Pine Wilt Disease: A Threat to Pine Forests in Turkey?

Akbulut, Süleyman; Yüksel, Beşir; Baysal, İsmail; Vieira, Paulo; Mota, Manuel

doi:10.1007/978-1-4020-8455-3_4

Cited by 8 publications

(2 citation statements)

References 11 publications

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“…Pine wilt disease (PWD) is caused by pinewood nematode (PWN, Bursaphelenchus xylophilus ) and is a highly dangerous and devastating epidemic disease in forests ( Mota and Vieira, 2008 ; Jeong et al, 2013 ). PWNs invade, feed, and multiply rapidly in pine trees.…”

Section: Introductionmentioning

confidence: 99%

Comparative effects of two in situ hybridization methods for the pinewood nematode (Bursaphelenchus xylophilus)

Wang,

Zhuge,

Tang

et al. 2023

Front. Microbiol.

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The gene localization technique of Bursaphelenchus xylophilus (pinewood nematode, PWN) is used for study gene expression in PWNs. Two in situ hybridization methods, namely, whole-mount in situ hybridization and the cut-off method are used widely. To compare the effects of these two in situ hybridization methods, the present study investigated the patterns of two functional genes expression in PWNs. The Bx-vap-2 gene (GenBank accession number: OR228482), related to pathogenicity, and the fem-2 gene (GenBank accession number: OR228481), related to sex determination, were selected to map related genes in the whole-mount and amputated PWNs at different ages using these in situ hybridization methods. Based on the overall statistical comparison, we found that compared to the cut-off method, the whole-mount method exhibited higher staining rates and correct staining rates for the fem-2 gene and Bx-vap-2 gene. However, considering the correct staining aspect, the cut-off method yielded better staining effects on pinewood nematode sections than the whole-mount method, with clearer hybridization signal locations and less non-specific staining. In other words, the cut-off method demonstrated more precise gene localization. Both methods are applicable for gene localization, but considering the overall staining pattern, analysis of experimental results, and comprehensive experimental operations, we believe that the whole-mount method is more suitable for gene localization and expression analysis of development-related genes in pinewood nematodes. This is because intact pinewood nematodes are better suited for showcasing the continuous developmental process of development-related genes. On the other hand, considering the experimental time, accuracy of staining site, and the amount of non-specific staining, the cut-off method is more suitable for disease-related genes. Additionally, to achieve better performance, the cut-off method can be selectively applied to samples during the experimental process.

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Section: Introductionmentioning

confidence: 99%

Comparative effects of two in situ hybridization methods for the pinewood nematode (Bursaphelenchus xylophilus)

Wang,

Zhuge,

Tang

et al. 2023

Front. Microbiol.

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“…For instance, the genus Monochamus (Cerambycidae) is best known for its fundamental role in the epidemiology of pine wilt disease ( Mamiya, 1983 ). Specific species of the genus are responsible for the dissemination of the pinewood nematode, Bursaphelenchus xylophilus ( Togashi, 2008 ). In Korea, for instance, M. alternatus and M. saltuarius are the main vectors of the pine wood nematode, and the former is mainly distributed in the southern region and the latter in the central region ( Kwon et al, 2006 ).…”

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confidence: 99%

Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea

Mwamula¹,

Lee²,

Jung³

et al. 2023

Journal of Nematology

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Diplogasteroides sp., a cryptic population of D. haslacheri, and Parasitorhabditis terebranus were reported from the frass of Monochamus alternatus galleries in dead Pinus thunbergii for the first time in Korea. Females and males are morphologically characterized and their linked DNA barcodes (18S-rRNA, 28S-rRNA, ITS-rRNA and COI) supplied. Females and males of the two species from Korea conform to the original species descriptions from Europe and the USA, with variations in a few details in morphometrics. Specifically, Diplogasteroides sp. is morphologically very similar to D. haslacheri. However, it cannot be designated as D. haslacheri due to the existence of cryptic species complex within the haslacheri group (D. haslacheri, D. asiaticus, D. nix, D. andrassyi, and D. carinthiacus), a condition requiring hybridization studies to test species identity within the group. Based on analysis of COI sequences, differences among these cryptic species are evident. Thus, in addition to hybridization tests, the COI might be a powerful DNA barcoding marker for the precise identification of these cryptic species within the genus. Additionally, this is the first molecular characterization of P. terebranus, and the species is herein recorded for the first time outside its type locality.

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Deep learning combined with Balance Mixup for the detection of pine wilt disease using multispectral imagery

Rao

Zhang

et al. 2023

Computers and Electronics in Agriculture

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Pine Wilt Disease: A Threat to Pine Forests in Turkey?

Cited by 8 publications

References 11 publications

Comparative effects of two in situ hybridization methods for the pinewood nematode (Bursaphelenchus xylophilus)

Comparative effects of two in situ hybridization methods for the pinewood nematode (Bursaphelenchus xylophilus)

Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea

Deep learning combined with Balance Mixup for the detection of pine wilt disease using multispectral imagery

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