Piezoelectric Flow Injection Analysis Biosensor for the Detection of <i>Salmonella Typhimurium</i>

Babacan, Sibel; Pivarnik, P.; Letcher, S. V.; Rand, Arthur G.

doi:10.1111/j.1365-2621.2002.tb11403.x

Cited by 39 publications

(11 citation statements)

References 16 publications

(6 reference statements)

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“…Doseresponse curves were created for spiked buffer samples to assess the MAAB's overall performance and to provide a basis for comparison with other so-called rapid methods of detection. The LODs determined here for both 15-min and 1-h protocols (ϳ10 4 CFU/ml) compare favorably with those for several other antibody-based systems (LODs in the range of mid-10 3 to 10 5 CFU/ml), including piezoelectric sensors (4,33,43,45,59), fiber optic sensors (44,71,72), an interferometer (57), surface plasmon resonance-based sensors (9,56), and an electrochemical detector (22). ORIGEN, a system based on electrochemiluminescence, has proven to be the most sensitive rapid detection system, with a detection limit of 1,000 CFU/ml (70).…”

Section: Discussionsupporting

confidence: 55%

“…We have also recently tested Listeria monocytogenes and Shigella dysenteriae in assays for Salmonella and again observed only minimal levels of nonspecific binding (K. Sapsford and N. Kulagina, personal communications). Other detection systems have shown a similar overall lack of interference by the presence of nonrelevant bacteria (4,12,57,59). Although we did not attempt to assess the specificity of the system using other Salmonella species, this method should prove amenable to detection of other salmonellae, provided that a more broadly reactive tracer antibody of similar affinity is used.…”

Section: Discussionmentioning

confidence: 71%

See 1 more Smart Citation

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Taitt

Shubin

Angel

et al. 2004

Appl Environ Microbiol

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The multianalyte array biosensor (MAAB) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. Rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of Salmonella enterica serovar Typhimurium, with a detection limit of 8 ؋ 10 4 CFU/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. S. enterica serovar Typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparation: sausage, cantaloupe, whole liquid egg, alfalfa sprouts, and chicken carcass rinse. Cross-reactivity tests were performed with Escherichia coli and Campylobacter jejuni. To determine whether the MAAB has potential as a screening tool for the diagnosis of asymptomatic Salmonella infection of poultry, chicken excretal samples from a private, noncommercial farm and from university poultry facilities were tested. While the private farm excreta gave rise to signals significantly above the buffer blanks, none of the university samples tested positive for S. enterica serovar Typhimurium without spiking; dose-response curves of spiked excretal samples from university-raised poultry gave limits of detection of 8 ؋ 10 3 CFU/g.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 71%

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Taitt

Shubin

Angel

et al. 2004

Appl Environ Microbiol

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“…or specific serotypes in a variety of food (3,8,13,18,21,25). Other authors developed immunoelectrochemical assays or piezoelectric flow injection analysis (FIA) biosensors to detect Salmonella enterica serotype Typhimurium (2,7,26). However, although these biosensors have the potential to provide direct label-free detection of bacteria, these piezoelectric FIA systems have a low level of sensitivity, and neither has been applied to food samples.…”

mentioning

confidence: 99%

Comparison of PCR, Electrochemical Enzyme-Linked Immunosorbent Assays, and the Standard Culture Method for Detecting Salmonella in Meat Products

Croci

Delibato

Volpe

et al. 2004

Appl Environ Microbiol

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An electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with flow injection analysis (ELISA-FIA) and a PCR-based method using ST11 and ST15 primers for detecting salmonellae in meat were evaluated in comparison with the International Organization for Standardization (ISO) culture method. The methods were applied to experimentally contaminated and naturally contaminated meat samples. The results showed that both ELISA-FIA and PCR allowed detection of salmonella in a product contaminated with a low number of the microorganisms (1 to 10 salmonellae/25 g) after only 5 h of incubation of preenrichment broth, and they were just as effective as the ISO method.The incidence of foodborne infection by Salmonella continues to be an important problem in the United States (16) and in the European Community (4, 16).Raw and undercooked meat, eggs, milk, and especially poultry are the most commonly implicated vehicles of Salmonella spp. infection (10,15,16,20,24). For foods to be considered "salmonella free," European legislation requires that from 1 to 25 g be tested, depending on the type of food. The standard culture method (ISO 6579/2002) for detection of salmonella in food is rather sensitive and quite inexpensive, but it requires 4 to 5 days to generate results.Given that efforts to prevent salmonellosis must be applied at all phases of the food production chain, based on the Hazard Analysis Critical Control Point (HACCP) system (23), the methods for detecting salmonella need to be more rapid than the ISO method. To this end, new technologies have been used to develop rapid methods. Specifically, a number of antibodyantigen-based methods have been developed to detect Salmonella spp. or specific serotypes in a variety of food (3,8,13,18,21,25). Other authors developed immunoelectrochemical assays or piezoelectric flow injection analysis (FIA) biosensors to detect Salmonella enterica serotype Typhimurium (2, 7, 26). However, although these biosensors have the potential to provide direct label-free detection of bacteria, these piezoelectric FIA systems have a low level of sensitivity, and neither has been applied to food samples.Methods based on PCR for the detection of salmonella in food have also been developed (12,17,19).All of the above methods require that a preenrichment step be performed (5,9,14,18,22).The objective of the present study was to evaluate two rapid techniques for detection of salmonella: an electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with FIA (ELISA-FIA) (6) and a PCR method using ST11 and ST15 primers (1).The experiments were first performed with experimentally contaminated samples to reduce and optimize the preenrichment times; the two methods were then applied to naturally contaminated meat samples, and the results were compared to those obtained with the International Organization for Standardization (ISO) method. MATERIALS AND METHODSThe experiments were performed on the following: (i) experimentally contaminated meat samples, to establish the minimum preenrichment...

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“…In this way, valuable characteristics of affinity binding reaction can be obtained easily and quickly. [123][124][125][126][127][128][129][130][131] …”

Section: Discussionmentioning

confidence: 99%

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

Skládal¹

2003

J. Braz. Chem. Soc.

109

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Esta revisão apresenta cristais piezoeletricos de quartzo como transductores aplicáveis no desenvolvimento de diferentes tipos de ensaios bioanaliticos. Os componentes do sistema de medidas para sensores tipo piezo são descritos juntamente com os sistemas de equipamentos comerciais existentes. Os biosensores piezo elétricos podem ser aplicados para determinação de vírus, bacteriais e outras células, proteínas, ácidos nucléicos e pequenas moléculas encontradas em remédios, hormônios e pesticidas. Além de mudança de massa, alguns ensaios de aglutinação empregando efeitos de viscosidade são discutidos. Finalmente, o monitoramento da afinidade das interações direto semmarcação e em tempo real usando sensores piezo é apresentado. Os pressupostos teóricos para a determinação apropriada das constantes de velocidade e de equilíbrio são apresentados e uma aproximação é demonstrada para a interação anticorpo com o correspondente antígeno (proteína secalin). Vários exemplos de estudo de afinidade são mostrados, incluindo a interação de proteínas (anticorpos e antígenos), de ácidos nucléicos (hibridização, intercalação de complexos metálicos), lipídeos e camadas com base de sacarídeos.This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

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Piezoelectric Flow Injection Analysis Biosensor for the Detection of Salmonella Typhimurium

Cited by 39 publications

References 16 publications

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Comparison of PCR, Electrochemical Enzyme-Linked Immunosorbent Assays, and the Standard Culture Method for Detecting Salmonella in Meat Products

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

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