Comparative effects of two in situ hybridization methods for the pinewood nematode (Bursaphelenchus xylophilus)
Chunyu Wang,
Junhao Zhuge,
Siqi Tang
et al.
Abstract:The gene localization technique of Bursaphelenchus xylophilus (pinewood nematode, PWN) is used for study gene expression in PWNs. Two in situ hybridization methods, namely, whole-mount in situ hybridization and the cut-off method are used widely. To compare the effects of these two in situ hybridization methods, the present study investigated the patterns of two functional genes expression in PWNs. The Bx-vap-2 gene (GenBank accession number: OR228482), related to pathogenicity, and the fem-2 gene (GenBank acc… Show more
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