacter endophyticus sp. nov., an endophytic bacterium isolated from Carex pumila

Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties

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