Circadian release of pigment‐dispersing factor in the visual system of the housefly, <i>Musca domestica</i>

Miśkiewicz, Katarzyna; Schürmann, Friedrich‐Wilhelm; Pyza, Elżbieta

doi:10.1002/cne.21765

Cited by 15 publications

(13 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As these are putative PDF release sites, this may represent sites of local presynaptic regulation of PDF release. In houseflies, similar optic lobe varicosities show reduced PDF levels after lights on [34]. In Drosophila these terminals appeared to show a similar trend toward lower levels after lights on[35].…”

Section: Resultsmentioning

confidence: 99%

The GABAA Receptor RDL Acts in Peptidergic PDF Neurons to Promote Sleep in Drosophila

et al. 2009

View full text Add to dashboard Cite

SUMMARY Sleep is regulated by a circadian clock that largely times sleep and wake to occur at specific times of day and a sleep homeostat that drives sleep as a function of duration of prior wakefulness[1]. To better understand the role of the circadian clock in sleep regulation, we have been using the fruit fly Drosophila melanogaster[2]. Fruit flies display all of the core behavioral features of sleep including relative immobility, elevated arousal thresholds and homeostatic regulation[2, 3]. We assessed sleep-wake modulation by a core set of 20 circadian pacemaker neurons that express the neuropeptide PDF. We find that PDF neuron ablation, loss of pdf or its receptor pdfr results in increased sleep during the late night in light:dark (LD) conditions and more prominent increases on the first subjective day of constant darkness (DD). Flies deploy similar genetic and neurotransmitter pathways to regulate sleep as their mammalian counterparts, including GABA[4]. We find that RNAi-mediated knockdown of the GABAA receptor gene, Resistant to dieldrin (Rdl), in PDF neurons, reduced sleep consistent with a role for GABA in inhibiting PDF neuron function. Patch clamp electrophysiology reveals GABA-activated picrotoxin-sensitive chloride currents on PDF+ neurons. In addition, RDL is detectable most strongly on the large subset of PDF+ pacemaker neurons. These results suggest that GABAergic inhibition of arousal promoting PDF neurons is an important mode of sleep-wake regulation in vivo.

show abstract

Section: Resultsmentioning

confidence: 99%

The GABAA Receptor RDL Acts in Peptidergic PDF Neurons to Promote Sleep in Drosophila

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Their terminals in the lamina and medulla show circadian structural changes [4] and cyclically release PDF [29] that affects target neurons and/or glial cells. In D. melanogaster circadian information from the central pacemaker neurons to the visual system are probably transmitted to per -expressing glial cell in the medulla.…”

Section: Discussionmentioning

confidence: 99%

Circadian Control of Dendrite Morphology in the Visual System of Drosophila melanogaster

2009

Self Cite

View full text Add to dashboard Cite

BackgroundIn the first optic neuropil (lamina) of the fly's visual system, monopolar cells L1 and L2 and glia show circadian rhythms in morphological plasticity. They change their size and shape during the day and night. The most pronounced changes have been detected in circadian size of the L2 axons. Looking for a functional significance of the circadian plasticity observed in axons, we examined the morphological plasticity of the L2 dendrites. They extend from axons and harbor postsynaptic sites of tetrad synaptic contacts from the photoreceptor terminals.Methodology/Principal FindingsThe plasticity of L2 dendrites was evaluated by measuring an outline of the L2 dendritic trees. These were from confocal images of cross sections of L2 cells labeled with GFP. They were in wild-type and clock mutant flies held under different light conditions and sacrified at different time points. We found that the L2 dendrites are longest at the beginning of the day in both males and females. This rhythm observed under a day/night regime (LD) was maintained in constant darkness (DD) but not in continuous light (LL). This rhythm was not present in the arrhythmic per01 mutant in LD or in DD. In the clock photoreceptor cryb mutant the rhythm was maintained but its pattern was different than that observed in wild-type flies.Conclusions/SignificanceThe results obtained showed that the L2 dendrites exhibit circadian structural plasticity. Their morphology is controlled by the per gene-dependent circadian clock. The L2 dendrites are longest at the beginning of the day when the daytime tetrad presynaptic sites are most numerous and L2 axons are swollen. The presence of the rhythm, but with a different pattern in cryb mutants in LD and DD indicates a new role of cry in the visual system. The new role is in maintaining the circadian pattern of changes of the L2 dendrite length and shape.

show abstract

“…It would be interesting to determine whether circadian changes in (say) secretory granule density in PDH-containing terminals or fluorescence of immunolabeled whole-mounted brains could be correlated with chromatophore dispersion. Circadian remodeling in the axon terminals of the PDF circuit has been observed in D. melanogaster (Fernandez et al, 2008), and changes in the morphology of PDF-containing neurites (varicosities and dense-core vesicles) have been reported in Musca domestica (Pyza and Meinertzhagen, 1997;Miśkiewicz et al, 2008).…”

Section: Circadian Color Change Rhythms and Expression Of Pdh Mrnamentioning

confidence: 95%

“…Knockdown of PDF by RNAi abolishes circadian rhythmicity in Blatella (Lee et al, 2009), and injected peptide shifts the phase of the circadian pacemaker in the cockroach Leucophaea (Petri and Stengl, 1997). PDF is also involved in circadian morphological changes in the eyes of Musca domestica (Pyza and Meinertzhagen 1996, 1997, 1998Pyza, 2002), and some neurites of PDF-expressing neurons exhibit circadian changes in morphology (Pyza and Meinertzhagen, 1997;Miśkiewicz et al, 2008).…”

mentioning

confidence: 98%

A novel form of pigment‐dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach)

Wilcockson

Zhang

Hastings

et al. 2010

J of Comparative Neurology

View full text Add to dashboard Cite

Pigment-dispersing factor (PDF) is well known as a circadian clock output factor, which drives daily activity rhythms in many insects. The role of its homologue, pigment-dispersing hormone (PDH), in the regulation of circadian and/or circatidal rhythmicity in crustaceans is, however, poorly understood. The intertidal isopod crustacean, Eurydice pulchra has well-defined circatidal (12.4-hour) activity rhythms. In this study we show that this runs parallel to a circadian (24-hour) cycle of chromatophore dispersion. As a first step in determining the potential role of PDH in these rhythms, we have identified a novel form of PDH expressed in this species. Because conventional homology cloning was unsuccessful, we employed immuno-identification and Edman microsequencing to determine the primary structure of this peptide. From this, cDNA cloning identified the nucleotide encoding sequence and thus facilitated description of PDH neurons by in situ hybridization and immunohistochemistry. We show them to be morphologically similar to those that co-ordinate circadian activity rhythms in insects. In animals expressing both tidal (activity) and circadian (chromatophore) rhythms, however, there was no evidence for a corresponding periodicity in the expression of pdh transcript, as determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in Eurydice heads. It is therefore suggested that any role for PDH in daily/tidal timing in Eurydice is not mediated at the transcriptional level, rather rhythms in neurohemal release may be important in such co-ordination.

show abstract

Circadian release of pigment‐dispersing factor in the visual system of the housefly, Musca domestica

Cited by 15 publications

References 58 publications

The GABAA Receptor RDL Acts in Peptidergic PDF Neurons to Promote Sleep in Drosophila

The GABAA Receptor RDL Acts in Peptidergic PDF Neurons to Promote Sleep in Drosophila

Circadian Control of Dendrite Morphology in the Visual System of Drosophila melanogaster

A novel form of pigment‐dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach)

Contact Info

Product

Resources

About