Pilot study of long-term anaesthesia in broiler chickens

O'Kane, Peter M; Connerton, Ian F.; White, Kate

doi:10.1111/vaa.12305

Cited by 8 publications

(3 citation statements)

References 7 publications

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“…The supernatants were collected, filtered through a 0.22-μm-pore-size syringe filter (Millipore, USA) and inoculated into 7-day-old specific pathogen-free (SPF) chicken embryos which were collected from the SPF Experimental Animal Center (Wens Dahuanong Biotechnology Co., Ltd., Guangdong, China) as previously described [18]. All animals used in this study were unconscious and anaesthesia was maintained with isoflurane in oxygen, and then euthanized by parenteral pentobarbitone at the end of experiment as previously described [20]. Animal experiments were approved by local institutional ethical committee to conduct experiments in chicken embryo and poultry birds as per ethical requirement.…”

Section: Methodsmentioning

confidence: 99%

Epidemiological investigation of fowl adenovirus infections in poultry in China during 2015–2018

et al. 2019

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Background Fowl adenoviruses (FAdVs) are associated with many diseases, resulting in huge economic losses to the poultry industry worldwide. Since 2015, outbreaks of FAdV infections with high mortality rates have been reported in China. A continued surveillance of FAdVs contributes to understand the epidemiology of the viruses. Results We isolated 155 FAdV strains from diseased chickens from poultry in China between 2015 and 2018. PCR analysis determined that 123 samples were FAdV species C, 27 were FAdV species E, and five contained two different FAdV strains. The phylogenetic analysis demonstrates that these sequences of hexon regions were clustered into three distinct serotypes: FAdV-4 (79.4%, 123/155), FAdV-8a (13.5%, 21/155) and FAdV-8b (3.9%, 6/155), of which FAdV-4 was the dominant serotype in China. Conclusions The characterization of newly prevalent FAdV strains provides valuable information for the development of an effective control strategy for FAdV infections in chickens. Electronic supplementary material The online version of this article (10.1186/s12917-019-1969-7) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Epidemiological investigation of fowl adenovirus infections in poultry in China during 2015–2018

et al. 2019

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“…Found in medicinal plants, including anemones, it stimulates beta cells in the liver to increase insulin secretion to reduce glucose formed during stress [29], [30]. The lungs to the cells of the body and carbon dioxide from the cells of the body to the lungs, thus maintaining the ratio of PCV, Hb and hemoglobin is important in transporting and supplying oxygen for cellular respiration [31], and this is noted in Table (1,2) and it is noted that the percentage of total protein, albumin and globulin is maintained higher than the two control treatments. These results agree with [32], [33] where they indicated that medicinal plants preserve proteins.…”

Section: Discussionmentioning

confidence: 99%

Spraying with local anemone coronaria flower extract and isoflurane and its effect on the physiological characteristics of broiler chickens transported during different seasons

Tamimi¹,

Shanoun²,

Mohammed³

2023

kujas

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The effect of the extract Anemone coronaria and Isoflurane on the transport stress of broiler chickens transported during different seasons of the year was tested, The field experiment was conducted throughout the different seasons of the year during the months of February, March, July, August and with different densities in the transport cage. where the birds of type 308 ROSS were divided by 10 treatments for each transfer and three replicates for each treatment. And as follows, T1 control without spraying and 10 birds per cage. T2 control without spraying and the number of birds 12 in the cage. T3 Spraying the birds with an extract of local Anemone coronaria flowers at a concentration of 2% and the number of birds is 10 in the cage. T4 Spraying the birds with an extract of local Anemone coronaria flowers at a concentration of 4% and the number of birds 10 in the cage. T5 Spraying the birds with an extract of local Anemone coronaria flowers at a concentration of 6% and the number of birds 10 in the cage. T6 Spraying the birds with an extract of local Anemone coronaria flowers at a concentration of 2%. The number of birds is 12 in the cage. T7 Spraying the birds with an extract of local Anemone coronaria flowers at a concentration of 4%. The number of birds is 12 in the cage. T8 Spraying the birds with an extract of local Anemone coronaria flowers at a concentration of 6% and the number of birds is 12 in the cage. T9 Spraying birds with isoflurane at a concentration of 6%, the number of birds is 10 per cage. T10 Spraying birds with isoflurane at a concentration of 6%. The number of birds is 12 per cage ,The birds were sprayed for 5 minutes before the transfer process, according to the above treatments, and the duration of one transfer was 240 minutes ± 10 minutes,while these transactions led to a highly significant decrease (p>0.01) in the general rate of heterophile, as it recorded (34.79, 35.20 , 36.03%), respectively, compared with the two control treatments (38.62, 38.87) and in the heterophile/lymphocide ratio, as T9, T10, T5 recorded (0.66, 0.67, 0.69) compared with T1, T2 (0.78, 0.79), as well as a highly significant decrease in glucose (p>0.01), as T9, T10, and T5 were recorded (198.82, 200.64, 205.83) compared to T1, T2 (226.71, 229.24). A highly significant (p>0.01) superiority was observed in lymphocytes, as T9, T10, and T5 were recorded. (52.79, 52.56, 52.24) compared to T1 and T2 (49.08, 48.64)%, respectively. As for hemoglobin, T9, T10, T5 (9.42, 9.39, 9.02) gm/100 ml were superior to T1, T2 (8.99, 8.96) gm/100 ml. As well as a significant superiority in the percentage of packed volume cells, as it recorded T9, T10, T5 (34.15, 34.04, 33.06) compared with T1, T2 (30.96, 29.59)%, respectively.Also, there is a crease in the total protein concentration (2.71, 2.62, 2.50, ) g/dl compared with T1 and T2 (2.06, 2.00) g/dl, respectively. It is noted from this experiment that the treatments of isoflurane and Anemone coronaria extract led to a reduction in the stress that affects birds during transport operations, and thus was reflected in the improvement of the productive performance and health of the transported birds.

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“…On day 15, 3 broilers from each cage were randomly selected (9 broilers from 3 cages for each treatment) for the collection of samples to evaluate the effect of gEGF on their immune function and intestinal development. Blood was collected from the wing vein of all randomly selected broilers before they were anaesthetized according to O’Kane’s method [ 27 ] and sacrificed by exsanguinations for the sample collection of intestinal tissues and immune organs. Care and maintenance of all animals were in line with the Guide for the Care and Use of Laboratory Animals Monitoring Committee of Hubei Province, China.…”

Section: Methodsmentioning

confidence: 99%

Expression of Gallus Epidermal Growth Factor (gEGF) with Food-Grade Lactococcus lactis Expression System and Its Biological Effects on Broiler Chickens

Chen

Shi

et al. 2021

Biomolecules

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As a multifunctional polypeptide, epidermal growth factor (EGF) increases growth performance or enhances resistance to diseases in commercial broilers under adverse conditions. In this study, a recombinant Lactococcus lactis was established to produce the secretory form of bioactive gEGF. The results of in vitro testing showed that gEGF promoted the proliferation of chicken embryo fibroblast cells. A total of 63 5-day-old broiler chickens were evenly divided into three groups and treated with either M17 medium (the control group), supernatant of LL-pNZ8149 fermentation product (the P-LL group), or supernatant of LL-pNZ8149-gEGF fermentation product (the gEGF group). In two weeks, many measurements of growth, immunity and the intestines were significantly higher in the gEGF group than those in the control and the P-LL groups. Our study showed that the bioactive gEGF could be expressed with Lactococcus lactis expression system with the potential to enhance growth performance, immune function, and intestinal development in broiler chickens.

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Pilot study of long-term anaesthesia in broiler chickens

Cited by 8 publications

References 7 publications

Epidemiological investigation of fowl adenovirus infections in poultry in China during 2015–2018

Epidemiological investigation of fowl adenovirus infections in poultry in China during 2015–2018

Spraying with local anemone coronaria flower extract and isoflurane and its effect on the physiological characteristics of broiler chickens transported during different seasons

Expression of Gallus Epidermal Growth Factor (gEGF) with Food-Grade Lactococcus lactis Expression System and Its Biological Effects on Broiler Chickens

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