Picomole assay for Nτ-methylhistidine by gas chromatography—Mass spectrometry

Matthews, Dwight E.; Starren, Justin; Drexler, Andrew; Kipnis, David; Bier, Dennis M.

doi:10.1016/0003-2697(81)90196-2

Cited by 20 publications

(1 citation statement)

References 22 publications

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“…Determination of plasma 3-MH was performed using deuterated 3-MH internal standards by gas chromatography-mass spectrometry as previously described [22]. Urine was deproteinized (Amicon, Inc., Lexington, MA, U.S.A.) and 3-MH was determined using column chromatography (Beckman Instruments, Fullerton, CA, U.S.A.) by previously described methods…”

Section: Analysis Of Blood and Urinementioning

confidence: 99%

Protein and substrate metabolism during starvation and parenteral refeeding

et al. 1988

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1. Healthy male volunteers underwent 10 days of hospitalized protein-calorie starvation and a subsequent 10 day repletion phase with complete intravenous nutritional support (IVF). Non-protein calories were provided as either all D-glucose or as 50% D-glucose/50% lipid. 2. In comparison with starvation, whole-body protein breakdown, as assessed by [15N]glycine, [13C]leucine and urinary excretion of 3-methylhistidine (3-MH), was diminished during IVF. The administration of parenteral nutrition did not specifically suppress peripheral tissue protein breakdown, as measured by extremity 3-MH efflux. 3. Despite the differential insulin response to D-glucose/amino acid (50 +/- 6 m-units/ml) as compared with the D-glucose/lipid/amino acid regimen (25 +/- 4 m-units/ml), there was no difference in nitrogen retention between the regimens. Indirect calorimetric determinations revealed that oxidation of substrate during IVF was related to the proportion of D-glucose and lipid infusion.

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Section: Analysis Of Blood and Urinementioning

confidence: 99%

Protein and substrate metabolism during starvation and parenteral refeeding

et al. 1988

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Gas chromatographic/mass spectrometric analysis of stable isotopes of 3-methylhistidine in biological fluids: Application to plasma kineticsin vivo

Rathmacher

Link

Flakoll

et al. 1992

Biol. Mass Spectrom.

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A simple and rapid method for measuring 3-methylhistidine (3MH) in plasma and urine is described. Internal standard, 1-methylhistidine (1MH), was added to plasma, acidified and absorbed onto cation-exchange columns. It was then eluted from columns, dried, and derivatized for gas chromatography/mass spectrometry. A major fragment of 3MH was monitored at 238 u and 3-methyl-(methyl-2H3)histidine (d3-3MH) (used for in vivo kinetics) at 241 u, whereas 1MH was monitored at 340 u and eluted 0.5 min later than 3MH. Standard curves for plasma analysis were linear and nanamole amounts of 3MH in plasma were determined with a precision of 3.5%. 3MH was also quantitated in urine; however, because of substantial amounts of 1MH, (18O2)1MH was used as the internal standard. Nanamole amounts of 3MH were determined in urine with a precision of 2.7%. Application of the 3MH analytical method was used to develop a kinetic compartmental model by using the stable isotope of 3MH, d3-3MH. Cattle, like humans, quantitatively excrete 3MH in the urine. A young bovine was injected with d3-3MH and the enrichment curve in plasma was evaluated in order to obtain a steady-state production rate of 3MH. The decay curve was modeled through the use of NIH-SAAM modeling program. The kinetics of d3-3MH from plasma were adequately described by a three-pool compartmental model. The de novo production rate of 3MH estimated in the calf was 665 mumol per day. This corresponded to an estimated fractional turnover rate of 1.56% per day, which was similar to estimates obtained from urine collections.(ABSTRACT TRUNCATED AT 250 WORDS)

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Synthesis of (S)‐N^τ‐[D₃] methylhistidine

Yuan

Ajami

1984

Labelled Comp Radiopharmac

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S)-Histidine methyl ester was converted on a 20 g scale to its NU, N T -cyclic urea by treatment with carbonyl diimidazole. The N T position was alkylated with [D3]-methyl iodide and the product was hydrolyzed to give isomerically and isotopically pure W -LO3] methylhistidine, without significant loss of optical activity. Key words : deuteri um, carbonyl di imidazole, (S)-5-0~0-7-carbomethoxy-5,6,7,8tetra hydr o imi dazo 1.1 , 5-c I pyr i mi d i ne , ( S ) -N T-met hy 1 hi st i d i ne .

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Picomole assay for Nτ-methylhistidine by gas chromatography—Mass spectrometry

Cited by 20 publications

References 22 publications

Protein and substrate metabolism during starvation and parenteral refeeding

Protein and substrate metabolism during starvation and parenteral refeeding

Gas chromatographic/mass spectrometric analysis of stable isotopes of 3-methylhistidine in biological fluids: Application to plasma kineticsin vivo

Synthesis of (S)‐N^τ‐[D₃] methylhistidine

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Picomole assay for Nτ-methylhistidine by gas chromatography—Mass spectrometry

Cited by 20 publications

References 22 publications

Protein and substrate metabolism during starvation and parenteral refeeding

Protein and substrate metabolism during starvation and parenteral refeeding

Gas chromatographic/mass spectrometric analysis of stable isotopes of 3-methylhistidine in biological fluids: Application to plasma kineticsin vivo

Synthesis of (S)‐Nτ‐[D3] methylhistidine

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Synthesis of (S)‐N^τ‐[D₃] methylhistidine