Piezoelectric immuno sensor for the detection of candida albicans microbes

Magnetic‐Core/Porous‐Shell CoFe2O4/SiO2 Composite Nanoparticles as Immobilized Affinity Supports for Clinical Immunoassays

“…The quartz crystal microbalance (QCM) immunoassay technique offers some key advantages, such as high sensitivity, low cost, real-time output, and label-or radiation-free entities, and thus has been the subject of extensive investigation for use in clinical bioassays and for studying biomolecular interactions. [22,23] However, the immobilization of biomolecules is vital to the successful development of QCM assay systems. In the present paper, we have used the physical characteristics of the nanometer-sized magnetic cores and the good biocompatibility of the silica shells to construct a nontoxic biomimetic interface for the immobilization of CA 15-3 antibodies; this approach provides an environment similar to the native system and allows more freedom of orientation for the biomolecules, thus efficiently retaining the bioactivity of the proteins.…”

QCM as Cell-Based Biosensor

“…The first reported gas phase biosensor was in fact an enzyme-based Pz sensor (Guilbault, 1983). The first Pz immunosensor for microbial pathogens was developed by Muramatsu et al in 1986(Muramatsu et al, 1986. Their Pz crystals were coated with antibodies against Candida albicans.…”

“…Guilbault reported an enzyme-based QCM sensor in gas phase (Guilbault, 1983). A quartz immunosensor for microbial pathogens was developed by Muramatsu et al (Muramatsu et al, 1986). Since then, liquid QCM began to be applied to cell biology, biotechnology, immunology and electrochemistry.…”

Die Anwendung von Viren in Chemo‐ und Biosensoren

“…[71] QCMSensoren können dazu dienen, die Bindung zwischen einem Analyt und einer Sonde zu verfolgen, die an der Oberfläche eines piezoelektrischen Kristalls verankert ist. Solche QCMSensoren wurden für Immunassays [72,73] sowie zum Nachweis von Bakterien, [74][75][76][77][78][79][80][81][82][83] Viren und Toxinen entwickelt. [82,[84][85][86] Der gravierendste Nachteil dieser Methode ist die mangelnde Selektivität.…”