Picoscope, a new label-free biosensor

Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications

“…Biacore measurements have recently expanded from four channels (Biacore T100) with imaging SPR technology, yet reported examples are still limited to a few hundred spots (17). Wavelength-dependent reflectance from layered surfaces has also been studied for optical detection of biomolecular interactions for many years (18)(19)(20)(21)(22)(23)(24). We argue that SRIB presents advantages when compared with these previous techniques.…”

“…Picometer noise floor was shown by several researchers (15,18), but, when imaging a multiplexed array is required, the reported sensitivity drops. Averaging improves sensitivity by increasing the total number of detected photons and can reduce the effects of surface roughness.…”

Synthetic peptide fragment (65–76) of monocyte chemotactic protein-1 (MCP-1) inhibits MCP-1 binding to heparin and possesses anti-inflammatory activity in stable angina patients after coronary stenting

“…The device was successfully tested for recording the dynamics of interactions between various protein molecules. Picoscope Ò permits quantitative registration of changes of average optical thickness of a molecular layer on a surface (i.e., the product of the refraction index n and the geometric thickness of the layer Dd) at picometer scale [27]. Microscopic glass slips used as sensor chips were silanized and then covered with biotin.…”

Design Principles for Microfluidic Biomedical Diagnostics in Space

“…Aside from fabrication challenges and gas permeability, another concern is the degradation of biochip components, such as electrodes Zhang et al, 2007), which may interfere with detection performance, release contaminants into the device, or affect containment. In some cases, electronics can be placed outside of the channel to prohibit contact between the sample and the sensor (Nikitin et al, 2005). Processes that employ electrical or magnetic fields to drive, mix or separate fluid streams can also be designed to avoid contact of the control elements with the sample.…”