Hydroxypropyltrimethyl Ammonium Chloride Chitosan Functionalized-PLGA Electrospun Fibrous Membranes as Antibacterial Wound Dressing: In Vitro and In Vivo Evaluation

Abstract

Abstract:A novel poly(lactic-co-glycolic acid) (PLGA)-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) composite nanofiber wound dressing was prepared through electrospinning and the entrapment-graft technique as an antibacterial dressing for cutaneous wound healing. HACC with 30% degrees of substitution (DS) was immobilized onto the surface of PLGA membranes via the reaction between carboxyl groups in PLGA after alkali treatment and the reactive groups (-NH 2 ) in HACC molecules. The naked PLGA and chitosan graft PLGA (PLGA-CS) membranes served as controls. The surface immobilization was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared (FTIR), thermogravimetric analysis (TGA) and energy dispersive X-ray spectrometry (EDX). The morphology studies showed that the membranes remain uniform after the immobilization process. The effects of the surface modification by HACC and CS on the biological properties of the membranes were also investigated. Compared with PLGA and PLGA-CS, PLGA-HACC exhibited more effective antibacterial activity towards both Gram-positive (S. aureus) and Gram-negative (P. aeruginosa) bacteria. The newly developed fibrous membranes were evaluated in vitro for their cytotoxicity using human dermal fibroblasts (HDFs) and human keratinocytes (HaCaTs) and in vivo using a wound healing mice model. It was revealed that PLGA-HACC fibrous membranes exhibited favorable cytocompatibility and significantly stimulated adhesion, spreading and proliferation of HDFs and HaCaTs. PLGA-HACC exhibited excellent wound healing efficacy, which was confirmed using a full thickness excision wound model in S. aureus-infected mice. The experimental results in this work suggest that PLGA-HACC is a strong candidate for use as a therapeutic biomaterial in the treatment of infected wounds.

Polysaccharide-Based Formulations for Healing of Skin-Related Wound Infections: Lessons from Animal Models and Clinical Trials

“…PLGA-HACC induced a faster contraction of S. aureus-infected wounds than the other tested membranes (PLGA and PLGA-CS). This effect was confirmed by the lower levels of bacteria in skin (total eradication was observed after seven days of treatment) and higher collagen deposition [71].…”

Plant- vs. Bacterial-Derived Cellulose for Wound Healing: A Review

“…Apart from BC and PC, other products such as hydrogels, poly lactic-co-glycolic acid (PLGA), and 3D living constructs are being studied for their effectiveness in wound healing [ 99 , 100 , 101 , 102 ]. In current era, 3D bioprinting gaining much attention as other available biomaterial due to its ability to design cell components in microarchitecture.…”

Electrospun Chitosan/Poly (Vinyl Alcohol)/Graphene Oxide Nanofibrous Membrane with Ciprofloxacin Antibiotic Drug for Potential Wound Dressing Application

“…Indicative concretely, the wound dressing should possess excellent biomedical properties such as great cytocompatibility, non-toxicity, good antimicrobial activity, sufficient physical protection of the wound site against external intrusions, high porosity to allow for gas exchange and promotion of wound healing [2]. To date, substantial efforts have been made in various production techniques and materials available for the preparation of wound dressings [3]. Electrospun nanofibers, possessing a nanometer scale, are considered as the excellent wound dressing substrates due to the structural similarity to the extracellular matrix, which promote the cell adhesion, migration and proliferation [4].…”