Piglets born after non-surgical deep intrauterine transfer of vitrified blastocysts in gilts

Cuello, C.; Berthelot, F.; Martinat-Botté, F.; Venturi, Éric; Guillouet, Philippe; Vázquez, J.M.; Roca, Jordi; Martı́nez, Emilio A.

doi:10.1016/j.anireprosci.2004.04.039

Cited by 62 publications

(54 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the in vitro development of vitrified embryos after rewarming was nearly 100%. Therefore, it is likely that similar numbers of piglets could be obtained by transferring fewer embryos than in this study; in other words, a higher production efficiency of piglets could be achieved.Production of pigs from vitrified in vivo-derived embryos has been achieved using blastocysts [40][41][42][43][44]. Pig blastocysts at later stages are known to be more cryotolerant than early cleavage stage embryos [45][46][47][48].…”

mentioning

confidence: 99%

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Matsunari

Maehara

Nakano

et al. 2012

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. Current embryo vitrification methods with proven efficacy are based on the minimum volume cooling (MVC) concept by which embryos are vitrified and rewarmed ultrarapidly in a very small amount of cryopreserving solution to ensure the high viability of the embryos. However, these methods are not suitable for simultaneously vitrifying a large number of embryos. Here, we describe a novel vitrification method based on use of a hollow fiber device, which can easily hold as many as 40 mouse or 20 porcine embryos in less than 0.1 µl of solution. Survival rates of up to 100% were obtained for mouse embryos vitrified in the presence of 15% DMSO, 15% ethylene glycol and 0.5 M sucrose using the hollow fiber vitrification (HFV) method, regardless of the developmental stage of the embryos (1-cell, 2-cell, morula or blastocyst; n = 50/ group). The HFV method was also proven to be effective for vitrifying porcine in vitro-and in vivo-derived embryos that are known to be highly cryosensitive. For porcine embryos, the blastocyst formation rate of in vitro maturation (IVM)-derived parthenogenetic morulae after vitrification (48/65, 73.8%) did not decrease significantly compared with non-vitrified embryos (59/65, 90.8%). Transfer of 72 in vivo-derived embryos vitrified at the morula/early blastocyst stages to 3 recipients gave rise to 29 (40.3%) piglets. These data demonstrate that the HFV method enables simultaneous vitrification of multiple embryos while still adhering to the MVC concept, and this new method is very effective for cryopreserving embryos of mice and pigs. Key words: Cryopreservation, Hollow fiber, Mouse embryos, Pig embryos, Vitrification (J. Reprod. Dev. 58: [599][600][601][602][603][604][605][606][607][608] 2012) E mbryo cryopreservation is widely and routinely used both for research in reproductive biology and for the development of practical applications in animal industries and human reproductive medicine. The cryopreservation of oocytes and embryos is essential for the long-term preservation of valuable genetic resources in experimental and livestock animals [1][2][3][4][5], and the cryopreservation of embryos improves pregnancy rates in assisted reproductive technology applications [6][7][8]. Various protocols have been developed for the cryopreservation of animal embryos, and many are used in research and clinical applications [1,5,9].In recent years, embryo vitrification has been applied to an increasing number of animal species, and high post-cryopreservation embryo viability has been achieved [10,11]. The basic concept of vitrification is as follows: a solution containing a high concentration of a cryoprotective agent (CPA) is rapidly cooled, causing it to transform from the liquid phase to the solid phase without forming ice crystals. As a result, cells suspended in the solution are preserved in an ultra-low temperature glassy (amorphous) material [10,12]. To create this glassy state, a solution containing a high concentration of CPA (normally 4-6 M) needs to be cooled ultrarapidly. Howeve...

show abstract

mentioning

confidence: 99%

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Matsunari

Maehara

Nakano

et al. 2012

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

“…Several studies on non-surgical embryo transfer in pigs have been reported [9][10][11][12][13], and we have also been working on the development of a non-surgical transfer technique that uses a strawattached intrauterine injector [14]. Direct transfer via a straw containing vitrified embryos with high viability may be applied to piglet production by non-surgical embryo transfer in an ordinary commercial farm.…”

mentioning

confidence: 99%

Viability of Porcine Embryos after Vitrification Using Water-soluble Pullulan Films

Sakagami¹,

Yamamoto²,

Akiyama³

et al. 2010

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. The efficiency of a porcine embryo vitrification method that uses water-soluble films of pullulan, a naturallyoccurring polysaccharide polymer, was compared with two other types of vitrification methods using different devices and solutions for vitrification and warming. Blastocysts collected in vivo and vitrified by the conventional straw (ST), Cryotop ® (MVC) or pullulan film vitrification (PFV) methods were stored in liquid nitrogen for a certain period of time, after which the cryoprotective agents were removed by stepwise dilution. Fresh embryos were used as controls for the non-vitrification group. The vitrified-warmed embryos were incubated in TCM199 with 0.1 mM β-mercaptoethanol and 20% fetal bovine serum for 24 h at 38.5 C in humidified air with 5% CO2 to evaluate their viability. The survival rate of embryos in the ST group (48.3%) was significantly lower than that of those in the MVC (70.7%), PFV (79.0%) and nonvitrification (94.4%) groups. The oxygen consumption rate after vitrification was significantly lower than that before vitrification in the ST group, but was not significantly different in the MVC and PFV groups. Both the oxygen consumption rates of embryos after warming and the live cell numbers in the ST group were lower than those in the MVC group, while they did not differ significantly between the PFV and MVC groups. There was a correlation between the oxygen consumption rate and the number of live cells in vitrified embryos after warming. Our results demonstrated that in vivo-derived porcine embryos could be vitrified using pullulan films. Key words: Oxygen consumption rate, Porcine embryo, Pullulan film, Vitrification (J. Reprod. Dev. 56: [279][280][281][282][283][284] 2010) reservation of porcine embryos is important for increasing the effective use of high-quality genetic resources, preventing disease transmission via animals and allowing low-cost transportation of pigs. Due to the extremely high sensitivity of porcine embryos to low temperature, it has been difficult with slow freezing methods to achieve conception rates and litter sizes equivalent to those achieved with artificial insemination [1,2]. However, it has recently become possible to produce piglets from cryopreserved embryos by using ultra-rapid vitrification methods, such as the minimum volume cooling (MVC) [3,4], open pulled straw [5], microdroplet [6] and metal mesh vitrification [7] methods.Pullulan, which is a neutral polysaccharide polymer also known as α-1,4-; α-1,6-glucan, is made from starch and consists of maltotriose units linked in an orderly manner. It has been reported that murine morulae placed on a pullulan film could be vitrified [8]. In general, stepwise dilution is required after warming of vitrified embryos, since vitrification requires a high concentration of cryoprotective agents (CPAs), which makes it difficult, due to their toxicity, to warm the embryos in a straw for direct transfer to recipients. As the pullulan film is soluble in warm water, the vitrification solution can be diluted ...

show abstract

“…Several reasons justify this decision. Firstly, the percentages of overall catheter insertions and correct insertions are higher, there is less difficulty in catheter insertion, and the behavior during catheter insertions is better in sows than in gilts [1,18]. Secondly, the use of recipient sows was preferred because weaning is a physiological, regular and outstanding process that facilitates estrus synchronization in swine [19].…”

Section: Discussionmentioning

confidence: 99%

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Piglets born after non-surgical deep intrauterine transfer of vitrified blastocysts in gilts

Cited by 62 publications

References 20 publications

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Viability of Porcine Embryos after Vitrification Using Water-soluble Pullulan Films

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

Contact Info

Product

Resources

About