PIII-induced enhancement and inhibition of human cell attachment on chitosan membranes

Inthanon, Kewalin; Saranwong, N.; Wongkham, Weerah; Wanichapichart, Pikul; Prakrajang, K.; Suwannakachorn, D.; Yu, L.D.

doi:10.1016/j.surfcoat.2012.10.042

Cited by 12 publications

(7 citation statements)

References 24 publications

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“…This result was further verified by the increase of FAK expression described here (Figure 7), as filopodial shafts contain signaling pathways (e.g., FAK, Rac1/Cdc42/RhoA) promoting substrate adhesion [43]. Some studies have revealed that, in 3D environments, cells generally migrate into the substrate by producing various protrusion types, including some large adhesion patches called lamellipodia [41]. In fact, newly seeded cells transiently expressed filopodia for early attachment, which quickly disappeared [44] or converted into lamellipodia [40].…”

Section: Discussionsupporting

confidence: 81%

“…It is speculated that the surface of the 2D scaffolds provided anchorage points for initial attachment to hold the cell body in place, allowing subsequent filopodial protrusion to regulate cell shape and further growth [40]. Indeed, more numerous filopodial structures on 2D PCL scaffolds ( Figure 9) have been shown to promote greater cell attachment [41,42]. This result was further verified by the increase of FAK expression described here (Figure 7), as filopodial shafts contain signaling pathways (e.g., FAK, Rac1/Cdc42/RhoA) promoting substrate adhesion [43].…”

Section: Discussionmentioning

confidence: 99%

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Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Inthanon

Janvikul

Ongchai

et al. 2019

JFB

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Polycaprolactone (PCL), a hydrophobic-degradable polyester, has been widely investigated and extensively developed, to increase the biocompatibility for tissue engineering. This research was the first trial to evaluate the intrinsic biological responses of human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) cultured on alkaline hydrolysis and low-pressure oxygen plasma modified 2D and 3D PCL scaffolds, without adding any differentiation inducers; this has not been reported before. Four types of the substrate were newly established: 2D plasma-treated PCL (2D-TP), 2D non-plasma-treated PCL (2D-NP), 3D plasma-treated PCL (3D-TP), and 3D non-plasma-treated PCL (3D-NP). Physicochemical characterization revealed that only plasma-treated PCL scaffolds significantly increased the hydrophilicity and % oxygen/carbon ratio on the surfaces. The RMS roughness of 3D was higher than 2D conformation, whilst the plasma-treated surfaces were rougher than the non-plasma treated ones. The cytocompatibility test demonstrated that the 2D PCLs enhanced the initial cell attachment in comparison to the 3Ds, indicated by a higher expression of focal adhesion kinase. Meanwhile, the 3Ds promoted cell proliferation and migration as evidence of higher cyclin-A expression and filopodial protrusion, respectively. The 3Ds potentially protected the cell from apoptosis/necrosis but also altered the pluripotency/differentiation-related gene expression. In summary, the different configuration and surface properties of PCL scaffolds displayed the significant potential and effectiveness for facilitating stem cell growth and differentiation in vitro. The cell–substrate interactions on modified surface PCL may provide some information which could be further applied in substrate architecture for stem cell accommodation in cell delivery system for tissue repair.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Inthanon

Janvikul

Ongchai

et al. 2019

JFB

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show abstract

“…It is speculated that the surface of the 2D scaffolds provided anchorage points for initial attachment to hold the cell body in place, allowing subsequent filopodial protrusion to regulate cell shape and further growth [34]. Indeed, more numerous filopodial structures on 2D PCL scaffolds has been shown to promote greater cell attachment [35,36]. This result was further verified by the increased FAK expression described here, as filopodial shafts contain signaling pathways (e.g.…”

Section: Discussionsupporting

confidence: 52%

Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells on oxygen Plasma-Modified 2D and 3D Polycaprolactone Scaffolds

Inthanon¹,

Wongkham²,

Junwikul³

et al. 2018

Preprint

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Cell-based therapies and tissue engineering applications require biocompatible substrates that support and regulate the growth, survival, and differentiation of specific cell types. Extensive research efforts in regenerative medicine are devoted to the development of tunable biomaterials which support various cell types including stem cells. In this research, the non-cytotoxic biopolymer polycaprolactone (PCL) was fabricated into 2D and 3D scaffolds with or without the low-pressure oxygen plasma treatment to enhance hydrophilicity. Cellular responses and biocompatibility were evaluated using a human Wharton’s jelly mesenchymal stem cell line (BCP-K1). The 2D PCL scaffolds enhanced initial cell attachment compared to the 3Ds indicated by a higher expression of focal adhesion kinase (FAK). Whilst, the 3D scaffolds promoted cell proliferation and migration as evidenced by higher cyclin A expression and filopodial protrusion, respectively. The 3D scaffolds potentially protected the cell entering to apoptosis/necrosis program and induced cell differentiation, evaluated by gene expression. Both 2D and 3D PCL appeared to have stronger effects on cell behavior than a control substrate (polystyrene). In summarize, the different configuration and surface properties of PCL scaffolds provide various options for modulation of stem cell behaviors, including attachment, proliferation, survival, and differentiation, when combined with specific growth factors and culture conditions.

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“…More cells adhered to the surface of the PLGA-HACC membranes, and roughness of the PLGA-HACC surface might have contributed to these results. Several other studies have shown that cell response is improved by rough material surfaces [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Hydroxypropyltrimethyl Ammonium Chloride Chitosan Functionalized-PLGA Electrospun Fibrous Membranes as Antibacterial Wound Dressing: In Vitro and In Vivo Evaluation

et al. 2017

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Abstract:A novel poly(lactic-co-glycolic acid) (PLGA)-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) composite nanofiber wound dressing was prepared through electrospinning and the entrapment-graft technique as an antibacterial dressing for cutaneous wound healing. HACC with 30% degrees of substitution (DS) was immobilized onto the surface of PLGA membranes via the reaction between carboxyl groups in PLGA after alkali treatment and the reactive groups (-NH 2 ) in HACC molecules. The naked PLGA and chitosan graft PLGA (PLGA-CS) membranes served as controls. The surface immobilization was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared (FTIR), thermogravimetric analysis (TGA) and energy dispersive X-ray spectrometry (EDX). The morphology studies showed that the membranes remain uniform after the immobilization process. The effects of the surface modification by HACC and CS on the biological properties of the membranes were also investigated. Compared with PLGA and PLGA-CS, PLGA-HACC exhibited more effective antibacterial activity towards both Gram-positive (S. aureus) and Gram-negative (P. aeruginosa) bacteria. The newly developed fibrous membranes were evaluated in vitro for their cytotoxicity using human dermal fibroblasts (HDFs) and human keratinocytes (HaCaTs) and in vivo using a wound healing mice model. It was revealed that PLGA-HACC fibrous membranes exhibited favorable cytocompatibility and significantly stimulated adhesion, spreading and proliferation of HDFs and HaCaTs. PLGA-HACC exhibited excellent wound healing efficacy, which was confirmed using a full thickness excision wound model in S. aureus-infected mice. The experimental results in this work suggest that PLGA-HACC is a strong candidate for use as a therapeutic biomaterial in the treatment of infected wounds.

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PIII-induced enhancement and inhibition of human cell attachment on chitosan membranes

Cited by 12 publications

References 24 publications

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells on oxygen Plasma-Modified 2D and 3D Polycaprolactone Scaffolds

Hydroxypropyltrimethyl Ammonium Chloride Chitosan Functionalized-PLGA Electrospun Fibrous Membranes as Antibacterial Wound Dressing: In Vitro and In Vivo Evaluation

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PIII-induced enhancement and inhibition of human cell attachment on chitosan membranes

Cited by 12 publications

References 24 publications

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds

Growth and Differentiation of Human Wharton&rsquo;s Jelly Mesenchymal Stem Cells on oxygen Plasma-Modified 2D and 3D Polycaprolactone Scaffolds

Hydroxypropyltrimethyl Ammonium Chloride Chitosan Functionalized-PLGA Electrospun Fibrous Membranes as Antibacterial Wound Dressing: In Vitro and In Vivo Evaluation

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Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells on oxygen Plasma-Modified 2D and 3D Polycaprolactone Scaffolds