Expression of Gallus Epidermal Growth Factor (gEGF) with Food-Grade Lactococcus lactis Expression System and Its Biological Effects on Broiler Chickens

Abstract

As a multifunctional polypeptide, epidermal growth factor (EGF) increases growth performance or enhances resistance to diseases in commercial broilers under adverse conditions. In this study, a recombinant Lactococcus lactis was established to produce the secretory form of bioactive gEGF. The results of in vitro testing showed that gEGF promoted the proliferation of chicken embryo fibroblast cells. A total of 63 5-day-old broiler chickens were evenly divided into three groups and treated with either M17 medium (the control group), supernatant of LL-pNZ8149 fermentation product (the P-LL group), or supernatant of LL-pNZ8149-gEGF fermentation product (the gEGF group). In two weeks, many measurements of growth, immunity and the intestines were significantly higher in the gEGF group than those in the control and the P-LL groups. Our study showed that the bioactive gEGF could be expressed with Lactococcus lactis expression system with the potential to enhance growth performance, immune function, and intestinal development in broiler chickens.

Research Note: Preliminary functional analysis of EGF-like domains of Eimeria tenella microneme protein 7 identified in sporozoites and merozoites

“…As shown in Figure 2 D, the LMH cell proliferation was significantly enhanced by the treatment of rEGF-EtMIC7 with a concentration of 0.1 µg/mL. The results showed that EGF-EtMIC7 is similar to chicken EGF, because both can promote the proliferation of the chicken cell lines ( Zhou et al, 2021 ). In addition, Akt activation was reported to affect cell proliferation ( Wee and Wang, 2017 ).…”

“…The effects of rEGF-EtMIC7 on the proliferation of LMH cells were examined with Cell Counting Kit-8 ( CCK-8 ) assay ( Zhou et al, 2021 ). Briefly, LMH cells were cultured in DMEM with 10% FBS for 24 h at 37°C.…”

Better intestine health in fast‐growing bullfrogs ( Lithobates catesbeianus ) fed a soybean meal‐based diet than slow‐growing bullfrogs

“…Lactobacillus was proven to produce lactic acid, thereby decreasing the pH value of the intestinal tract, which was consistent with the results of the current study (Sha et al, 2016). There were several reports indicating that dietary supplementation of Lactobacillus improved the host gut health, feed utilization, growth, immune status and survival (Gajbhiye & Kapadnis, 2021; Lebeer et al, 2008; Parma et al, 2020; Zhou et al, 2021). Higher abundances of potentially pathogenic bacteria such as Pseudomonas , Escherichia‐Shigella , Mycoplasma , Acinetobacter , Vibrio and Sneathia were detected in the SG group.…”

Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression in Bacillus subtilis DB104 through Transcriptome Analysis

“…For example, Hu et al [44] reported the production of fibroblast EGF using an IPTG-inducible promoter in B. subtilis, with a yield of 31 mg/L. Although a nisin-inducible expression system in Lactococcus lactis could reach a productivity of 2.67 mg/L [45], it requires the addition of 10 ng/mL nisin to induce human EGF (hEGF) production.…”