Pilot study for three-dimensional assessment of laminar pore structure in patients with glaucoma, as measured with swept source optical coherence tomography

Omodaka, Kazuko; Maekawa, Shigeto; An, Guangzhou; Tsuda, S.; Shiga, Yukihiro; Takada, Naoko; Kikawa, Tsutomu; Takahashi, Hidetoshi; Yokota, Hideo; Nakazawa, Toru

doi:10.1371/journal.pone.0207600

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…A retrospective study showed that differences in LC pore shape and size of glaucomatous eyes have also been associated with damage to ON [ 12 ] using optic disc photographs. Based on images obtained by swept-source optical coherence tomography (OCT), Omodaka et al found that glaucoma patients had a smaller area and 3D volume of LC pores, probably due to the expanding area of connective tissue [ 13 ]. Elongation of lamina pores was more evident in primary open-angle glaucoma eyes than in normal eyes [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological Changes of Glial Lamina Cribrosa of Rats Suffering from Chronic High Intraocular Pressure

Zhang

Liu

et al. 2022

Bioengineering

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Deformations or remodeling of the lamina cribrosa (LC) induced by elevated intraocular pressure (IOP) are associated with optic nerve injury. The quantitative analysis of the morphology changes of the LC will provide the basis for the study of the pathogenesis of glaucoma. After the chronic high-IOP rat model was induced by cauterizing episcleral veins with 5-Fluorouracil subconjunctival injection, the optic nerve head (ONH) cross sections were immunohistochemically stained at 2 w, 4 w, 8 w, and 12 w. Then the sections were imaged by a confocal microscope, and six morphological parameters of the ONH were calculated after the images were processed using Matlab. The results showed that the morphology of the ONH changed with the duration of chronic high IOP. The glial LC pore area fraction, the ratio of glial LC pore area to the glial LC tissue area, first decreased at 2 w and 4 w and then increased to the same level as the control group at 8 w and continued to increase until 12 w. The number and density of nuclei increased significantly at 8 w in the glial LC region. The results might mean the fraction of glial LC beam increased and astrocytes proliferated at the early stage of high IOP. Combined with the images of the ONH, the results showed the glial LC was damaged with the duration of chronic elevated IOP.

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Section: Introductionmentioning

confidence: 99%

Morphological Changes of Glial Lamina Cribrosa of Rats Suffering from Chronic High Intraocular Pressure

Zhang

Liu

et al. 2022

Bioengineering

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OCT Technique: Past, Present and Future

Kostanyan¹,

Cadena²,

Wollstein³

et al. 2020

OCT and Imaging in Central Nervous System Diseases

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Lamina Cribrosa Microstructure in Nonhuman Primates With Naturally Occurring Peripapillary Retinal Nerve Fiber Layer Thinning

Alexopoulos,

Fernandes,

Ghassabi

et al. 2024

Trans. Vis. Sci. Tech.

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Purpose The lamina cribrosa (LC) is hypothesized to be the site of initial axonal damage in glaucoma with the circumpapillary retinal nerve fiber layer thickness (RNFL-T) widely used as a standard metric for quantifying the glaucomatous damage. The purpose of this study was to determine in vivo, 3-dimensional (3D) differences in the microstructure of the LC in eyes of nonhuman primates (NHPs) with naturally occurring glaucoma. Methods Spectral-domain optical coherence tomography (OCT) scans (Leica, Chicago, IL, USA) of the optic nerve head were acquired from a colony of 50 adult rhesus monkeys suspected of having high prevalence of glaucoma. The RNFL-T was analyzed globally and in quadrants using a semi-automated segmentation software. From a set of 100 eyes, 18 eyes with the thinnest global RNFL-T were selected as the study group and 18 eyes with RNFL-T values around the 50th percentile were used as controls. A previously described automated segmentation algorithm was used for LC microstructure analysis. Parameters included beam thickness, pore diameter and their ratio (beam-to-pore ratio [BPR]), pore area and shape parameters, beam and pore volume, and connective tissue volume fraction (CTVF; beam volume/total volume). The LC microstructure was analyzed globally and in the following volumetric sectors: quadrants, central and peripheral lamina, and three depth slabs (anterior, middle, and posterior). Results Although no significant difference was detected between groups for age, weight, or disc size, the study group had significantly thinner RNFL than the control group ( P < 0.01). The study group had significantly smaller global and sectoral pore diameter and larger BPR compared with the control group. Across eyes, the global RNFL-T was associated positively with pore diameter globally. BPR and CTVF were significantly and negatively associated with the corresponding RNFL-T in the superior quadrant. Conclusions Global and sectoral microstructural differences were detected when comparing thin and normal RNFL-T eyes. Whether these LC differences are the cause of RNFL damage or the result of remodeling of the LC requires further investigation. Translational Relevance Our findings indicate structural alterations in the LC of NHP exhibiting natural thinning of the RNFL, a common characteristic of glaucomatous damage.

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Pilot study for three-dimensional assessment of laminar pore structure in patients with glaucoma, as measured with swept source optical coherence tomography

Cited by 3 publications

References 31 publications

Morphological Changes of Glial Lamina Cribrosa of Rats Suffering from Chronic High Intraocular Pressure

Morphological Changes of Glial Lamina Cribrosa of Rats Suffering from Chronic High Intraocular Pressure

OCT Technique: Past, Present and Future

Lamina Cribrosa Microstructure in Nonhuman Primates With Naturally Occurring Peripapillary Retinal Nerve Fiber Layer Thinning

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