Pigment-dispersing factor sets the night state of the medulla bilateral neurons in the optic lobe of the cricket, Gryllus bimaculatus

Saifullah, Asm; Tomioka, Kenji

doi:10.1016/s0022-1910(02)00270-6

Cited by 49 publications

(30 citation statements)

References 28 publications

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“…For light microscopy, Canton-S flies were anesthetized with CO 2 and fixed in aqueous Bouin's, and Paraplast wax sections 10-m thick were immunolabeled with a rabbit polyclonal antiserum raised against Gryllus-PDF, as the synthetic peptide NSEIINSLLGLPKVLNDA (Singaravel et al, 2003;Saifullah and Tomioka, 2003). In control characterization experiments this antibody did not immunolabel tissue from pdf 0 null flies, and omission of the primary antibody also eliminated positive immunolabeling of the sections.…”

Section: Immunocytochemistry For Light Microscopymentioning

confidence: 99%

Synaptic connections of PDF‐immunoreactive lateral neurons projecting to the dorsal protocerebrum of Drosophila melanogaster

Yasuyama

Meinertzhagen

2009

J of Comparative Neurology

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Recent studies in Drosophila melanogaster indicate that the neuropeptide pigment-dispersing factor (PDF) is an important output signal from a set of major clock neurons, s-LN(v)s (small ventral lateral neurons), which transmit the circadian phase to subsets of other clock neurons, DNs (dorsal neurons). Both s-LN(v)s and DNs have fiber projections to the dorsal protocerebrum of the brain, so that this area is a conspicuous locus for coupling between different subsets of clock neurons. To unravel the neural circuits underlying the fly's circadian rhythms, we examined the detailed subcellular morphology of the PDF-positive fibers of the s-LN(v)s in the dorsal protocerebrum, focusing on their synaptic connections, using preembedding immunoelectron microscopy. To examine the distribution of synapses, we also reconstructed the three-dimensional morphology of PDF-positive varicosities from fiber profiles in the dorsal protocerebrum. The varicosities contained large dense-core vesicles (DCVs), and also numerous small clear vesicles, forming divergent output synapses onto unlabeled neurites. The DCVs apparently dock at nonsynaptic sites, suggesting their nonsynaptic release. In addition, a 3D reconstruction revealed the presence of input synapses onto the PDF-positive fibers. These were detected less frequently than output sites. These observations suggest that the PDF-positive clock neurons receive neural inputs directly through synaptic connections in the dorsal protocerebrum, in addition to supplying dual outputs, either synaptic or via paracrine release of the DCV contents, to unidentified target neurons.

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Section: Immunocytochemistry For Light Microscopymentioning

confidence: 99%

Synaptic connections of PDF‐immunoreactive lateral neurons projecting to the dorsal protocerebrum of Drosophila melanogaster

Yasuyama

Meinertzhagen

2009

J of Comparative Neurology

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“…In the blowfly showing a similar pattern of rhythmic changes in the size of the lamina monopolar cells, as observed in the housefly, injected PDF mimics the night fashion of ERG rhythm supporting PDF release at the end of day (Chen et al, 1999;Pyza and Cymborowski, 2001). In the optic lobe of cricket, endogenous PDF seems to be released and bind to its receptors at night to set the night state of the medulla bilateral neurons (Saifullah and Tomioka, 2003).…”

Section: Cycling Of Pdf In Pdh-ir Neuronsmentioning

confidence: 99%

Circadian release of pigment‐dispersing factor in the visual system of the housefly, Musca domestica

Miśkiewicz

Schürmann

Pyza

2008

J of Comparative Neurology

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In the present study we examined profiles of nerve fiber varicosities containing dense core vesicles (DCVs) in the distal medulla of the housefly's optic lobe using electron microscopic methods. These profiles are infrequent among other neuronal profiles and do not contain presynaptic specializations for the release of DCVs. Presynaptic elements surrounded by electron-translucent vesicles were only occasionally detected, whereas synaptic input sites to the profiles containing DCVs were never observed. Among the varicosities in the distal medulla, those immunoreactive to pigment-dispersing hormone (PDH) are most numerous. The DCVs of PDH-immunoreactive (PDH-ir) varicosities differ by size from DCVs of other profiles. Moreover, in the day/night cycle PDH-ir varicosities show differences in structure revealing the rhythmic accumulation and release of PDF. There were fewer PDH-ir DCV per varicosity profile in flies fixed 1 hour after lights-on than in flies fixed 1 hour after lights-off. Moreover, at the beginning of the day all DCVs harbored an electron-dense matrix, while at the beginning of the night numerous electron-lucent DCVs were observed. By applying a bath stimulation with a high potassium concentration we also showed that depolarizing events are involved in peptide release in the medulla. After potassium treatment immunolabeling with anti-PDH serum was weaker and PDH-ir varicosities were smaller and more distant from each other than in control animals.

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“…Our results showed that PDF did not enhance significantly the locomotor activity, except at CT 4. Since microinjection of PDF into the optic lobe during the day increased the spontaneous activity of the brain efferent (Saifullah and Tomioka, 2003), the enhancement of activity by PDF at CT4 may be related to the increased neuronal activity. However, considering that non-consistent enhancement was induced by PDF injections at other CTs, the increased neuronal activity is not positively related with the overt locomotor activity.…”

Section: Functional Role Of Pdfmentioning

confidence: 99%

Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

Singaravel¹,

Fujisawa²,

Hisada³

et al. 2003

Zoological Science

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-Pigment-dispersing factors (PDFs) are octadeca-peptides widely distributed in insect optic lobes and brain. In this study, we have purified PDF and determined its amino acid sequence in the cricket Gryllus bimaculatus . Its primary structure was NSEIINSLLGLPKVLNDA-NH 2 , homologous to other PDH family members so far reported. When injected into the optic lobe of experimentally blinded adult male crickets, Gryllus -PDF induced phase shifts in their activity rhythms in a phase dependent and dose dependent manner. The resulted phase response curve (PRC) showed delays during the late subjective night to early subjective day and advances during the mid subjective day to mid subjective night. The PRC was different in shape from those for light, serotonin and temperature. These results suggest that PDF plays a role in phase regulation of the circadian clock through a separate pathway from those of other known phase regulating agents.

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Pigment-dispersing factor sets the night state of the medulla bilateral neurons in the optic lobe of the cricket, Gryllus bimaculatus

Cited by 49 publications

References 28 publications

Synaptic connections of PDF‐immunoreactive lateral neurons projecting to the dorsal protocerebrum of Drosophila melanogaster

Synaptic connections of PDF‐immunoreactive lateral neurons projecting to the dorsal protocerebrum of Drosophila melanogaster

Circadian release of pigment‐dispersing factor in the visual system of the housefly, Musca domestica

Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

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