Pig Cloning Using Somatic Cell Nuclear Transfer

Ouyang, Hongsheng; Han, Jianyong; Huang, Yongye

doi:10.1007/978-1-0716-1084-8_1

Cited by 6 publications

(5 citation statements)

References 67 publications

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“…Somatic cell nuclear transfer (SCNT) is of much current interest with potential applications in the protection of endangered species, agriculture, and regenerative medicine ( Wu and Hochedlinger, 2011 ; Tachibana et al, 2013 ; Iqbal et al, 2021 ; Son et al, 2021 ). So far, it has successfully produced offspring in pigs, monkeys, goat, and other animals ( Hajian et al, 2020 ; Ouyang et al, 2021 ; Sun et al, 2021 ). SCNT can reprogram terminally differentiated somatic cells to the totipotent state when transplanted into enucleated mature oocytes (metaphase II, MII) ( Gonzalez-Munoz and Cibelli, 2018 ), but its mechanisms remain unclear ( Keefer, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

The Role of Ca2 + in Maturation and Reprogramming of Bovine Oocytes: A System Study of Low-Calcium Model

Meng

Hao

Liu

et al. 2021

Front. Cell Dev. Biol.

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[Ca2+]i is essential for mammalian oocyte maturation and early embryonic development, as those processes are Ca2+ dependent. In the present study, we investigated the effect of [Ca2+]i on in vitro maturation and reprogramming of oocytes in a lower calcium model of oocyte at metaphase II (MII) stage, which was established by adding cell-permeant Ca2+ chelator BAPTA-AM to the maturation medium. Results showed that the extrusion of the first polar body (PB1) was delayed, and oocyte cytoplasmic maturation, including mitochondrial and endoplasmic reticulum distribution, was impaired in lower calcium model. The low-calcium-model oocytes presented a poor developmental phenotype of somatic cell nuclear transfer (SCNT) embryos at the beginning of activation of zygotic genome. At the same time, oxidative stress and apoptosis were observed in the low-calcium-model oocytes; subsequently, an RNA-seq analysis of the lower-calcium-model oocytes screened 24 genes responsible for the poor oocyte reprogramming, and six genes (ID1, SOX2, DPPA3, ASF1A, MSL3, and KDM6B) were identified by quantitative PCR. Analyzing the expression of these genes is helpful to elucidate the mechanisms of [Ca2+]i regulating oocyte reprogramming. The most significant difference gene in this enriched item was ID1. Our results showed that the low calcium might give rise to oxidative stress and apoptosis, resulting in impaired maturation of bovine oocytes and possibly affecting subsequent reprogramming ability through the reduction of ID1.

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Section: Introductionmentioning

confidence: 99%

The Role of Ca2 + in Maturation and Reprogramming of Bovine Oocytes: A System Study of Low-Calcium Model

Meng

Hao

Liu

et al. 2021

Front. Cell Dev. Biol.

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“…The emergence of somatic cell nuclear transfer (SCNT) and reliable genome editing techniques make possible for the generation of porcine models of human diseases. The efficacy of porcine SCNT has been largely improved and several kinds of small molecular inhibitors have been applied to enhance the developmental competence of SCNT embryos ( Ouyang et al, 2021 ; Hou et al, 2022 ). CRISPR/Cas9-mediated gene editing strategies are widely used in the establishment of porcine models of human diseases.…”

Section: Pigmentioning

confidence: 99%

Editorial: Applying large animals for developmental study and disease modeling

Lin

Feng

Kubiak

et al. 2023

Front. Cell Dev. Biol.

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“…La transferencia nuclear de células somáticas (SCNT, somatic cell nuclear transfer) implica transferir el núcleo de una célula somática a un oocito enucleado, produciéndose la reprogramación epigenética de la célula donante y generándose un nuevo embrión, que es transferido a una hembra receptora pseudogestante en la que tendrá lugar la gestación (Polejaeva, 2021). Los fibroblastos fetales porcinos son las células más utilizadas como donantes de núcleo (Ouyang et al, 2021); estas células se pueden modificar genéticamente en cultivo para, posteriormente, generar animales modificados genéticamente mediante SCNT.…”

Section: Metodologías Para La Generación De Cerdos Destinados a Xenot...unclassified

Modificación genética en cerdos destinados a xenotrasplante

2022

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La escasez de órganos humanos para trasplante ha propiciado la búsqueda de otras alternativas, como la posibilidad de utilizar animales como donantes, lo que se conoce como xenotrasplante. El cerdo se ha convertido en la especie donante más prometedora tanto por su similitud anatómica y fisiológica con el ser humano, como por la disponibilidad de metodologías de modificación genética, que han permitido la obtención de modelos porcinos que permiten hacer frente a los riesgos del xenotrasplante. Estos riesgos están asociados con el rechazo inmunológico, la desregulación de la coagulación y la presencia de retrovirus endógenos porcinos. Aunque los resultados de los ensayos preclínicos en primates varían considerablemente, alcanzando diferentes tiempos de supervivencia en función del órgano trasplantado, la inactivación del gen que codifica el antígeno α-Gal ha resultado imprescindible para superar el rechazo hiperagudo, que es la primera y más drástica barrera inmunológica. Los hitos que se han logrado en los últimos años, incluyendo las pruebas clínicas recientes con seres humanos a los que se han realizado trasplantes de riñón o corazón de cerdos modificados genéticamente, acercan un paso más el traslado de los xenotrasplantes a la práctica clínica

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Pig Cloning Using Somatic Cell Nuclear Transfer

Cited by 6 publications

References 67 publications

The Role of Ca2 + in Maturation and Reprogramming of Bovine Oocytes: A System Study of Low-Calcium Model

The Role of Ca2 + in Maturation and Reprogramming of Bovine Oocytes: A System Study of Low-Calcium Model

Editorial: Applying large animals for developmental study and disease modeling

Modificación genética en cerdos destinados a xenotrasplante

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