Pilot Plant Recovery of Soybean Oleosome Fractions by an Enzyme‐Assisted Aqueous Process

Towa, Lili T.; Kapchie, Virginie N.; Hauck, Catherine C.; Wang, Hui; Murphy, Patricia A.

doi:10.1007/s11746-010-1716-5

Cited by 21 publications

(24 citation statements)

References 32 publications

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“…The extraction was stopped when both fractions contained the minimal amount of oleosomes. The final aqueous supernatant and residue fractions were collected at the end of last extraction cycle as previously described by Towa and others (2011), then weighed and sampled for proximate and SDS‐PAGE protein analysis before being stored at −20 °C. This pilot‐plant oleosome fractionation process was replicated 3 times.…”

Section: Methodsmentioning

confidence: 99%

“…Towa and others (2011) described an intermediate and a proof‐of‐concept pilot scale process to fractionate kilogram quantities of full fat soybean flour. The pilot‐plant scale process used 75 kg of soybean flour and a three phase decanter centrifuge to recover oleosomes.…”

Section: Introductionmentioning

confidence: 99%

“…Oleosomes were continuously extracted for 6 h, and the centrifugation was stopped overnight. The next day, the residue and aqueous supernatant were mixed and centrifuged continuously for additional 4 h. These 2 d of extraction with manual mixed recirculation of the aqueous supernatant and residue resulted in a 93% semipurified oleosome extraction yield (Towa and others 2011). However, the process was long and labor intensive.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the distribution of phytochemicals in the oleosome isolation process has not been investigated. Therefore, the primary objective of the present study was to investigate the effectiveness and reproducibility of oleosome extraction using a modified pilot‐scale method based the one previously reported (Towa and others 2011). Further purification of oleosomes and identification of oleosins were examined.…”

Section: Introductionmentioning

confidence: 99%

“… Flow diagram of the pilot‐plant oleosome isolation procedure, A: procedure described by Towa and others (2011) ; B: improved fractionation process. …”

Section: Introductionmentioning

confidence: 99%

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Process Improvement for Semipurified Oleosomes on a Pilot‐Plant Scale

Kapchie¹,

Hauck²,

Wang³

et al. 2011

Journal of Food Science

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Semipurified oleosomes were isolated on a pilot-plant scale using improved-process extraction conditions. The improved process consisted of continuous centrifugation in a three-phase decanter with recirculation of slurry until most of the oleosomes were recovered. Oleosome fractionation, oleosin identification, and isoflavone and saponin mass distributions and recoveries were investigated. The improved pilot-plant oleosome extraction process was achieved in 8 h. A total of 91%± 1% of soybean oil was recovered as intact oleosomes. The oil content of the aqueous supernatant and the residue fractions were low at 2% and 3%, respectively. The aqueous supernatant fraction contained 40% total soybean protein. About 76% of the proteins present in the oleosome fraction were soybean storage proteins. Washing the semipurified oleosomes with a 0.1 M Tris-HCl, pH 8.6 containing 0.4 M sucrose, and 0.5 M NaCl resulted in the recovery of the associated storage proteins. The recovery of these proteins in addition to the protein in aqueous supernatant accounted for 79% of the total soybean storage proteins fractionated by this process. Oleosins were detected at 17 and 18 kDa. Isoflavones and saponins partitioned into the oleosome, aqueous supernatant, and residue fractions at different ratios with the majority, about 82 and 63 mole%, respectively, in oleosome and aqueous supernatant fractions, making these fractions an attractive source for phytochemicals.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“… Flow diagram of the pilot‐plant oleosome isolation procedure, A: procedure described by Towa and others (2011) ; B: improved fractionation process. …”

Section: Introductionmentioning

confidence: 99%

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Process Improvement for Semipurified Oleosomes on a Pilot‐Plant Scale

Kapchie¹,

Hauck²,

Wang³

et al. 2011

Journal of Food Science

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Recent developments and prospects in the extraction, composition, stability, food applications, and in vitro digestion of plant oil bodies

Hao

Wang

et al. 2022

J Americ Oil Chem Soc

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Oil bodies (OBs) are micron-or submicron-sized sub-organelles widely found in plants seeds and nuts. The structure of OBs is composed of a core of neutral lipids covered by a phospholipid-protein layer, which ensures the stability of OBs under extreme environmental conditions and further protects core lipids as energy reserves. As naturally pre-emulsified oil-in-water emulsions, OBs have been gradually applied to replace synthetically engineered oil droplets. In this paper, the recent research on the extraction, composition, stability, food applications, in vitro digestion, and future perspectives of plant OBs were reviewed. Recent studies have focused on the large-scale extraction techniques and OBs surface protein identification and function. Electrostatic deposition and viscosity effect of polysaccharides significantly improve the stability of OBs emulsions. OBs have broad application prospects in replacing milk fat droplets, as fat replacers and delivery vehicles, and fabricating emulsion-gels, oleogels or edible films. Notably, oleosins present a potential allergen risk. OBs are digested slowly, resulting in increased satiety and effectively helping to reduce calorie intake, but this can negatively affect the bioaccessibility of nutritional compounds in OBs. When OB as a delivery vehicle, its natural structure may be disrupted, whereas OBs-based delivery system facilitates the bioaccessibility of hydrophobic active compounds.

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Quantity and Quality of Free Oil Recovered from Enzymatically Disrupted Soybean Oleosomes

Towa¹,

Kapchie²,

Wang³

et al. 2011

J Americ Oil Chem Soc

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The operational variables impacting the quantity and quality of free oil recovered from isolated soybean oleosomes by enzymatic extraction were evaluated to optimize this process. Those variables were: protease concentration (0-2.5%), protease hydrolysis time (3 vs. 18 h), and slurry destabilization time (30 min vs. 3 h). Analysis of interactions between these variables and the yield of free oil revealed that the protease concentration was the most significant variable. The quantity of free oil extracted by using 3 h of oleosomes hydrolysis and 30 min of slurry destabilization was not significantly different from that using 18 h of oleosomes hydrolysis and 3 h of slurry destabilization. The optimum conditions, 0.5% Protex 6L, 3 h of hydrolysis, and 30 min of destabilization, resulted in 90% free oil. Oils extracted by the aqueous process had a fatty acid composition similar to conventional hexane-based process with oxidative stability indices ranging from 9 to 12 h. Enzyme assisted aqueous extraction resulted in a high quality oil which has 88% less free fatty acids than hexane-extracted oil. The optimal conditions generated 85.5% soybean storage proteins in skim with peptides smaller than 6.5 kDa and the degree of hydrolysis of 19.5%. The present study demonstrates that oil can be extracted from soybeans efficiently without hexane or other petroleum solvents.

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Pilot Plant Recovery of Soybean Oleosome Fractions by an Enzyme‐Assisted Aqueous Process

Cited by 21 publications

References 32 publications

Process Improvement for Semipurified Oleosomes on a Pilot‐Plant Scale

Process Improvement for Semipurified Oleosomes on a Pilot‐Plant Scale

Recent developments and prospects in the extraction, composition, stability, food applications, and in vitro digestion of plant oil bodies

Quantity and Quality of Free Oil Recovered from Enzymatically Disrupted Soybean Oleosomes

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